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Villa F, Malovini A, Carrizzo A, et al. Serum BPIFB4 levels classify health status in long-living individuals. Immun Ageing. 2015;12:27doi: 10.1186/s12979-015-0054-8.
Villa, F., Malovini, A., Carrizzo, A., Spinelli, C. C., Ferrario, A., Maciąg, A., Madonna, M., Bellazzi, R., Milanesi, L., Vecchione, C., & Puca, A. A. (2015). Serum BPIFB4 levels classify health status in long-living individuals. Immunity & ageing : I & A, 1227. https://doi.org/10.1186/s12979-015-0054-8
Villa, Francesco, et al. "Serum BPIFB4 levels classify health status in long-living individuals." Immunity & ageing : I & A vol. 12 (2015): 27. doi: https://doi.org/10.1186/s12979-015-0054-8
Villa F, Malovini A, Carrizzo A, Spinelli CC, Ferrario A, Maciąg A, Madonna M, Bellazzi R, Milanesi L, Vecchione C, Puca AA. Serum BPIFB4 levels classify health status in long-living individuals. Immun Ageing. 2015 Dec 15;12:27. doi: 10.1186/s12979-015-0054-8. eCollection 2015. PMID: 26675039; PMCID: PMC4678610.
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Immun Ageing. 2015 Dec 15;12:27. doi: 10.1186/s12979-015-0054-8. eCollection 2015.

Serum BPIFB4 levels classify health status in long-living individuals.

Immunity & ageing : I & A

Francesco Villa, Alberto Malovini, Albino Carrizzo, Chiara C Spinelli, Anna Ferrario, Anna Maciąg, Michele Madonna, Riccardo Bellazzi, Luciano Milanesi, Carmine Vecchione, Annibale A Puca

Affiliations

  1. Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, 20090 Segrate, MI Italy.
  2. Dipartimento di Ingegneria Industriale e Informatica, Università di Pavia, 27100 Pavia, Italy.
  3. IRCCS Neuromed - Parco Tecnologico, 86077 Pozzilli, IS Italy.
  4. IRCCS MultiMedica, 20138 Milan, Italy.
  5. IRCCS Neuromed - Parco Tecnologico, 86077 Pozzilli, IS Italy ; Dipartimento di Medicina e Chirurgia, Università degli Studi di Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, SA Italy.
  6. IRCCS MultiMedica, 20138 Milan, Italy ; Dipartimento di Medicina e Chirurgia, Università degli Studi di Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, SA Italy.

PMID: 26675039 PMCID: PMC4678610 DOI: 10.1186/s12979-015-0054-8

Abstract

BACKGROUND: People that reach extreme ages (Long-Living Individuals, LLIs) are object of intense investigation for increase/decrease of genetic variant frequencies, genetic methylation levels, protein abundance in serum and tissues. The aim of these studies is the discovery of the mechanisms behind LLIs extreme longevity and the identification of markers of well-being. We have recently associated a BPIFB4 haplotype (LAV) with exceptional longevity under a homozygous genetic model, and identified that CD34(+) of LLIs subjects express higher BPIFB4 transcript as compared to CD34(+) of control population. It would be of interest to correlate serum BPIFB4 protein levels with exceptional longevity and health status of LLIs.

METHODS: Western blots on cellular medium to detect BPIFB4 secretion in transfected HEK293T cells with plasmid carrying BPIFB4 and ELISA on LLIs serum to detect BPIFB4 levels.

RESULTS: Here we show that BPIFB4 is a secreted protein and its levels are increased in serum of LLIs, and high BPIFB4 levels classify their health status.

CONCLUSIONS: Serum BPIFB4 protein levels classify longevity and health status in LLIs. Further studies are required to evaluate the possible role of BPIFB4 in monitoring disease progression.

Keywords: BPIFB4; CD34; Methylation; Vascular ageing

References

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