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Karami Kheirabad M, Namavar Jahromi B, Tamadon A, et al. Expression of Melanocortin-4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress. Int J Mol Cell Med. 2015;4(3):182-7
Karami Kheirabad, M., Namavar Jahromi, B., Tamadon, A., Ramezani, A., Ahmadloo, S., Sabet Sarvestan, F., & Koohi-Hosseinabadi, O. (2015). Expression of Melanocortin-4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress. International journal of molecular and cellular medicine, 4(3), 182-7.
Karami Kheirabad, Maryam, et al. "Expression of Melanocortin-4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress." International journal of molecular and cellular medicine vol. 4,3 (2015): 182-7.
Karami Kheirabad M, Namavar Jahromi B, Tamadon A, Ramezani A, Ahmadloo S, Sabet Sarvestan F, Koohi-Hosseinabadi O. Expression of Melanocortin-4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress. Int J Mol Cell Med. 2015;4(3):182-7. PMID: 26629487; PMCID: PMC4644530.
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Int J Mol Cell Med. 2015;4(3):182-7.

Expression of Melanocortin-4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress.

International journal of molecular and cellular medicine

Maryam Karami Kheirabad, Bahia Namavar Jahromi, Amin Tamadon, Amin Ramezani, Somayeh Ahmadloo, Fatemeh Sabet Sarvestan, Omid Koohi-Hosseinabadi

Affiliations

  1. Department of Basic Sciences, Azad University, Gachsaran Branch, Gachsaran, Iran.
  2. Infertility Research Center, Department of OB-GYN, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
  3. Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
  4. Department of Medical Biotechnology, School of Advanced Medical Sciences and Technology, Shiraz University of Medical Sciences, Shiraz, Iran. ; Institute of Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran.
  5. Laboratory Animal Center, Shiraz University of Medical Sciences, Shiraz, Iran.

PMID: 26629487 PMCID: PMC4644530

Abstract

The effects of chronic stress and glucocorticoids receptor antagonist (RU486) on expression of melanocortin 4 receptor (MC4R) mRNA in arcuate nucleus (ARC) of male rats were evaluated. In this study, adult male Sprague Dawley rats were placed into four groups (n=6/group); stress, RU486, stress/RU486, and control groups. In stress group, the rats were restrained, 1 h/day, for 12 days. In RU486 group, the rats were injected RU486 for 12 days. In stress/RU486 group, the rats were injected RU486 1 h before the stress process for 12 days. Relative expression of MC4R mRNA was determined using real-time PCR. Relative expression of MC4R mRNA in the stress group was higher than that of the control rats (P<0.05). Relative expressions of MC4R mRNA were not different between the stress, RU486 and stress/RU486 groups (P>0.05). Chronic restraint stress causes increase in mRNA expression of MC4R in ARC and blockade of glucocorticoid receptors has no effect on this up-regulation.

Keywords: Chronic stress; hypothalamus; melanocortin 4 receptor (MC4R); rats

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