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Shoughy SS, Alkatan HM, Al-Abdullah AA, et al. Polymerase chain reaction in unilateral cases of presumed viral anterior uveitis. Clin Ophthalmol. 2015;9:2325-8doi: 10.2147/OPTH.S93655.
Shoughy, S. S., Alkatan, H. M., Al-Abdullah, A. A., El-Khani, A., de Groot-Mijnes, J. D., & Tabbara, K. F. (2015). Polymerase chain reaction in unilateral cases of presumed viral anterior uveitis. Clinical ophthalmology (Auckland, N.Z.), 92325-8. https://doi.org/10.2147/OPTH.S93655
Shoughy, Samir S, et al. "Polymerase chain reaction in unilateral cases of presumed viral anterior uveitis." Clinical ophthalmology (Auckland, N.Z.) vol. 9 (2015): 2325-8. doi: https://doi.org/10.2147/OPTH.S93655
Shoughy SS, Alkatan HM, Al-Abdullah AA, El-Khani A, de Groot-Mijnes JD, Tabbara KF. Polymerase chain reaction in unilateral cases of presumed viral anterior uveitis. Clin Ophthalmol. 2015 Dec 14;9:2325-8. doi: 10.2147/OPTH.S93655. eCollection 2015. PMID: 26715836; PMCID: PMC4686330.
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Clin Ophthalmol. 2015 Dec 14;9:2325-8. doi: 10.2147/OPTH.S93655. eCollection 2015.

Polymerase chain reaction in unilateral cases of presumed viral anterior uveitis.

Clinical ophthalmology (Auckland, N.Z.)

Samir S Shoughy, Hind M Alkatan, Abdulelah A Al-Abdullah, Albarah El-Khani, Jolanda Df de Groot-Mijnes, Khalid F Tabbara

Affiliations

  1. Department of Ophthalmology, The Eye Center and The Eye Foundation for Research in Ophthalmology, Riyadh, Saudi Arabia.
  2. Department of Pathology & Laboratory Medicine and Uveitis Division, King Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia ; Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
  3. Department of Pathology & Laboratory Medicine and Uveitis Division, King Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia.
  4. Department of Virology and Ophthalmology, University Medical Center Utrecht, Utrecht, the Netherlands.
  5. Department of Ophthalmology, The Eye Center and The Eye Foundation for Research in Ophthalmology, Riyadh, Saudi Arabia ; Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia ; The Wilmer Ophthalmological Institute of The Johns Hopkins University School of Medicine, Baltimore, MD, USA.

PMID: 26715836 PMCID: PMC4686330 DOI: 10.2147/OPTH.S93655

Abstract

BACKGROUND AND OBJECTIVES: Anterior uveitis is the most common form of intraocular inflammation. The main aim of this study was to determine the viral etiology in patients with unilateral cases of anterior uveitis.

PATIENTS AND METHODS: A total of 12 consecutive patients with the diagnosis of idiopathic unilateral anterior uveitis were included prospectively. Aqueous specimens were obtained from each patient by anterior chamber paracentesis and subjected to the detection of viral DNA/RNA genome by polymerase chain reaction assay for herpes simplex virus, varicella zoster virus, cytomegalovirus, Epstein-Barr virus, and rubella virus.

RESULTS: There were six male and six female patients. The mean age was 43 years, with an age range of 11-82 years. All 12 cases presented with unilateral anterior uveitis. In four (33%) patients, polymerase chain reaction was positive for viral genome. Two patients were positive for herpes simplex virus type 1, one patient was positive for cytomegalovirus and one for Epstein-Barr virus.

CONCLUSION: Recent molecular diagnostic assays would help in the identification of the causative agent in patients with unilateral anterior uveitis.

Keywords: PCR; anterior chamber paracentesis; cytomegalovirus; diffuse keratic precipitates; herpes simplex virus; viral anterior uveitis

References

  1. Am J Ophthalmol. 2008 Aug;146(2):292-7 - PubMed
  2. Graefes Arch Clin Exp Ophthalmol. 2010 Oct;248(10):1487-91 - PubMed
  3. Ocul Immunol Inflamm. 2013 Aug;21(4):270-5 - PubMed
  4. Int Ophthalmol. 2010 Oct;30(5):559-65 - PubMed
  5. Br J Ophthalmol. 2006 Jul;90(7):812-3 - PubMed
  6. Arq Bras Oftalmol. 2007 Jan-Feb;70(1):109-14 - PubMed
  7. Eye (Lond). 2007 Jan;21(1):33-9 - PubMed
  8. Br J Ophthalmol. 2008 Jul;92(7):928-32 - PubMed
  9. Ophthalmology. 2008 Feb;115(2):287-91 - PubMed
  10. Am J Ophthalmol. 2006 Feb;141(2):313-8 - PubMed
  11. Am J Ophthalmol. 2010 Nov;150(5):628-36 - PubMed
  12. Am J Ophthalmol. 2007 Nov;144(5):781-5 - PubMed
  13. Curr Eye Res. 2005 Nov;30(11):943-8 - PubMed
  14. Eye (Lond). 2012 Apr;26(4):529-34 - PubMed
  15. Ophthalmic Epidemiol. 2002 Oct;9(4):239-49 - PubMed
  16. Open Ophthalmol J. 2008 Oct 22;2:141-5 - PubMed
  17. Am J Ophthalmol. 2004 Jul;138(1):46-54 - PubMed
  18. Indian J Ophthalmol. 2010 Nov-Dec;58(6):545-7 - PubMed
  19. Br J Ophthalmol. 1995 Jun;79(6):610-6 - PubMed

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