Nutr Diabetes. 2015 Dec 14;5:e188. doi: 10.1038/nutd.2015.40.
A comparison of effects of lard and hydrogenated vegetable shortening on the development of high-fat diet-induced obesity in rats.
Nutrition & diabetes
R Kubant, A N Poon, D Sánchez-Hernández, A F Domenichiello, P S P Huot, E Pannia, C E Cho, S Hunschede, R P Bazinet, G H Anderson
Affiliations
Affiliations
- Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada.
- Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada.
PMID: 26657014
PMCID: PMC4735054 DOI: 10.1038/nutd.2015.40
Abstract
BACKGROUND: Obesity is associated with increased consumption and preference for dietary fat. Experimental models of fat-induced obesity use either lard or vegetable shortening. Yet, there are no direct comparisons of these commonly used fat sources, or the influence of their fatty acid composition, on the development of diet-induced obesity.
OBJECTIVE: To compare the effects of lard and hydrogenated vegetable-shortening diets, which differ in their fatty acid composition, on weight gain and the development of obesity and insulin resistance in rats.
METHODS AND DESIGN: Male Wistar rats were fed ad libitum for 14 weeks high-fat diets containing either (1) high vegetable fat (HVF, 60 kcal% from vegetable shortening) or (2) high lard fat (HLF, 60 kcal% from lard). Rats fed normal-fat (NF, 16 kcal% from vegetable shortening) diet served as control. Body weight, food intake, adipose tissue mass, serum 25[OH]D3, glucose, insulin and fatty acid composition of diets were measured.
RESULTS: Rats fed either of the two high-fat diets had higher energy intake, weight gain and fat accretion than rats fed normal-fat diet. However, rats fed the HLF diet consumed more calories and gained more weight and body fat with greater increases of 32% in total (158.5±8.2 vs 120.2±6.6 g, P<0.05), 30% in visceral (104.4±5.2 vs 80.3±4.2 g, P<0.05) and 36% in subcutaneous fat mass (54.1±3.6 vs 39.9±3.1 g, P<0.05), compared with rats fed the HVF diet. Higher visceral adiposity was positively correlated with serum insulin (r=0.376, P<0.05) and homeostatic model assessment insulin resistance (r=0.391, P<0.05).
CONCLUSION: We conclude that lard-based high-fat diets accentuate the increase in weight gain and the development of obesity and insulin resistance more than hydrogenated vegetable-shortening diets. These results further point to the importance of standardizing fatty acid composition and type of fat used in determining outcomes of consuming high-fat diets.
References
- PLoS One. 2013 Jul 30;8(7):e68436 - PubMed
- Int J Obes (Lond). 2012 Mar;36(3):387-96 - PubMed
- J Intern Med. 2010 May;267(5):462-72 - PubMed
- J Nutr Sci Vitaminol (Tokyo). 2007 Feb;53(1):1-4 - PubMed
- J Nutr. 1995 Feb;125(2):229-37 - PubMed
- Arteriosclerosis. 1990 Jul-Aug;10(4):493-6 - PubMed
- AJR Am J Roentgenol. 2005 Feb;184(2):658-62 - PubMed
- Metabolism. 2008 Feb;57(2):183-91 - PubMed
- Am J Clin Nutr. 2007 Sep;86(3):s867-71 - PubMed
- J Lipid Res. 2014 Jan;55(1):62-74 - PubMed
- Am J Clin Nutr. 2011 May;93(5):950-62 - PubMed
- Eur J Endocrinol. 2008 Dec;159(6):675-84 - PubMed
- Stroke. 2000 Nov;31(11):2751-66 - PubMed
- Int J Vitam Nutr Res. 2009 Mar;79(2):71-8 - PubMed
- Clin Nutr. 2015 Oct;34(5):904-10 - PubMed
- Am J Clin Nutr. 2000 Sep;72(3):690-3 - PubMed
- Mol Nutr Food Res. 2007 Oct;51(10):1215-24 - PubMed
- Am J Physiol Endocrinol Metab. 2008 Nov;295(5):E1269-76 - PubMed
- Atherosclerosis. 2014 Jun;234(2):320-8 - PubMed
- J Oleo Sci. 2013;62(2):57-64 - PubMed
- J Nutr. 1958 Feb 10;64(2):241-9 - PubMed
- PLoS One. 2014 Mar 24;9(3):e92753 - PubMed
- Endocrinology. 2004 May;145(5):2174-85 - PubMed
- Acta Med Scand. 1987;222(4):361-7 - PubMed
- Obesity (Silver Spring). 2007 Apr;15(4):798-808 - PubMed
- Prog Lipid Res. 2006 May;45(3):203-36 - PubMed
- Lipids. 2006 Jun;41(6):535-41 - PubMed
- Obesity (Silver Spring). 2007 Jul;15(7):1675-84 - PubMed
- Clin Nutr. 2015 Oct;34(5):997-1009 - PubMed
- Inflammopharmacology. 2008 Oct;16(5):216-8 - PubMed
- J Am Diet Assoc. 1997 Jul;97(7 Suppl):S58-62 - PubMed
- J Nutr. 1993 Nov;123(11):1939-51 - PubMed
- Free Radic Biol Med. 1994 Oct;17(4):367-72 - PubMed
- Obesity (Silver Spring). 2012 Oct;20(10 ):1984-94 - PubMed
- Nutr Metab (Lond). 2006 Jan 20;3:8 - PubMed
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