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Song H, Han YZ, Cai GH, et al. The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell. Int J Clin Exp Med. 2015;8(9):14906-15
Song, H., Han, Y. Z., Cai, G. H., Tang, F. S., Yang, Z. H., Ao, D. S., & Zhou, A. (2015). The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell. International journal of clinical and experimental medicine, 8(9), 14906-15.
Song, Hong, et al. "The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell." International journal of clinical and experimental medicine vol. 8,9 (2015): 14906-15.
Song H, Han YZ, Cai GH, Tang FS, Yang ZH, Ao DS, Zhou A. The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell. Int J Clin Exp Med. 2015 Sep 15;8(9):14906-15. eCollection 2015. PMID: 26628972; PMCID: PMC4658861.
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Int J Clin Exp Med. 2015 Sep 15;8(9):14906-15. eCollection 2015.

The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell.

International journal of clinical and experimental medicine

Hong Song, Yun-Zhu Han, Guo-Hui Cai, Fu-Shan Tang, Ze-Hong Yang, Di-Shu Ao, An Zhou

Affiliations

  1. College of Basic Medicine, Zunyi Medical University No. 201 Dalian Road, Huichuan District, Zunyi 563003, China.
  2. Biomolecular Medicine Lab, West China School of Preclinical Medicine and Forensic Medicine, Sichuan University Chengdu, China.

PMID: 26628972 PMCID: PMC4658861

Abstract

The aim of this study will provide a self-assembling peptide (RADA16-I) -derived hydrogel as a tool for investigation the malignant phenotype of human hepatocellular carcinoma cell. Characteristic analysis indicated that the peptide consists of a well-defined secondary structure and self-assembly property. Our results showed that these cells cultured in RADA16-I hydrogels showed a spindle-shaped phenotype with irregular and radial nuclei. Immunohistochemical results showed that the expression of fibronectin in hepatocellular carcinoma cells is positive cultured in RADA16-I hydrogels, and the expression levels of laminin are weakly positive. DNA contents cultured in RADA16-I hydrogel gradually increased up to Day 9. The expression levels of VEGFA, EGF and FGF2 in three hydrogels showed no statistically significant differences (P > 0.05), and the expression levels of IGF-1 in RADA16-I and collagen-I were significantly lower than those of in the Matrigel hydrogel (P ≤ 0.05). These findings suggested that the RADA16-I will help to provide a better physiological substrate for hepatocellular carcinoma cell culture, may serve as an ideal model for cancer biology research of tumorigenesis, growth, local invasion, and metastasis.

Keywords: Self-assembling peptide; hepatocellular carcinoma cell; hydrogel; malignant phenotype; three-dimensional culture

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