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Siqueira PC, Magalhães AP, Pires WC, et al. Cytotoxicity of glass ionomer cements containing silver nanoparticles. J Clin Exp Dent. 2015;7(5):e622-7doi: 10.4317/jced.52566.
Siqueira, P. C., Magalhães, A. P., Pires, W. C., Pereira, F. C., Silveira-Lacerda, E. P., Carrião, M. S., Bakuzis, A. F., Souza-Costa, C. A., Lopes, L. G., & Estrela, C. (2015). Cytotoxicity of glass ionomer cements containing silver nanoparticles. Journal of clinical and experimental dentistry, 7(5), e622-7. https://doi.org/10.4317/jced.52566
Siqueira, Patrícia-Correia, et al. "Cytotoxicity of glass ionomer cements containing silver nanoparticles." Journal of clinical and experimental dentistry vol. 7,5 (2015): e622-7. doi: https://doi.org/10.4317/jced.52566
Siqueira PC, Magalhães AP, Pires WC, Pereira FC, Silveira-Lacerda EP, Carrião MS, Bakuzis AF, Souza-Costa CA, Lopes LG, Estrela C. Cytotoxicity of glass ionomer cements containing silver nanoparticles. J Clin Exp Dent. 2015 Dec 01;7(5):e622-7. doi: 10.4317/jced.52566. eCollection 2015 Dec. PMID: 26644839; PMCID: PMC4663065.
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J Clin Exp Dent. 2015 Dec 01;7(5):e622-7. doi: 10.4317/jced.52566. eCollection 2015 Dec.

Cytotoxicity of glass ionomer cements containing silver nanoparticles.

Journal of clinical and experimental dentistry

Patrícia-Correia Siqueira, Ana-Paula-Rodrigues Magalhães, Wanessa-Carvalho Pires, Flávia-Castro Pereira, Elisângela-Paula Silveira-Lacerda, Marcus-Santos Carrião, Andris-Figueiroa Bakuzis, Carlos-Alberto Souza-Costa, Lawrence-Gonzaga Lopes, Carlos Estrela

Affiliations

  1. DDS, MSc. Department of Stomatological Sciences, School of Dentistry, Federal University of Goiás, Goiânia, Goiás, Brazil.
  2. DDS, MSc. Department of Prevention and Oral Rehabilitation, School of Dentistry, Federal University of Goiás,Goiânia, Goiás, Brazil.
  3. MSc. Laboratory of Molecular Genetics and Cytogenetics, Federal University of Goiás,Goiânia, Goiás, Brazil.
  4. MSc, PhD. Laboratory of Molecular Genetics and Cytogenetics, Federal University of Goiás, Goiânia, Goiás, Brazil.
  5. MSc. Physics Institute, Federal University of Goiás, Goiânia, Goiás, Brazil.
  6. MSc, PhD. Physics Institute, Federal University of Goiás, Goiânia, Goiás, Brazil.
  7. DDS, MSc, PhD. Department of Physiology and Pathology, School of Dentistry (UNESP), Araraquara, São Paulo, Brazil.
  8. DDS, MSc, PhD. Department of Prevention and Oral Rehabilitation, School of Dentistry, Federal University of Goiás, Goiânia, Goiás, Brazil.
  9. DDS, MSc, PhD. Department of Stomatological Sciences, School of Dentistry, Federal University of Goiás, Goiânia, Goiás, Brazil.

PMID: 26644839 PMCID: PMC4663065 DOI: 10.4317/jced.52566

Abstract

BACKGROUND: Some studies have investigated the possibility of incorporating silver nanoparticles (NAg) into dental materials to improve their antibacterial properties. However, the potential toxic effect of this material on pulp cells should be investigated in order to avoid additional damage to the pulp tissue. This study evaluated the cytotoxicity of conventional and resin-modified glass ionomer cements (GIC) with and without addition of NAg.

MATERIAL AND METHODS: NAg were added to the materials at two different concentrations by weight: 0.1% and 0.2%. Specimens with standardized dimensions were prepared, immersed in 400 µL of culture medium and incubated at 37°C and 5% CO2 for 48 h to prepare GIC liquid extracts, which were then incubated in contact with cells for 48 h. Culture medium and 0.78% NAg solution were used as negative and positive controls, respectively. Cell viability was determined by MTT and Trypan Blue assays. ANOVA and the Tukey test (α=0.05) were used for statistical analyses.

RESULTS: Both tests revealed a significant decrease in cell viability in all groups of resin modified cements (p<0.001). There were no statistically significant differences between groups with and without NAg (p>0.05). The differences in cell viability between any group of conventional GIC and the negative control were not statistically significant (p>0.05).

CONCLUSIONS: NAg did not affect the cytotoxicity of the GIC under evaluation.

KEY WORDS: Glass ionomer cements, totoxicity, cell culture techniques, nanotechnology, metal nanoparticles.

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