Display options
Cite
Beyerlein KR, Adriano L, Heymann M, et al. Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery. Rev Sci Instrum. 2015;86(12):125104doi: 10.1063/1.4936843.
Beyerlein, K. R., Adriano, L., Heymann, M., Kirian, R., Knoška, J., Wilde, F., Chapman, H. N., & Bajt, S. (2015). Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery. The Review of scientific instruments, 86(12), 125104. https://doi.org/10.1063/1.4936843
Beyerlein, K R, et al. "Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery." The Review of scientific instruments vol. 86,12 (2015): 125104. doi: https://doi.org/10.1063/1.4936843
Beyerlein KR, Adriano L, Heymann M, Kirian R, Knoška J, Wilde F, Chapman HN, Bajt S. Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery. Rev Sci Instrum. 2015 Dec;86(12):125104. doi: 10.1063/1.4936843. PMID: 26724070.
Copy Download .nbib Format: NLM
Share it on

Rev Sci Instrum. 2015 Dec;86(12):125104. doi: 10.1063/1.4936843.

Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery.

The Review of scientific instruments

K R Beyerlein, L Adriano, M Heymann, R Kirian, J Knoška, F Wilde, H N Chapman, S Bajt

Affiliations

  1. Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestra?e 85, 22607 Hamburg, Germany.
  2. Photon Science, Deutsches Elektronen-Synchrotron, Notkestra?e 85, 22607 Hamburg, Germany.
  3. Department of Physics, University of Hamburg, Luruper Chaussee 149, 22607 Hamburg, Germany.
  4. Helmholtz-Zentrum Geesthacht, Max-Planck-Straße 1, 21502 Geesthacht, Germany.

PMID: 26724070 DOI: 10.1063/1.4936843

Abstract

Serial femtosecond crystallography (SFX) using X-ray Free-Electron Lasers (XFELs) allows for room temperature protein structure determination without evidence of conventional radiation damage. In this method, a liquid suspension of protein microcrystals can be delivered to the X-ray beam in vacuum as a micro-jet, which replenishes the crystals at a rate that exceeds the current XFEL pulse repetition rate. Gas dynamic virtual nozzles produce the required micrometer-sized streams by the focusing action of a coaxial sheath gas and have been shown to be effective for SFX experiments. Here, we describe the design and characterization of such nozzles assembled from ceramic micro-injection molded outer gas-focusing capillaries. Trends of the emitted jet diameter and jet length as a function of supplied liquid and gas flow rates are measured by a fast imaging system. The observed trends are explained by derived relationships considering choked gas flow and liquid flow conservation. Finally, the performance of these nozzles in a SFX experiment is presented, including an analysis of the observed background.

Publication Types