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Khanna A, Khanna M, Gill KS. Comparative Evaluation of Tubex TF (Inhibition Magnetic Binding Immunoassay) for Typhoid Fever in Endemic Area. J Clin Diagn Res. 2015;9(11):DC14-7doi: 10.7860/JCDR/2015/15459.6810.
Khanna, A., Khanna, M., & Gill, K. S. (2015). Comparative Evaluation of Tubex TF (Inhibition Magnetic Binding Immunoassay) for Typhoid Fever in Endemic Area. Journal of clinical and diagnostic research : JCDR, 9(11), DC14-7. https://doi.org/10.7860/JCDR/2015/15459.6810
Khanna, Ashish, et al. "Comparative Evaluation of Tubex TF (Inhibition Magnetic Binding Immunoassay) for Typhoid Fever in Endemic Area." Journal of clinical and diagnostic research : JCDR vol. 9,11 (2015): DC14-7. doi: https://doi.org/10.7860/JCDR/2015/15459.6810
Khanna A, Khanna M, Gill KS. Comparative Evaluation of Tubex TF (Inhibition Magnetic Binding Immunoassay) for Typhoid Fever in Endemic Area. J Clin Diagn Res. 2015 Nov;9(11):DC14-7. doi: 10.7860/JCDR/2015/15459.6810. Epub 2015 Nov 01. PMID: 26676104; PMCID: PMC4668408.
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J Clin Diagn Res. 2015 Nov;9(11):DC14-7. doi: 10.7860/JCDR/2015/15459.6810. Epub 2015 Nov 01.

Comparative Evaluation of Tubex TF (Inhibition Magnetic Binding Immunoassay) for Typhoid Fever in Endemic Area.

Journal of clinical and diagnostic research : JCDR

Ashish Khanna, Menka Khanna, Karamjit Singh Gill

Affiliations

  1. Associate Professor, Department of Microbiology, Sri Guru Ram Das Institute of Medical Sciences and Research , Punjab, India .
  2. Professor, Department of Pathology, Sri Guru Ram Das Institute of Medical Sciences and Research , Punjab, India .
  3. Professor, Department of Pathology, Sri Guru Ram Das Institute of Medical Sciences and Research and Consultant Nijjer labs and Diagnostic Centre , Punjab, India .

PMID: 26676104 PMCID: PMC4668408 DOI: 10.7860/JCDR/2015/15459.6810

Abstract

BACKGROUND: Typhoid fever remains a significant health problem in endemic countries like India. Various serological tests for the diagnosis of typhoid fever are available commercially. We assessed the usefulness of rapid test based on magnetic particle separation to detect Immunoglobulin against Salmonella typhi O9 lipopolysaccharide.

AIM: Aim of this study was to compare the sensitivity and specificity of widal test, typhidot and tubex TF test for the diagnosis of typhoid fever in an endemic country like India.

MATERIALS AND METHODS: Serum samples collected from 50 patients of typhoid fever, 50 patients of non typhoid fever and 100 normal healthy individuals residing in Amritsar were subjected to widal test, typhidot test and tubex TF test as per manufacturer's instructions. Data collected was assessed to find sensitivity and specificity of these tests in an endemic area.

RESULTS: Significant widal test results were found positive in 68% of patients of typhoid fever and only 4% of non typhoid fever patients. Typhidot (IgM or IgG) was positive in 72% of typhoid fever patients and 10% and 6% in non typhoid fever and normal healthy individuals respectively. Tubex TF showed higher sensitivity of 76% and specificity of 96-99% which was higher than typhidot and comparable to widal test.

CONCLUSION: This was the first evaluation of rapid tubex TF test in northern India. In countries which can afford high cost of test, tubex TF should be recommended for the diagnosis in acute stage of the disease in clinical setting. However, there is urgent need for a highly specific and sensitive test for the diagnosis of typhoid fever in clinical settings in endemic areas.

Keywords: Salmonella; Widal test

References

  1. J Clin Microbiol. 1998 Aug;36(8):2271-8 - PubMed
  2. J Clin Pathol. 1983 Apr;36(4):471-5 - PubMed
  3. J Clin Microbiol. 2004 May;42(5):1885-9 - PubMed
  4. PLoS One. 2013 Dec 16;8(12):e81263 - PubMed
  5. Epidemiol Infect. 2007 Aug;135(6):1014-20 - PubMed
  6. J Clin Microbiol. 2007 Jan;45(1):246-7 - PubMed
  7. Lancet. 1975 May 31;1(7918):1211-3 - PubMed
  8. J Infect Dev Ctries. 2011 Mar 21;5(3):169-75 - PubMed
  9. Diagn Microbiol Infect Dis. 2008 Aug;61(4):381-6 - PubMed
  10. Indian J Med Res. 1969 Oct;57(10):1813-9 - PubMed
  11. J Clin Microbiol. 2005 Sep;43(9):4889-90 - PubMed
  12. Clin Vaccine Immunol. 2012 Nov;19(11):1833-7 - PubMed
  13. BMC Res Notes. 2015 Feb 24;8:50 - PubMed
  14. Diagn Microbiol Infect Dis. 2006 Dec;56(4):359-65 - PubMed

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