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New Microbes New Infect. 2015 Oct 02;8:89-98. doi: 10.1016/j.nmni.2015.09.013. eCollection 2015 Nov.

Noncontiguous finished genome sequence and description of Weeksella massiliensis sp. nov.

New microbes and new infections

S A Sankar, C I Lo, B Fall, B Sambe-Ba, O Mediannikov, I Diallo, N Labas, N Faye, B Wade, D Raoult, P-E Fournier, F Fenollar

Affiliations

  1. )Aix-Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, Inserm U1095, Faculté de médecine, Marseille, France.
  2. )Aix-Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, Inserm U1095, Faculté de médecine, Marseille, France ; )Campus International UCAD-IRD, Dakar, Senegal.
  3. )Hôpital Principal, Dakar, Senegal.
  4. )Université Cheikh Anta Diop de Dakar, Laboratoire de Parasitologie générale, Dakar, Senegal.
  5. )Aix-Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, Inserm U1095, Faculté de médecine, Marseille, France ; )Campus International UCAD-IRD, Dakar, Senegal ; )Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.

PMID: 26649182 PMCID: PMC4644262 DOI: 10.1016/j.nmni.2015.09.013

Abstract

Strain FF8(T) (= CSUR P860 = DSM 28259) was isolated in Dakar, Senegal, from the urine of a 65-year-old man with acute cystitis. This strain shows a similarity of sequence of 16S rRNA of 98.38% with Weeksella virosa, and its GenBank accession numbers are HG931340 and CCMH00000000. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis had a poor score, ranging from 1.32 to 1.56, that did not allow identification of the bacterium. Using a polyphasic study made of phenotypic and genomic analyses, strain FF8(T) was a Gram-negative, aerobic rod and a member of the family Flavobacteriaceae. The sequenced genome is 2 562 781 bp with one chromosome but no plasmid. It exhibits a G + C content of 35.9% and contains 2390 protein-coding and 56 RNA genes, including a complete rRNA operon. On the basis of these data, we propose the creation of Weeksella massiliensis sp. nov.

Keywords: Culturomics; Weeksella massiliensis; cystisis; genome; human; taxonogenomics; urine

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