Display options
Cite
Nishimoto S, Fujita K, Sotsuka Y, et al. Growth Factor Measurement and Histological Analysis in Platelet Rich Fibrin: A Pilot Study. J Maxillofac Oral Surg. 2015;14(4):907-13doi: 10.1007/s12663-015-0768-3.
Nishimoto, S., Fujita, K., Sotsuka, Y., Kinoshita, M., Fujiwara, T., Kawai, K., & Kakibuchi, M. (2015). Growth Factor Measurement and Histological Analysis in Platelet Rich Fibrin: A Pilot Study. Journal of maxillofacial and oral surgery, 14(4), 907-13. https://doi.org/10.1007/s12663-015-0768-3
Nishimoto, Soh, et al. "Growth Factor Measurement and Histological Analysis in Platelet Rich Fibrin: A Pilot Study." Journal of maxillofacial and oral surgery vol. 14,4 (2015): 907-13. doi: https://doi.org/10.1007/s12663-015-0768-3
Nishimoto S, Fujita K, Sotsuka Y, Kinoshita M, Fujiwara T, Kawai K, Kakibuchi M. Growth Factor Measurement and Histological Analysis in Platelet Rich Fibrin: A Pilot Study. J Maxillofac Oral Surg. 2015 Dec;14(4):907-13. doi: 10.1007/s12663-015-0768-3. Epub 2015 Mar 14. PMID: 26604462; PMCID: PMC4648775.
Copy Download .nbib Format: NLM
Share it on

J Maxillofac Oral Surg. 2015 Dec;14(4):907-13. doi: 10.1007/s12663-015-0768-3. Epub 2015 Mar 14.

Growth Factor Measurement and Histological Analysis in Platelet Rich Fibrin: A Pilot Study.

Journal of maxillofacial and oral surgery

Soh Nishimoto, Kazutoshi Fujita, Yohei Sotsuka, Masato Kinoshita, Toshihiro Fujiwara, Kenichiro Kawai, Masao Kakibuchi

Affiliations

  1. Department of Plastic Surgery, Hyogo College of Medicine, Mukogawa-cho 1-1, Nishinomiya, Hyogo 663-8501 Japan.

PMID: 26604462 PMCID: PMC4648775 DOI: 10.1007/s12663-015-0768-3

Abstract

OBJECTIVE: The aim of this study was to compare growth factor amount contained in platelet rich fibrin (PRF) and compare with that in platelet rich plasma (PRP), and in whole blood. And also to investigate distribution of growth factors and cellular components in PRF.

MATERIALS AND METHODS: PRF and PRP were obtained from the same sample of peripheral blood. Extraction of proteins were done with lysis buffer, accompanied by freeze and thaw procedures. Concentration of two representative growth factors in platelets: platelet derived growth factor (PDGF) and transforming growth factor beta (TGF-β), were measured with enzyme-linked immunosorbent assay (ELISA). PRF was cut into three parts: (top, middle and bottom), and growth factor concentration was measured respectively. Paraffin embedded section of PRF was observed with Giemsa stain. Immuno-histochemical analysis with anti-PDGF and anti-TGF-β antibodies was also conducted.

RESULTS: The growth factor levels in PRF was higher than in peripheral blood and comparable to those in PRP. Growth factor levels in bottom part of PRF was much higher than in top and middle part. Microscopically, platelets and mono-nucleated cells were concentrated just above the yellow-red interface. Poly-nucleated cells were concentrated below the interface.

CONCLUSION: The growth factors were surely concentrated in PRF. This result can support basis of good clinical outcomes. For effective application of PRF, the knowledge that growth factors and cells are not equally distributed in PRF should be utilized.

Keywords: ELISA; Growth factor; Immuno-histochemistry; Mono-nucleated cells; Platelet rich fibrin

References

  1. Am J Med. 1958 Aug;25(2):160-8 - PubMed
  2. J Maxillofac Oral Surg. 2012 Dec;11(4):430-4 - PubMed
  3. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998 Jun;85(6):638-46 - PubMed
  4. J Craniofac Surg. 2011 Sep;22(5):1951-5 - PubMed
  5. J Oral Maxillofac Surg. 2004 May;62(5):555-8 - PubMed
  6. J Periodontol. 2011 Dec;82(12 ):1705-12 - PubMed
  7. Metabolism. 1979 Apr;28(4 Suppl 1):410-4 - PubMed
  8. J Biol Chem. 1983 Jun 10;258(11):7155-60 - PubMed
  9. Plast Reconstr Surg. 2012 Dec;130(6):819e-829e - PubMed
  10. Wound Repair Regen. 2007 Jan-Feb;15(1):156-62 - PubMed
  11. J Clin Invest. 1968 Dec;47(12):2608-15 - PubMed
  12. Plast Reconstr Surg. 2004 Nov;114(6):1502-8 - PubMed
  13. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Mar;101(3):e45-50 - PubMed
  14. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Mar;101(3):e37-44 - PubMed
  15. Ann Maxillofac Surg. 2011 Jan;1(1):53-7 - PubMed
  16. J Physiol. 1957 Jun 18;137(1):95-109 - PubMed
  17. J Maxillofac Oral Surg. 2013 Mar;12(1):11-6 - PubMed
  18. Clin Oral Implants Res. 2004 Apr;15(2):187-93 - PubMed
  19. Transfus Apher Sci. 2004 Apr;30(2):145-51 - PubMed

Publication Types