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Gölz G, Karadas G, Fischer A, et al. Toll-Like Receptor-4 is Essential for Arcobacter Butzleri-Induced Colonic and Systemic Immune Responses in Gnotobiotic IL-10(-/-) Mice. Eur J Microbiol Immunol (Bp). 2015;5(4):321-32doi: 10.1556/1886.2015.00043.
Gölz, G., Karadas, G., Fischer, A., Göbel, U. B., Alter, T., Bereswill, S., & Heimesaat, M. M. (2015). Toll-Like Receptor-4 is Essential for Arcobacter Butzleri-Induced Colonic and Systemic Immune Responses in Gnotobiotic IL-10(-/-) Mice. European journal of microbiology & immunology, 5(4), 321-32. https://doi.org/10.1556/1886.2015.00043
Gölz, Greta, et al. "Toll-Like Receptor-4 is Essential for Arcobacter Butzleri-Induced Colonic and Systemic Immune Responses in Gnotobiotic IL-10(-/-) Mice." European journal of microbiology & immunology vol. 5,4 (2015): 321-32. doi: https://doi.org/10.1556/1886.2015.00043
Gölz G, Karadas G, Fischer A, Göbel UB, Alter T, Bereswill S, Heimesaat MM. Toll-Like Receptor-4 is Essential for Arcobacter Butzleri-Induced Colonic and Systemic Immune Responses in Gnotobiotic IL-10(-/-) Mice. Eur J Microbiol Immunol (Bp). 2015 Nov 18;5(4):321-32. doi: 10.1556/1886.2015.00043. eCollection 2015 Dec. PMID: 26716021; PMCID: PMC4681360.
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Eur J Microbiol Immunol (Bp). 2015 Nov 18;5(4):321-32. doi: 10.1556/1886.2015.00043. eCollection 2015 Dec.

Toll-Like Receptor-4 is Essential for Arcobacter Butzleri-Induced Colonic and Systemic Immune Responses in Gnotobiotic IL-10(-/-) Mice.

European journal of microbiology & immunology

Greta Gölz, Gül Karadas, André Fischer, Ulf B Göbel, Thomas Alter, Stefan Bereswill, Markus M Heimesaat

Affiliations

  1. Institute of Food Hygiene, Freie Universität Berlin , Berlin, Germany.
  2. Department of Microbiology and Hygiene, Charité - University Medicine Berlin , Berlin, Germany.

PMID: 26716021 PMCID: PMC4681360 DOI: 10.1556/1886.2015.00043

Abstract

Arcobacter butzleri causes sporadic cases of gastroenteritis, but the underlying immunopathological mechanisms of infection are unknown. We have recently demonstrated that A. butzleri-infected gnotobiotic IL-10(-/-) mice were clinically unaffected but exhibited intestinal and systemic inflammatory immune responses. For the first time, we here investigated the role of Toll-like receptor (TLR)-4, the main receptor for lipopolysaccharide and lipooligosaccharide of Gram-negative bacteria, in murine arcobacteriosis. Gnotobiotic TLR-4/IL-10-double deficient (TLR-4(-/-) IL-10(-/-)) and IL-10(-/-) control mice generated by broad-spectrum antibiotics were perorally infected with A. butzleri. Until day 16 postinfection, mice of either genotype were stably colonized with the pathogen, but fecal bacterial loads were approximately 0.5-2.0 log lower in TLR-4(-/-) IL-10(-/-) as compared to IL-10(-/-) mice. A. butzleri-infected TLR-4(-/-) IL-10(-/-) mice displayed less pronounced colonic apoptosis accompanied by lower numbers of macrophages and monocytes, T lymphocytes, regulatory T-cells, and B lymphocytes within the colonic mucosa and lamina propria as compared to IL-10(-/-) mice. Furthermore, colonic concentrations of nitric oxide, TNF, IL-6, MCP-1, and, remarkably, IFN-γ and IL-12p70 serum levels were lower in A. butzleri-infected TLR-4(-/-) IL-10(-/-) versus IL-10(-/-) mice. In conclusion, TLR-4 is involved in mediating murine A. butzleri infection. Further studies are needed to investigate the molecular mechanisms underlying Arcobacter-host interactions in more detail.

Keywords: Arcobacter butzleri; Toll-like receptor-4; apoptosis; colon; gnotobiotic IL-10–/–; innate and adaptive immunity; lipooligosaccharide; lipopolysaccharide; mice; pro-inflammatory immune responses; systemic immune responses

References

  1. PLoS One. 2011;6(6):e20953 - PubMed
  2. Foodborne Pathog Dis. 2013 Mar;10(3):250-5 - PubMed
  3. Annu Rev Immunol. 2002;20:197-216 - PubMed
  4. J Food Prot. 2002 Dec;65(12):1849-53 - PubMed
  5. Front Cell Infect Microbiol. 2014 Jun 10;4:77 - PubMed
  6. Emerg Infect Dis. 2004 Oct;10(10):1863-7 - PubMed
  7. Eur J Microbiol Immunol (Bp). 2013 Sep;3(3):174-82 - PubMed
  8. Emerg Infect Dis. 2006 Feb;12(2):307-9 - PubMed
  9. PLoS One. 2012;7(7):e40761 - PubMed
  10. J Food Prot. 2003 Aug;66(8):1374-8 - PubMed
  11. Carbohydr Res. 2010 Apr 19;345(6):850-3 - PubMed
  12. New Microbiol. 1997 Jan;20(1):63-8 - PubMed
  13. J Appl Microbiol. 2016 Jan;120(1):218-25 - PubMed
  14. Mar Pollut Bull. 2008 Mar;56(3):600-6 - PubMed
  15. J Immunol. 2006 Dec 15;177(12):8785-95 - PubMed
  16. Gastroenterology. 1999 May;116(5):1107-14 - PubMed
  17. Appl Environ Microbiol. 2013 Aug;79(16):4951-7 - PubMed
  18. Gut. 2010 Aug;59(8):1079-87 - PubMed
  19. Crit Rev Microbiol. 2016 May;42(3):364-83 - PubMed
  20. Poult Sci. 1999 Apr;78(4):536-45 - PubMed
  21. J Clin Microbiol. 1992 Sep;30(9):2335-7 - PubMed
  22. Microb Pathog. 2014 Nov;76:19-25 - PubMed
  23. Infect Immun. 1996 Jun;64(6):2295-9 - PubMed
  24. West Afr J Med. 2012 Jul-Sep;31(3):186-91 - PubMed
  25. Microbiology. 2010 Aug;156(Pt 8):2271-82 - PubMed
  26. Curr Issues Mol Biol. 2006 Jan;8(1):1-10 - PubMed
  27. Eur J Microbiol Immunol (Bp). 2012 Mar;2(1):2-11 - PubMed
  28. J Infect Dis. 2009 Sep 1;200(5):756-64 - PubMed
  29. Clin Microbiol Rev. 2011 Jan;24(1):174-92 - PubMed
  30. PLoS One. 2014 Feb 25;9(2):e90148 - PubMed
  31. Emerg Infect Dis. 2014 Oct;20(10):1731-4 - PubMed
  32. PLoS One. 2015 Sep 25;10(9):e0139402 - PubMed
  33. Gut Pathog. 2015 Oct 19;7:28 - PubMed
  34. J Appl Microbiol. 2013 Aug;115(2):583-90 - PubMed

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