Display options
Cite
Gargano AF, Duffin M, Navarro P, et al. Reducing Dilution and Analysis Time in Online Comprehensive Two-Dimensional Liquid Chromatography by Active Modulation. Anal Chem. 2016;88(3):1785-93doi: 10.1021/acs.analchem.5b04051.
Gargano, A. F., Duffin, M., Navarro, P., & Schoenmakers, P. J. (2016). Reducing Dilution and Analysis Time in Online Comprehensive Two-Dimensional Liquid Chromatography by Active Modulation. Analytical chemistry, 88(3), 1785-93. https://doi.org/10.1021/acs.analchem.5b04051
Gargano, Andrea F G, et al. "Reducing Dilution and Analysis Time in Online Comprehensive Two-Dimensional Liquid Chromatography by Active Modulation." Analytical chemistry vol. 88,3 (2016): 1785-93. doi: https://doi.org/10.1021/acs.analchem.5b04051
Gargano AF, Duffin M, Navarro P, Schoenmakers PJ. Reducing Dilution and Analysis Time in Online Comprehensive Two-Dimensional Liquid Chromatography by Active Modulation. Anal Chem. 2016 Feb 02;88(3):1785-93. doi: 10.1021/acs.analchem.5b04051. Epub 2016 Jan 08. PMID: 26709410; PMCID: PMC5373567.
Copy Download .nbib Format: NLM
Share it on

Anal Chem. 2016 Feb 02;88(3):1785-93. doi: 10.1021/acs.analchem.5b04051. Epub 2016 Jan 08.

Reducing Dilution and Analysis Time in Online Comprehensive Two-Dimensional Liquid Chromatography by Active Modulation.

Analytical chemistry

Andrea F G Gargano, Mike Duffin, Pablo Navarro, Peter J Schoenmakers

Affiliations

  1. TI-COAST, Van 't Hoff Institute for Molecular Sciences, Science Park 904, 1098 XH Amsterdam, Netherlands.
  2. Van 't Hoff Institute for Molecular Sciences, Science Park 904 1098 XH Amsterdam, Netherlands.
  3. Syngenta, Jealott's Hill International Research Centre, Bracknell, Berkshire RG42 6EY, United Kingdom.

PMID: 26709410 PMCID: PMC5373567 DOI: 10.1021/acs.analchem.5b04051

Abstract

Online comprehensive two-dimensional liquid chromatography (LC × LC) offers ways to achieve high-performance separations in terms of peak capacity (exceeding 1000) and additional selectivity to realize applications that cannot be addressed with one-dimensional chromatography (1D-LC). However, the greater resolving power of LC × LC comes at the price of higher dilutions (thus, reduced sensitivity) and, often, long analysis times (>100 min). The need to preserve the separation attained in the first dimension ((1)D) causes greater dilution for LC × LC, in comparison with 1D-LC, and long analysis times to sample the (1)D with an adequate number of second dimension separations. A way to significantly reduce these downsides is to introduce a concentration step between the two chromatographic dimensions. In this work we present a possible active-modulation approach to concentrate the fractions of (1)D effluent. A typical LC × LC system is used with the addition of a dilution flow to decrease the strength of the (1)D effluent and a modulation unit that uses trap columns. The potential of this approach is demonstrated for the separation of tristyrylphenol ethoxylate phosphate surfactants, using a combination of hydrophilic interaction and reversed-phase liquid chromatography. The modified LC × LC system enabled us to halve the analysis time necessary to obtain a similar degree of separation efficiency with respect to UHPLC based LC × LC and of 5 times with respect to HPLC instrumentation (40 compared with 80 and 200 min, respectively), while at the same time reducing dilution (DF of 142, 299, and 1529, respectively) and solvent consumption per analysis (78, 120, and 800 mL, respectively).

References

  1. J Chromatogr A. 2004 Nov 5;1055(1-2):123-33 - PubMed
  2. J Chromatogr A. 2006 Jul 7;1120(1-2):132-41 - PubMed
  3. Anal Chem. 2012 Aug 7;84(15):6747-52 - PubMed
  4. J Sep Sci. 2009 Aug;32(15-16):2530-43 - PubMed
  5. J Chromatogr A. 2006 Jun 30;1119(1-2):3-10 - PubMed
  6. J Sep Sci. 2006 Nov;29(16):2500-13 - PubMed
  7. Anal Chem. 2008 Apr 15;80(8):2756-67 - PubMed
  8. Anal Chem. 2015;87(10):5387-94 - PubMed
  9. Anal Chem. 2010 Oct 15;82(20):8525-36 - PubMed
  10. Anal Chem. 2011 Dec 15;83(24):9531-9 - PubMed
  11. Anal Chem. 2012 Sep 18;84(18):7802-9 - PubMed
  12. J Chromatogr A. 2007 Oct 19;1168(1-2):3-43; discussion 2 - PubMed
  13. J Chromatogr A. 2011 Dec 9;1218(49):8777-90 - PubMed
  14. J Chromatogr A. 2007 May 11;1149(1):73-87 - PubMed
  15. J Chromatogr A. 2014 Sep 19;1360:164-71 - PubMed
  16. J Chromatogr A. 1995 May 26;703(1-2):3-15 - PubMed
  17. J Chromatogr A. 2006 Mar 24;1109(2):253-66 - PubMed
  18. J Chromatogr A. 2011 Apr 15;1218(15):1995-2006 - PubMed
  19. J Chromatogr A. 2015 Feb 13;1381:74-86 - PubMed
  20. Anal Chem. 2005 Oct 1;77(19):6426-34 - PubMed
  21. J Chromatogr A. 2016 Jan 4;1427:90-5 - PubMed
  22. J Sep Sci. 2006 Mar;29(4):492-8 - PubMed
  23. J Sep Sci. 2008 Jun;31(10):1677-85 - PubMed
  24. Anal Chem. 1997 Jul 1;69(13):2283-91 - PubMed
  25. J Sep Sci. 2010 Jun;33(10):1421-37 - PubMed
  26. Anal Chem. 2011 Sep 15;83(18):7053-60 - PubMed
  27. Anal Chem. 2014 Jul 1;86(13):6172-9 - PubMed
  28. J Chromatogr A. 2010 Sep 3;1217(36):5700-9 - PubMed
  29. J Chromatogr A. 2004 Oct 29;1054(1-2):3-16 - PubMed
  30. Anal Chem. 2009 Jan 15;81(2):845-50 - PubMed
  31. Anal Chem. 2008 Jan 15;80(2):461-73 - PubMed
  32. J Sep Sci. 2012 Jul;35(14):1808-20 - PubMed
  33. J Chromatogr A. 2006 Jul 28;1122(1-2):123-37 - PubMed
  34. Biomed Chromatogr. 2006 Jun-Jul;20(6-7):508-29 - PubMed
  35. Anal Chem. 2007 May 15;79(10):3764-70 - PubMed
  36. Anal Chem. 1999 May 1;71(9):1809-14 - PubMed
  37. Anal Chim Acta. 2009 May 8;641(1-2):14-31 - PubMed
  38. Anal Chem. 2010 Aug 15;82(16):7015-20 - PubMed

Publication Types