Display options
Cite
Dicksved J, Jansson JK, Lindberg JE. Fecal microbiome of growing pigs fed a cereal based diet including chicory (Cichorium intybus L.) or ribwort (Plantago lanceolata L.) forage. J Anim Sci Biotechnol. 2015;6:53doi: 10.1186/s40104-015-0054-8.
Dicksved, J., Jansson, J. K., & Lindberg, J. E. (2015). Fecal microbiome of growing pigs fed a cereal based diet including chicory (Cichorium intybus L.) or ribwort (Plantago lanceolata L.) forage. Journal of animal science and biotechnology, 653. https://doi.org/10.1186/s40104-015-0054-8
Dicksved, Johan, et al. "Fecal microbiome of growing pigs fed a cereal based diet including chicory (Cichorium intybus L.) or ribwort (Plantago lanceolata L.) forage." Journal of animal science and biotechnology vol. 6 (2015): 53. doi: https://doi.org/10.1186/s40104-015-0054-8
Dicksved J, Jansson JK, Lindberg JE. Fecal microbiome of growing pigs fed a cereal based diet including chicory (Cichorium intybus L.) or ribwort (Plantago lanceolata L.) forage. J Anim Sci Biotechnol. 2015 Dec 18;6:53. doi: 10.1186/s40104-015-0054-8. eCollection 2015. PMID: 26688727; PMCID: PMC4683726.
Copy Download .nbib Format: NLM
Share it on

J Anim Sci Biotechnol. 2015 Dec 18;6:53. doi: 10.1186/s40104-015-0054-8. eCollection 2015.

Fecal microbiome of growing pigs fed a cereal based diet including chicory (Cichorium intybus L.) or ribwort (Plantago lanceolata L.) forage.

Journal of animal science and biotechnology

Johan Dicksved, Janet K Jansson, Jan Erik Lindberg

Affiliations

  1. Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, P.O. Box 7024, SE75007 Uppsala, Sweden ; Department of Microbiology, Swedish University of Agricultural Sciences, P.O. Box 7025, SE75007 Uppsala, Sweden.
  2. Division of Biology Earth and Biological Sciences, Pacific Northwest National Laboratories, P.O. Box 999, MSIN J4-18, WA99352 Richland, WA USA.
  3. Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, P.O. Box 7024, SE75007 Uppsala, Sweden.

PMID: 26688727 PMCID: PMC4683726 DOI: 10.1186/s40104-015-0054-8

Abstract

BACKGROUND: The purpose of this study was to investigate how inclusion of chicory forage or ribwort forage in a cereal-based diet influenced the fecal microbial community (microbiome) in newly weaned (35 days of age) piglets. The piglets were fed a cereal-based diet without (B) and with inclusion (80 and 160 g/kg air-dry forage) of vegetative shoots of chicory (C) and leaves of ribwort (R) forage in a 35-day growth trial. Fecal samples were collected at the start (D0), 17 (D17) and 35 (D35) days after weaning and profiles of the microbial consortia were generated using terminal restriction fragment length polymorphism (T-RFLP). 454-FLX pyrosequencing of 16S rRNA gene amplicons was used to analyze the microbial composition in a subset of the samples already analyzed with T-RFLP.

RESULTS: The microbial clustering pattern was primarily dependent on age of the pigs, but diet effects could also be observed. Lactobacilli and enterobacteria were more abundant at D0, whereas the genera Streptococcus, Treponema, Clostridium, Clostridiaceae1 and Coprococcus were present in higher abundances at D35. Pigs fed ribwort had an increased abundance of sequences classified as Treponema and a reduction in lactobacilli. However, the abundance of Prevotellaceae increased with age in on both the chicory and the ribwort diet. Moreover, there were significant correlations between the abundance of Bacteroides and the digested amount of galactose, uronic acids and total non-starch polysaccharides, and between the abundance of Bacteroidales and the digested amount of xylose.

CONCLUSION: This study demonstrated that both chicory and ribwort inclusion in the diet of newly weaned pigs influenced the composition of the fecal microbiota and that digestion of specific dietary components was correlated with species composition of the microbiota. Moreover, this study showed that the gut will be exposed to a dramatic shift in the microbial community structure several weeks after weaning.

Keywords: 16S; Amplicon sequencing; Chicory; Microbiome; Ribwort; Uronic acid; Weaning

References

  1. Appl Environ Microbiol. 2007 Apr;73(7):2284-9 - PubMed
  2. FEMS Microbiol Ecol. 2014 Dec;90(3):791-801 - PubMed
  3. J Nutr. 1995 Jun;125(6):1401-12 - PubMed
  4. Cell Host Microbe. 2015 Jun 10;17(6):852 - PubMed
  5. Proc Natl Acad Sci U S A. 2010 Aug 17;107(33):14691-6 - PubMed
  6. Sci Rep. 2015 Apr 21;5:9938 - PubMed
  7. PLoS One. 2015 Feb 17;10(2):e0117441 - PubMed
  8. Vet Microbiol. 2011 Nov 21;153(1-2):124-33 - PubMed
  9. PLoS One. 2008 Jul 30;3(7):e2836 - PubMed
  10. Appl Environ Microbiol. 1977 Nov;34(5):529-33 - PubMed
  11. Appl Environ Microbiol. 2012 Jun;78(12):4102-9 - PubMed
  12. Environ Microbiol. 2006 Jul;8(7):1191-9 - PubMed
  13. Appl Environ Microbiol. 2004 Jul;70(7):3821-30 - PubMed
  14. FEMS Microbiol Ecol. 2008 Dec;66(3):546-55 - PubMed
  15. Animal. 2012 Jul;6(7):1077-85 - PubMed
  16. Ann N Y Acad Sci. 2008 Mar;1125:280-8 - PubMed
  17. Nucleic Acids Res. 1989 Oct 11;17(19):7843-53 - PubMed
  18. Animal. 2014 Nov;8(11):1777-87 - PubMed
  19. Appl Environ Microbiol. 2001 Jun;67(6):2766-74 - PubMed
  20. Appl Environ Microbiol. 2012 Jan;78(2):420-8 - PubMed
  21. Animal. 2011 Feb;5(4):558-64 - PubMed
  22. Appl Environ Microbiol. 2002 Feb;68(2):673-90 - PubMed
  23. Appl Environ Microbiol. 1977 Feb;33(2):319-22 - PubMed
  24. J Agric Food Chem. 2002 Apr 24;50(9):2514-8 - PubMed
  25. Cell Rep. 2015 Apr 28;11(4):527-38 - PubMed
  26. Appl Environ Microbiol. 1993 Mar;59(3):695-700 - PubMed

Publication Types