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Shahani S, Rostamnezhad M, Ghaffari-Rad V, et al. Radioprotective Effect of Achillea millefolium L Against Genotoxicity Induced by Ionizing Radiation in Human Normal Lymphocytes. Dose Response. 2015;13(1):1559325815583761doi: 10.1177/1559325815583761.
Shahani, S., Rostamnezhad, M., Ghaffari-Rad, V., Ghasemi, A., Allahverdi Pourfallah, T., & Hosseinimehr, S. J. (2015). Radioprotective Effect of Achillea millefolium L Against Genotoxicity Induced by Ionizing Radiation in Human Normal Lymphocytes. Dose-response : a publication of International Hormesis Society, 13(1), 1559325815583761. https://doi.org/10.1177/1559325815583761
Shahani, Somayeh, et al. "Radioprotective Effect of Achillea millefolium L Against Genotoxicity Induced by Ionizing Radiation in Human Normal Lymphocytes." Dose-response : a publication of International Hormesis Society vol. 13,1 (2015): 1559325815583761. doi: https://doi.org/10.1177/1559325815583761
Shahani S, Rostamnezhad M, Ghaffari-Rad V, Ghasemi A, Allahverdi Pourfallah T, Hosseinimehr SJ. Radioprotective Effect of Achillea millefolium L Against Genotoxicity Induced by Ionizing Radiation in Human Normal Lymphocytes. Dose Response. 2015 Apr 29;13(1):1559325815583761. doi: 10.1177/1559325815583761. eCollection 2015. PMID: 26675116; PMCID: PMC4674217.
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Dose Response. 2015 Apr 29;13(1):1559325815583761. doi: 10.1177/1559325815583761. eCollection 2015.

Radioprotective Effect of Achillea millefolium L Against Genotoxicity Induced by Ionizing Radiation in Human Normal Lymphocytes.

Dose-response : a publication of International Hormesis Society

Somayeh Shahani, Mostafa Rostamnezhad, Vahid Ghaffari-Rad, Arash Ghasemi, Tayyeb Allahverdi Pourfallah, Seyed Jalal Hosseinimehr

Affiliations

  1. Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
  2. Department of Radiopharmacy, Faculty of Pharmacy, Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran ; Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.
  3. Department of Radiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  4. Department of Biochemistry and Biophysics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  5. Department of Radiopharmacy, Faculty of Pharmacy, Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran.

PMID: 26675116 PMCID: PMC4674217 DOI: 10.1177/1559325815583761

Abstract

The radioprotective effect of Achillea millefolium L (ACM) extract was investigated against genotoxicity induced by ionizing radiation (IR) in human lymphocytes. Peripheral blood samples were collected from human volunteers and incubated with the methanolic extract of ACM at different concentrations (10, 50, 100, and 200 μg/mL) for 2 hours. At each dose point, the whole blood was exposed in vitro to 2.5 Gy of X-ray and then the lymphocytes were cultured with mitogenic stimulation to determine the micronuclei in cytokinesis-blocked binucleated cell. Antioxidant capacity of the extract was determined using free radical-scavenging method. The treatment of lymphocytes with the extract showed a significant decrease in the incidence of micronuclei binucleated cells, as compared with similarly irradiated lymphocytes without any extract treatment. The maximum protection and decrease in frequency of micronuclei were observed at 200 μg/mL of ACM extract which completely protected genotoxicity induced by IR in human lymphocytes. Achillea millefolium extract exhibited concentration-dependent radical-scavenging activity on 1,1-diphenyl-2-picryl hydrazyl free radicals. These data suggest that the methanolic extract of ACM may play an important role in the protection of normal tissues against genetic damage induced by IR.

Keywords: Achillea millefolium; antioxidant; genotoxicity; micronuclei; radioprotective

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