Display options
Cite
Mahmood MA, Hasan MR, Khan UJ, et al. One-step tumor detection from dynamic morphology tracking on aptamer-grafted surfaces. Technology (Singap World Sci). 2015;3(4):194-200doi: 10.1142/S2339547815500089.
Mahmood, M. A., Hasan, M. R., Khan, U. J., Allen, P. B., Kim, Y. T., Ellington, A. D., & Iqbal, S. M. (2015). One-step tumor detection from dynamic morphology tracking on aptamer-grafted surfaces. Technology, 3(4), 194-200. https://doi.org/10.1142/S2339547815500089
Mahmood, Mohammed Arif I, et al. "One-step tumor detection from dynamic morphology tracking on aptamer-grafted surfaces." Technology vol. 3,4 (2015): 194-200. doi: https://doi.org/10.1142/S2339547815500089
Mahmood MA, Hasan MR, Khan UJ, Allen PB, Kim YT, Ellington AD, Iqbal SM. One-step tumor detection from dynamic morphology tracking on aptamer-grafted surfaces. Technology (Singap World Sci). 2015 Dec;3(4):194-200. doi: 10.1142/S2339547815500089. Epub 2015 Nov 16. PMID: 26753172; PMCID: PMC4703374.
Copy Download .nbib Format: NLM
Share it on

Technology (Singap World Sci). 2015 Dec;3(4):194-200. doi: 10.1142/S2339547815500089. Epub 2015 Nov 16.

One-step tumor detection from dynamic morphology tracking on aptamer-grafted surfaces.

Technology

Mohammed Arif I Mahmood, Mohammad Raziul Hasan, Umair J M Khan, Peter B Allen, Young-Tae Kim, Andrew D Ellington, Samir M Iqbal

Affiliations

  1. Nano-Bio Lab, University of Texas at Arlington, TX 76019, USA; Department of Electrical Engineering, University of Texas at Arlington, TX 76010, USA; Nanotechnology Research Center, University of Texas at Arlington, TX 76019, USA.
  2. Nano-Bio Lab, University of Texas at Arlington, TX 76019, USA; Nanotechnology Research Center, University of Texas at Arlington, TX 76019, USA; Department of Biology, University of Texas at Arlington, TX 76019, USA.
  3. Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA; Now at Department of Chemistry, University of Idaho, Moscow, ID 83844-2343, USA.
  4. Nanotechnology Research Center, University of Texas at Arlington, TX 76019, USA; Department of Bioengineering, University of Texas at Arlington, TX 76011, USA; Department of Urology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA.
  5. Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA.
  6. Nano-Bio Lab, University of Texas at Arlington, TX 76019, USA; Department of Electrical Engineering, University of Texas at Arlington, TX 76010, USA; Nanotechnology Research Center, University of Texas at Arlington, TX 76019, USA; Department of Biology, University of Texas at Arlington, TX 76019, USA; Department of Urology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA.

PMID: 26753172 PMCID: PMC4703374 DOI: 10.1142/S2339547815500089

Abstract

In this paper, we report a one-step tumor cell detection approach based on the dynamic morphological behavior tracking of cancer cells on a ligand modified surface. Every cell on the surface was tracked in real time for several minutes immediately after seeding until these were finally attached. Cancer cells were found to be very active in the aptamer microenvironment, changing their shapes rapidly from spherical to semi-elliptical, with much flatter spread and extending pseudopods at regular intervals. When incubated on a functionalized surface, the balancing forces between cell surface molecules and the surface-bound aptamers, together with the flexibility of the membranes, caused cells to show these distinct dynamic activities and variations in their morphologies. On the other hand, healthy cells remained distinguishingly inactive on the surface over the same period. The quantitative image analysis of cell morphologies provided feature vectors that were statistically distinct between normal and cancer cells.

Keywords: Cancer Cell Morphology; Cell Imaging; Early Cancer Diagnosis; Human Glioblastoma (hGBM); Temporal Image Processing

References

  1. Clin Cancer Res. 2006 Sep 1;12(17):5064-73 - PubMed
  2. Lab Chip. 2012 Nov 21;12 (22):4693-701 - PubMed
  3. Nat Nanotechnol. 2007 Dec;2(12):780-3 - PubMed
  4. Anal Biochem. 2001 Jul 15;294(2):126-31 - PubMed
  5. Int J Biol Sci. 2007 Jun 01;3(5):303-17 - PubMed
  6. Anal Chem. 2008 Feb 1;80(3):567-72 - PubMed
  7. Cancer Res. 1983 Sep;43(9):3982-97 - PubMed
  8. Proc Natl Acad Sci U S A. 1992 May 15;89(10):4309-13 - PubMed
  9. Mol Cell Endocrinol. 1983 Jul;31(1):1-19 - PubMed
  10. Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1591-5 - PubMed
  11. Chem Soc Rev. 2015 Mar 7;44(5):1240-56 - PubMed
  12. Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3745-9 - PubMed
  13. Int J Radiat Oncol Biol Phys. 1996 Mar 1;34(4):809-15 - PubMed
  14. Nat Rev Cancer. 2003 Jan;3(1):11-22 - PubMed
  15. Int J Radiat Oncol Biol Phys. 2001 Oct 1;51(2):410-8 - PubMed
  16. Nature. 2007 Dec 20;450(7173):1235-9 - PubMed
  17. Cancer Res. 1981 Dec;41(12 Pt 1):5076-81 - PubMed
  18. Biosens Bioelectron. 2007 Jun 15;22(12):3126-31 - PubMed
  19. Nat Nanotechnol. 2007 Apr;2(4):243-8 - PubMed
  20. Cancer Res. 2010 Nov 15;70(22):9371-80 - PubMed
  21. Cancer. 2012 Feb 15;118(4):1145-54 - PubMed
  22. Cancer Biochem Biophys. 1992 Oct;13(1):57-64 - PubMed
  23. Electrophoresis. 2002 Jul;23(13):1973-83 - PubMed
  24. PLoS One. 2011;6(6):e20299 - PubMed
  25. Anal Bioanal Chem. 2010 Aug;397(8):3249-67 - PubMed
  26. Annu Rev Biochem. 1979;48:193-216 - PubMed
  27. Mol Oncol. 2007 Jun;1(1):84-96 - PubMed

Publication Types

Grant support