Display options
Cite
Eram MS, Sarafuddin B, Gong F, et al. Optimization of expression and properties of the recombinant acetohydroxyacid synthase of Thermotoga maritima. Data Brief. 2015;5:489-97doi: 10.1016/j.dib.2015.09.018.
Eram, M. S., Sarafuddin, B., Gong, F., & Ma, K. (2015). Optimization of expression and properties of the recombinant acetohydroxyacid synthase of Thermotoga maritima. Data in brief, 5489-97. https://doi.org/10.1016/j.dib.2015.09.018
Eram, Mohammad S, et al. "Optimization of expression and properties of the recombinant acetohydroxyacid synthase of Thermotoga maritima." Data in brief vol. 5 (2015): 489-97. doi: https://doi.org/10.1016/j.dib.2015.09.018
Eram MS, Sarafuddin B, Gong F, Ma K. Optimization of expression and properties of the recombinant acetohydroxyacid synthase of Thermotoga maritima. Data Brief. 2015 Oct 03;5:489-97. doi: 10.1016/j.dib.2015.09.018. eCollection 2015 Dec. PMID: 26629492; PMCID: PMC4631844.
Copy Download .nbib Format: NLM
Share it on

Data Brief. 2015 Oct 03;5:489-97. doi: 10.1016/j.dib.2015.09.018. eCollection 2015 Dec.

Optimization of expression and properties of the recombinant acetohydroxyacid synthase of Thermotoga maritima.

Data in brief

Mohammad S Eram, Benozir Sarafuddin, Frank Gong, Kesen Ma

Affiliations

  1. Department of Biology, University of Waterloo, Waterloo, Ontario, Canada.

PMID: 26629492 PMCID: PMC4631844 DOI: 10.1016/j.dib.2015.09.018

Abstract

The data provide additional support of the characterization of the biophysical and biochemical properties of the enzyme acetohydroxyacid synthase from the hyperthermophilic bacterium Thermotoga maritima (Eram et al., 2015) [1]. The genes encoding the enzyme subunits have been cloned and expressed in the mesophilic host Escherichia coli. Detailed data include information about the optimization of the expression conditions, biophysical properties of the enzyme and reconstitution of the holoenzyme from individually expressed and purified subunits.

Keywords: Acetohydroxyacid synthase; Heat-treatment; Hyperthermophiles; Thermotoga

References

  1. J Biol Chem. 1999 Jul 23;274(30):21023-8 - PubMed
  2. J Mol Biol. 1999 Sep 3;291(5):1135-46 - PubMed
  3. J Mol Biol. 2000 Jan 14;295(2):279-88 - PubMed
  4. Gene. 2002 Jan 23;283(1-2):107-15 - PubMed
  5. FEMS Microbiol Lett. 2002 Jun 18;212(1):121-6 - PubMed
  6. Extremophiles. 2005 Oct;9(5):399-406 - PubMed
  7. Extremophiles. 2006 Jun;10(3):221-7 - PubMed
  8. Microb Cell Fact. 2009 Jun 04;8:30 - PubMed
  9. Syst Appl Microbiol. 2011 Dec;34(8):581-5 - PubMed
  10. J Bacteriol. 1990 Aug;172(8):4329-38 - PubMed
  11. Extremophiles. 2012 May;16(3):387-93 - PubMed
  12. J Biochem. 2015 Dec;158(6):459-66 - PubMed
  13. Biochem Biophys Rep. 2015 Aug 28;4:89-97 - PubMed
  14. Microbiol Rev. 1979 Jun;43(2):260-96 - PubMed
  15. Appl Environ Microbiol. 1995 May;61(5):1867-75 - PubMed
  16. J Biol Chem. 1995 Mar 17;270(11):5748-55 - PubMed
  17. Eur J Biochem. 1993 May 15;214(1):43-50 - PubMed
  18. J Bacteriol. 1996 Jan;178(2):484-9 - PubMed
  19. J Mol Biol. 1998 Jul 17;280(3):525-33 - PubMed

Publication Types