Display options
Cite
Ampaire LM, Katawera V, Nyehangane D, et al. Epidemiology of Carbapenem Resistance among Multi-drug Resistant Enterobacteriaceae in Uganda. Br Microbiol Res J. 2015;8(2):418-423doi: 10.9734/BMRJ/2015/17055.
Ampaire, L. M., Katawera, V., Nyehangane, D., Boum, Y., & Bazira, J. (2015). Epidemiology of Carbapenem Resistance among Multi-drug Resistant Enterobacteriaceae in Uganda. British microbiology research journal, 8(2), 418-423. https://doi.org/10.9734/BMRJ/2015/17055
Ampaire, Lucas M, et al. "Epidemiology of Carbapenem Resistance among Multi-drug Resistant Enterobacteriaceae in Uganda." British microbiology research journal vol. 8,2 (2015): 418-423. doi: https://doi.org/10.9734/BMRJ/2015/17055
Ampaire LM, Katawera V, Nyehangane D, Boum Y, Bazira J. Epidemiology of Carbapenem Resistance among Multi-drug Resistant Enterobacteriaceae in Uganda. Br Microbiol Res J. 2015;8(2):418-423. doi: 10.9734/BMRJ/2015/17055. Epub 2015 May 02. PMID: 26605152; PMCID: PMC4655185.
Copy Download .nbib Format: NLM
Share it on

Br Microbiol Res J. 2015;8(2):418-423. doi: 10.9734/BMRJ/2015/17055. Epub 2015 May 02.

Epidemiology of Carbapenem Resistance among Multi-drug Resistant Enterobacteriaceae in Uganda.

British microbiology research journal

Lucas M Ampaire, Victoria Katawera, Dan Nyehangane, Yap Boum, Joel Bazira

Affiliations

  1. Department of Medical Laboratory Sciences, Mbarara University of Science and Technology, Mbarara, Uganda.
  2. Department of Laboratory, Epicentre Mbarara Research Centre, Mbarara, Uganda ; Department of Microbiology and Immunology, Mbarara University of Science and Technology, Mbarara, Uganda.
  3. Department of Microbiology and Immunology, Mbarara University of Science and Technology, Mbarara, Uganda.
  4. Department of Medical Laboratory Sciences, Mbarara University of Science and Technology, Mbarara, Uganda ; Department of Laboratory, Epicentre Mbarara Research Centre, Mbarara, Uganda.

PMID: 26605152 PMCID: PMC4655185 DOI: 10.9734/BMRJ/2015/17055

Abstract

BACKGROUND: Multi-drug resistant (MDR) Enterobacteriaceae are on the increase worldwide and their spread has become a global challenge. Escalating the challenge is the possibility that many of these are Carbapenemase-producing Enterobacteriaceae (CPE). This further complicates patient management. The magnitude of MDR-CPE in many developed settings has been reported, however, there is paucity of data from resource limited settings. We evaluated the epidemiology of MDR-CPE of clinical origin in South Western Uganda.

METHODS: From September 2013 to June 2014, all Enterobacteriaceae isolated from diverse specimens obtained from patients attending Mbarara Regional Referral Hospital, South-western Uganda, were screened for MDR in a laboratory-based cross sectional study. Isolates found to be MDR were screened for carbapenem susceptibility/resistance phenotypically by Kirby Bauer disc diffusion method following CLSI guidelines and genetically using the multiplex real-time Polymerase Chain Reaction (RT-PCR).

RESULTS: Of the 658 strains isolated, 183 (27.8%) were MDR and 68 (37.15%) of those MDR exhibited at least one form of carbapenem resistance with 23 (12.57%) and 56 (30.60%) isolates expressing phenotypic and genetic resistance, respectively. Eleven MDR-CPE (6.01%) isolates exhibited both phenotypic and genotypic resistance to carbapenems. Only

CONCLUSION: The high prevalence of MDR-CPE calls for aggressive infection control and prevention strategies, including reinforcement of hand hygiene, using contact precautions and early detection of CPE through use of targeted surveillance and molecular techniques in resource limited settings.

Keywords: CPE; ESBL; Enterobacteriaceae; carbapenemase; multi-drug resistant

References

  1. J Clin Microbiol. 2007 Feb;45(2):544-7 - PubMed
  2. Curr Opin Pharmacol. 2007 Oct;7(5):459-69 - PubMed
  3. Biomed Res Int. 2014;2014:303104 - PubMed
  4. Clin Microbiol Rev. 2007 Jul;20(3):440-58, table of contents - PubMed
  5. Rev Panam Salud Publica. 2011 Dec;30(6):519-28 - PubMed
  6. J Antimicrob Chemother. 2011 Jun;66(6):1255-9 - PubMed
  7. Emerg Infect Dis. 2011 Oct;17(10):1791-8 - PubMed
  8. Diagn Microbiol Infect Dis. 2007 Jan;57(1):85-91 - PubMed
  9. Lancet Infect Dis. 2008 Mar;8(3):159-66 - PubMed
  10. Clin Infect Dis. 2004 Jul 1;39(1):55-60 - PubMed
  11. J Chemother. 2013 Jun;25(3):129-40 - PubMed
  12. Clin Microbiol Infect. 2012 May;18(5):413-31 - PubMed
  13. MMWR Morb Mortal Wkly Rep. 2010 Sep 24;59(37):1212 - PubMed
  14. Antimicrob Agents Chemother. 2011 Nov;55(11):4943-60 - PubMed

Publication Types

Grant support