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Chen K, Dai W, Wang F, et al. Inhibitive effects of 15-deoxy-Δ(12),(14)-prostaglandin J2 on hepatoma-cell proliferation through reactive oxygen species-mediated apoptosis. Onco Targets Ther. 2015;8:3585-93doi: 10.2147/OTT.S92832.
Chen, K., Dai, W., Wang, F., Xia, Y., Li, J., Li, S., Liu, T., Zhang, R., Wang, J., Lu, W., Zhou, Y., Yin, Q., Zheng, Y., Abudumijiti, H., Chen, R., Lu, J., Zhou, Y., & Guo, C. (2015). Inhibitive effects of 15-deoxy-Δ(12),(14)-prostaglandin J2 on hepatoma-cell proliferation through reactive oxygen species-mediated apoptosis. OncoTargets and therapy, 83585-93. https://doi.org/10.2147/OTT.S92832
Chen, Kan, et al. "Inhibitive effects of 15-deoxy-Δ(12),(14)-prostaglandin J2 on hepatoma-cell proliferation through reactive oxygen species-mediated apoptosis." OncoTargets and therapy vol. 8 (2015): 3585-93. doi: https://doi.org/10.2147/OTT.S92832
Chen K, Dai W, Wang F, Xia Y, Li J, Li S, Liu T, Zhang R, Wang J, Lu W, Zhou Y, Yin Q, Zheng Y, Abudumijiti H, Chen R, Lu J, Zhou Y, Guo C. Inhibitive effects of 15-deoxy-Δ(12),(14)-prostaglandin J2 on hepatoma-cell proliferation through reactive oxygen species-mediated apoptosis. Onco Targets Ther. 2015 Dec 01;8:3585-93. doi: 10.2147/OTT.S92832. eCollection 2015. PMID: 26664142; PMCID: PMC4671813.
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Onco Targets Ther. 2015 Dec 01;8:3585-93. doi: 10.2147/OTT.S92832. eCollection 2015.

Inhibitive effects of 15-deoxy-Δ(12),(14)-prostaglandin J2 on hepatoma-cell proliferation through reactive oxygen species-mediated apoptosis.

OncoTargets and therapy

Kan Chen, Weiqi Dai, Fan Wang, Yujing Xia, Jingjing Li, Sainan Li, Tong Liu, Rong Zhang, Jianrong Wang, Wenxia Lu, Yuqing Zhou, Qin Yin, Yuanyuan Zheng, Huerxidan Abudumijiti, Rongxia Chen, Jie Lu, Yingqun Zhou, Chuanyong Guo

Affiliations

  1. Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, People's Republic of China.
  2. Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, First Clinical Medical College of Nanjing Medical University, Nanjing, People's Republic of China.
  3. Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China.

PMID: 26664142 PMCID: PMC4671813 DOI: 10.2147/OTT.S92832

Abstract

OBJECTIVE: 15-Deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) induces reactive oxygen species (ROS)-mediated apoptosis in many malignant cells, which has not been studied in hepatoma cells. In this study, we investigated whether 15d-PGJ2 induced apoptosis in hepatocellular carcinoma (HCC) associated with ROS.

MATERIALS AND METHODS: The LM3, SMMC-7721, and Huh-7 HCC cell lines were treated with 15d-PGJ2 (5-40 μM) for 24, 48, and 72 hours. Cholecystokinin 8 was used to detect the cytotoxicity of 15d-PGJ2. Flow cytometry, Hoechst staining, and Western blotting were used to analyze apoptosis. ROS were combined with the fluorescent probe dihydroethidium and then observed by fluorescence microscopy and flow cytometry. Activation of JNK and expression of Akt were detected by Western blotting.

RESULTS: 15d-PGJ2 inhibited HCC cell proliferation and induced apoptosis in a dose- and time-dependent manner. Apoptosis was mainly induced via an intrinsic pathway and was ROS-dependent, and was alleviated by ROS scavengers. ROS induced JNK activation and Akt downregulation in HCC cells.

CONCLUSION: 15d-PGJ2 induced ROS in HCC cell lines, and inhibition of cell growth and apoptosis were partly ROS-dependent.

Keywords: 15-deoxy-Δ12,14-prostaglandin J2; JNK; ROS; apoptosis; hepatoma cell

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