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Laidò G, Panio G, Marone D, et al. Identification of New Resistance Loci to African Stem Rust Race TTKSK in Tetraploid Wheats Based on Linkage and Genome-Wide Association Mapping. Front Plant Sci. 2015;6:1033doi: 10.3389/fpls.2015.01033.
Laidò, G., Panio, G., Marone, D., Russo, M. A., Ficco, D. B., Giovanniello, V., Cattivelli, L., Steffenson, B., de Vita, P., & Mastrangelo, A. M. (2015). Identification of New Resistance Loci to African Stem Rust Race TTKSK in Tetraploid Wheats Based on Linkage and Genome-Wide Association Mapping. Frontiers in plant science, 61033. https://doi.org/10.3389/fpls.2015.01033
Laidò, Giovanni, et al. "Identification of New Resistance Loci to African Stem Rust Race TTKSK in Tetraploid Wheats Based on Linkage and Genome-Wide Association Mapping." Frontiers in plant science vol. 6 (2015): 1033. doi: https://doi.org/10.3389/fpls.2015.01033
Laidò G, Panio G, Marone D, Russo MA, Ficco DB, Giovanniello V, Cattivelli L, Steffenson B, de Vita P, Mastrangelo AM. Identification of New Resistance Loci to African Stem Rust Race TTKSK in Tetraploid Wheats Based on Linkage and Genome-Wide Association Mapping. Front Plant Sci. 2015 Dec 09;6:1033. doi: 10.3389/fpls.2015.01033. eCollection 2015. PMID: 26697025; PMCID: PMC4673868.
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Front Plant Sci. 2015 Dec 09;6:1033. doi: 10.3389/fpls.2015.01033. eCollection 2015.

Identification of New Resistance Loci to African Stem Rust Race TTKSK in Tetraploid Wheats Based on Linkage and Genome-Wide Association Mapping.

Frontiers in plant science

Giovanni Laidò, Giosuè Panio, Daniela Marone, Maria A Russo, Donatella B M Ficco, Valentina Giovanniello, Luigi Cattivelli, Brian Steffenson, Pasquale de Vita, Anna M Mastrangelo

Affiliations

  1. Cereal Research Centre, Council for Agricultural Research and Economics Foggia, Italy.
  2. Cereal Research Centre, Council for Agricultural Research and Economics Foggia, Italy ; Genomics Research Centre, Council for Agricultural Research and Economics Fiorenzuola d'Arda, Italy.
  3. Department of Plant Pathology, University of Minnesota Twin Cities Minneapolis, MN, USA.

PMID: 26697025 PMCID: PMC4673868 DOI: 10.3389/fpls.2015.01033

Abstract

Stem rust, caused by Puccinia graminis Pers. f. sp. tritici Eriks. and E. Henn. (Pgt), is one of the most destructive diseases of wheat. Races of the pathogen in the "Ug99 lineage" are of international concern due to their virulence for widely used stem rust resistance genes and their spread throughout Africa. Disease resistant cultivars provide one of the best means for controlling stem rust. To identify quantitative trait loci (QTL) conferring resistance to African stem rust race TTKSK at the seedling stage, we evaluated an association mapping (AM) panel consisting of 230 tetraploid wheat accessions under greenhouse conditions. A high level of phenotypic variation was observed in response to race TTKSK in the AM panel, allowing for genome-wide association mapping of resistance QTL in wild, landrace, and cultivated tetraploid wheats. Thirty-five resistance QTL were identified on all chromosomes, and seventeen are of particular interest as identified by multiple associations. Many of the identified resistance loci were coincident with previously identified rust resistance genes; however, nine on chromosomes 1AL, 2AL, 4AL, 5BL, and 7BS may be novel. To validate AM results, a biparental population of 146 recombinant inbred lines was also considered, which derived from a cross between the resistant cultivar "Cirillo" and susceptible "Neodur." The stem rust resistance of Cirillo was conferred by a single gene on the distal region of chromosome arm 6AL in an interval map coincident with the resistance gene Sr13, and confirmed one of the resistance loci identified by AM. A search for candidate resistance genes was carried out in the regions where QTL were identified, and many of them corresponded to NBS-LRR genes and protein kinases with LRR domains. The results obtained in the present study are of great interest as a high level of genetic variability for resistance to race TTKSK was described in a germplasm panel comprising most of the tetraploid wheat sub-species.

Keywords: association mapping; linkage mapping; resistance genes; stem rust; tetraploid wheat

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