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Nelson LS, Steussy B, Morris CS, et al. Effect of specimen type on free immunoglobulin light chains analysis on the Roche Diagnostics cobas 8000 analyzer. Springerplus. 2015;4:760doi: 10.1186/s40064-015-1546-x.
Nelson, L. S., Steussy, B., Morris, C. S., & Krasowski, M. D. (2015). Effect of specimen type on free immunoglobulin light chains analysis on the Roche Diagnostics cobas 8000 analyzer. SpringerPlus, 4760. https://doi.org/10.1186/s40064-015-1546-x
Nelson, Louis S, et al. "Effect of specimen type on free immunoglobulin light chains analysis on the Roche Diagnostics cobas 8000 analyzer." SpringerPlus vol. 4 (2015): 760. doi: https://doi.org/10.1186/s40064-015-1546-x
Nelson LS, Steussy B, Morris CS, Krasowski MD. Effect of specimen type on free immunoglobulin light chains analysis on the Roche Diagnostics cobas 8000 analyzer. Springerplus. 2015 Dec 08;4:760. doi: 10.1186/s40064-015-1546-x. eCollection 2015. PMID: 26682113; PMCID: PMC4671985.
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Springerplus. 2015 Dec 08;4:760. doi: 10.1186/s40064-015-1546-x. eCollection 2015.

Effect of specimen type on free immunoglobulin light chains analysis on the Roche Diagnostics cobas 8000 analyzer.

SpringerPlus

Louis S Nelson, Bryan Steussy, Cory S Morris, Matthew D Krasowski

Affiliations

  1. Department of Pathology, University of Iowa Hospitals and Clinics, Iowa City, IA 52242 USA.

PMID: 26682113 PMCID: PMC4671985 DOI: 10.1186/s40064-015-1546-x

Abstract

The measurement of free immunoglobulin light chains is typically performed on serum; however, the use of alternative specimen types has potential benefits. Using the Freelite™ kappa and lambda free light chains assay on a Roche Diagnostics cobas 8000 c502 analyzer, we compared three specimen types (serum, EDTA-plasma and lithium heparin plasma separator gel-plasma) on 100 patients. Using Deming regression and eliminating outliers (limiting data to light chain concentrations below 400 mg/L), the three specimen types showed comparable results for kappa light chain concentration, lambda light chain concentration, and kappa/lambda ratio with slopes close to 1.0 and y-intercepts close to zero. EDTA-plasma showed slightly more positive bias relative to serum than lithium heparin. Analysis using EDTA-plasma and lithium heparin plasma showed comparable linearity, precision, and temperature stability. A single sample showing hook effect (not in the comparison set) gave comparable results using either plasma specimen type. For the Freelite™ kappa and lambda free light chains assay, both EDTA-plasma or lithium heparin-plasma can serve as acceptable substitutes for serum, at least for the Roche cobas 8000 analyzer.

Keywords: Immunoglobulin light chains; Laboratory automation; Nephelometry; Plasma; Serum

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