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Sumsakul W, Chaijaroenkul W, Na-Bangchang K. In vitro inhibitory effects of plumbagin, the promising antimalarial candidate, on human cytochrome P450 enzymes. Asian Pac J Trop Med. 2015;8(11):914-918doi: 10.1016/j.apjtm.2015.10.016.
Sumsakul, W., Chaijaroenkul, W., & Na-Bangchang, K. (2015). In vitro inhibitory effects of plumbagin, the promising antimalarial candidate, on human cytochrome P450 enzymes. Asian Pacific journal of tropical medicine, 8(11), 914-918. https://doi.org/10.1016/j.apjtm.2015.10.016
Sumsakul, Wiriyaporn, et al. "In vitro inhibitory effects of plumbagin, the promising antimalarial candidate, on human cytochrome P450 enzymes." Asian Pacific journal of tropical medicine vol. 8,11 (2015): 914-918. doi: https://doi.org/10.1016/j.apjtm.2015.10.016
Sumsakul W, Chaijaroenkul W, Na-Bangchang K. In vitro inhibitory effects of plumbagin, the promising antimalarial candidate, on human cytochrome P450 enzymes. Asian Pac J Trop Med. 2015 Nov;8(11):914-918. doi: 10.1016/j.apjtm.2015.10.016. Epub 2015 Oct 09. PMID: 26614990.
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Asian Pac J Trop Med. 2015 Nov;8(11):914-918. doi: 10.1016/j.apjtm.2015.10.016. Epub 2015 Oct 09.

In vitro inhibitory effects of plumbagin, the promising antimalarial candidate, on human cytochrome P450 enzymes.

Asian Pacific journal of tropical medicine

Wiriyaporn Sumsakul, Wanna Chaijaroenkul, Kesara Na-Bangchang

Affiliations

  1. Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathumthani 12121, Thailand.
  2. Center of Excellence in Pharmacology and Molecular Biology, Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine, Thammasat University, Pathumthani 12121, Thailand.
  3. Center of Excellence in Pharmacology and Molecular Biology, Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine, Thammasat University, Pathumthani 12121, Thailand. Electronic address: [email protected].

PMID: 26614990 DOI: 10.1016/j.apjtm.2015.10.016

Abstract

OBJECTIVE: To investigate the propensity of plumbagin to inhibit the three isoforms of human cytochrome P450 (CYP), i.e., CYP1A2, CYP2C19, and CYP3A4 using human liver microsomes in vitro.

METHODS: Inhibitory effects of plumbagin on the three human CYP isoforms were investigated using pooled human liver microsomes. Phenacetin O-deethylation, omeprazole hydroxylation and nifedipine oxidation were used as selective substrates for CYP1A2, CYP2C19 and CYP3A4 activities, respectively. Concentrations of paracetamol, 5-hydroxyomeprazole, and oxidized nifedipine were determined in microsomal incubation mixture using high-performance liquid chromatography.

RESULTS: Plumbagin showed significant inhibitory effects on all CYP isoforms, but with the most potent activity on CYP2C19-mediated omeprazole hydroxylation. The IC50 (concentration that inhibits enzyme activity by 50%) values of plumbagin and nootkatone (selective inhibitor) for CYP2C19 were (0.78 ± 0.01) and (27.31 ± 0.66) μM, respectively. The inhibitory activities on CYP1A2-mediated phenacetin O-deethylation and CYP3A4-mediated nifedipine oxidation were moderate. The IC50 values of plumbagin and α-naphthoflavone (selective inhibitor) for CYP1A2 were (1.39 ± 0.01) and (0.02 ± 0.36) μM, respectively. The corresponding IC50 values of plumbagin and ketoconazole (selective inhibitor) for CYP3A4 were (2.37 ± 0.10) and (0.18 ± 0.06) μM, respectively.

CONCLUSIONS: Clinical relevance of the interference of human drug metabolizing enzymes should be aware of for further development scheme of plumbagin as antimalarial drug when used in combination with other antimalarial drugs which are metabolized by these CYP isoforms.

Copyright © 2015 Hainan Medical College. Production and hosting by Elsevier B.V. All rights reserved.

Keywords: Cytochrome P450; Enzyme inhibition; Human liver microsomes; Metabolism; Plumbagin

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