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Suzuki O, Abe M, Hashimoto Y. Caspase-dependent drug-induced apoptosis is regulated by cell surface sialylation in human B-cell lymphoma. Oncol Lett. 2015;10(2):687-690doi: 10.3892/ol.2015.3320.
Suzuki, O., Abe, M., & Hashimoto, Y. (2015). Caspase-dependent drug-induced apoptosis is regulated by cell surface sialylation in human B-cell lymphoma. Oncology letters, 10(2), 687-690. https://doi.org/10.3892/ol.2015.3320
Suzuki, Osamu, et al. "Caspase-dependent drug-induced apoptosis is regulated by cell surface sialylation in human B-cell lymphoma." Oncology letters vol. 10,2 (2015): 687-690. doi: https://doi.org/10.3892/ol.2015.3320
Suzuki O, Abe M, Hashimoto Y. Caspase-dependent drug-induced apoptosis is regulated by cell surface sialylation in human B-cell lymphoma. Oncol Lett. 2015 Aug;10(2):687-690. doi: 10.3892/ol.2015.3320. Epub 2015 Jun 04. PMID: 26622554; PMCID: PMC4508975.
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Oncol Lett. 2015 Aug;10(2):687-690. doi: 10.3892/ol.2015.3320. Epub 2015 Jun 04.

Caspase-dependent drug-induced apoptosis is regulated by cell surface sialylation in human B-cell lymphoma.

Oncology letters

Osamu Suzuki, Masafumi Abe, Yuko Hashimoto

Affiliations

  1. Department of Diagnostic Pathology, School of Medicine, Fukushima Medical University, Fukushima 960-1295, Japan.

PMID: 26622554 PMCID: PMC4508975 DOI: 10.3892/ol.2015.3320

Abstract

The important role of sialic acid in various biological phenomena is well-established. In order to further clarify the role of sialic acid in cell death induced by various stimuli, the present study compared the cell survival of the HBL-2 human diffuse large B-cell lymphoma cell line upon anticancer drug-induced cell death, with or without neuraminidase pretreatment: Cell survival was assessed using flow cytometry. Upon treatment with doxorubicin or etoposide, the HBL-2 cell viability decreased. In etoposide-induced cell death, the HBL-2 cells demonstrated nuclear fragmentation, which was consistent with morphologically apoptotic cells. In addition, a higher decrease in the cell viability of etoposide-treated HBL-2 cells was observed in cells pretreated with neuraminidase compared with cells that were not pretreated. Furthermore, the caspase-3, caspase-8 and caspase-9 activities in etoposide-induced apoptosis demonstrated a greater increase upon neuraminidase pretreatment compared with no neuraminidase pretreatment. In conclusion, cell surface sialylation appears to protect lymphoma cells from anticancer drug-induced apoptosis.

Keywords: drug-induced apoptosis; human malignant lymphoma; sialic acid

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