Display options
Cite
Koessler J, Weber K, Koessler A, et al. Expression and function of purinergic receptors in platelets from apheresis-derived platelet concentrates. Blood Transfus. 2015;14(6):545-551doi: 10.2450/2015.0073-15.
Koessler, J., Weber, K., Koessler, A., Yilmaz, P., Boeck, M., & Kobsar, A. (2016). Expression and function of purinergic receptors in platelets from apheresis-derived platelet concentrates. Blood transfusion = Trasfusione del sangue, 14(6), 545-551. https://doi.org/10.2450/2015.0073-15
Koessler, Juergen, et al. "Expression and function of purinergic receptors in platelets from apheresis-derived platelet concentrates." Blood transfusion = Trasfusione del sangue vol. 14,6 (2016): 545-551. doi: https://doi.org/10.2450/2015.0073-15
Koessler J, Weber K, Koessler A, Yilmaz P, Boeck M, Kobsar A. Expression and function of purinergic receptors in platelets from apheresis-derived platelet concentrates. Blood Transfus. 2016 Nov;14(6):545-551. doi: 10.2450/2015.0073-15. Epub 2015 Nov 17. PMID: 26674810; PMCID: PMC5111382.
Copy Download .nbib Format: NLM
Share it on

Blood Transfus. 2016 Nov;14(6):545-551. doi: 10.2450/2015.0073-15. Epub 2015 Nov 17.

Expression and function of purinergic receptors in platelets from apheresis-derived platelet concentrates.

Blood transfusion = Trasfusione del sangue

Juergen Koessler, Katja Weber, Angela Koessler, Pinar Yilmaz, Markus Boeck, Anna Kobsar

Affiliations

  1. Institute of Transfusion Medicine and Haemotherapy, University of Würzburg, Würzburg, Germany.

PMID: 26674810 PMCID: PMC5111382 DOI: 10.2450/2015.0073-15

Abstract

BACKGROUND: The storage of platelets affects platelet integrity and functionality, a process named platelet storage lesion (PSL). Reduced adenosine diphosphate (ADP)-induced platelet aggregation is a typical manifestation of PSL. However, the role of ADP receptors in this context has not been evaluated yet. The aim of this study was, therefore, to investigate surface expression and function of the purinergic receptors P2Y1, P2Y12 and P2X1 in stored platelet concentrates.

MATERIAL AND METHODS: Platelets were obtained from venous whole blood and from apheresis-derived platelet concentrates stored for 0, 2 and 5 days. Purinergic receptor expression was measured by flow cytometry and western blot analysis. Receptor function was determined by calcium-induced fluorescence (P2Y1 and P2X1) or by flow cytometric measurement of the platelet reactivity index (P2Y12).

RESULTS: The basal surface expression and total content of purinergic receptors remained unchanged throughout storage. After an initial reduction during apheresis, P2X1-mediated calcium flux was maintained, whereas the P2Y1-mediated increase of calcium flux gradually decreased during the course of storage. In contrast, the platelet reactivity index was comparable in freshly obtained and stored platelets.

DISCUSSION: The function of the P2Y12 receptor is maintained during storage of apheresis-derived platelet concentrates. However, the impairment of P2X1 and especially of P2Y1 receptor function indicated by decreased receptor-mediated calcium flux is an important mechanism contributing to reduced ADP responsiveness of stored platelets.

Conflict of interest statement

The Authors declare no conflict of interest.

References

  1. Transfusion. 2012 Jun;52(6):1253-9 - PubMed
  2. Blood. 1988 Aug;72(2):766-9 - PubMed
  3. ScientificWorldJournal. 2002 Feb 13;2:424-33 - PubMed
  4. J Clin Invest. 1971 Feb;50(2):370-7 - PubMed
  5. Blood. 2011 Feb 17;117(7):2102-12 - PubMed
  6. Transfus Sci. 1999 Apr;20(2):129-39 - PubMed
  7. Transfusion. 2014 Jul;54(7):1782-9 - PubMed
  8. Br J Pharmacol. 2002 Jan;135(2):363-72 - PubMed
  9. Curr Opin Pharmacol. 2003 Apr;3(2):175-80 - PubMed
  10. Transfusion. 2004 Jan;44(1):49-58 - PubMed
  11. Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):8070-4 - PubMed
  12. Arterioscler Thromb Vasc Biol. 2015 Jun;35(6):1327-38 - PubMed
  13. Coron Artery Dis. 2012 Sep;23(6):368-74 - PubMed
  14. Transfusion. 1997 Apr;37(4):368-75 - PubMed
  15. Platelets. 1993;4(6):338-40 - PubMed
  16. Blood. 2003 Feb 15;101(4):1400-8 - PubMed
  17. J Am Coll Cardiol. 2013 Dec 17;62(24):2261-73 - PubMed
  18. Clin Chem. 2005 Jun;51(6):957-65 - PubMed
  19. Thromb Haemost. 1999 Sep;82(3):1145-52 - PubMed
  20. Thromb Haemost. 2006 Dec;96(6):767-73 - PubMed
  21. Transfusion. 1997 Jan;37(1):12-7 - PubMed
  22. Transfus Med Rev. 2007 Oct;21(4):287-94 - PubMed
  23. Transfusion. 2000 Oct;40(10):1257-63 - PubMed
  24. Anal Biochem. 2006 Oct 15;357(2):216-24 - PubMed
  25. J Proteomics. 2012 Dec 5;76 Spec No.:297-315 - PubMed
  26. Transfusion. 2014 Apr;54(4):1008-14 - PubMed
  27. Platelets. 2015;26(2):154-63 - PubMed
  28. Transfus Med. 2002 Oct;12(5):317-24 - PubMed
  29. Haemostasis. 1994 Jul-Aug;24(4):230-5 - PubMed
  30. J Pharmacol Exp Ther. 2011 Oct;339(1):54-61 - PubMed
  31. Cardiovasc Hematol Agents Med Chem. 2013 Jun;11(2):101-5 - PubMed
  32. Curr Pharm Des. 2012;18(33):5240-60 - PubMed
  33. Blood. 1982 Mar;59(3):563-8 - PubMed

Publication Types