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Vermaas JV, Petridis L, Qi X, et al. Mechanism of lignin inhibition of enzymatic biomass deconstruction. Biotechnol Biofuels. 2015;8:217doi: 10.1186/s13068-015-0379-8.
Vermaas, J. V., Petridis, L., Qi, X., Schulz, R., Lindner, B., & Smith, J. C. (2015). Mechanism of lignin inhibition of enzymatic biomass deconstruction. Biotechnology for biofuels, 8217. https://doi.org/10.1186/s13068-015-0379-8
Vermaas, Josh V, et al. "Mechanism of lignin inhibition of enzymatic biomass deconstruction." Biotechnology for biofuels vol. 8 (2015): 217. doi: https://doi.org/10.1186/s13068-015-0379-8
Vermaas JV, Petridis L, Qi X, Schulz R, Lindner B, Smith JC. Mechanism of lignin inhibition of enzymatic biomass deconstruction. Biotechnol Biofuels. 2015 Dec 21;8:217. doi: 10.1186/s13068-015-0379-8. eCollection 2015. PMID: 26697106; PMCID: PMC4687093.
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Biotechnol Biofuels. 2015 Dec 21;8:217. doi: 10.1186/s13068-015-0379-8. eCollection 2015.

Mechanism of lignin inhibition of enzymatic biomass deconstruction.

Biotechnology for biofuels

Josh V Vermaas, Loukas Petridis, Xianghong Qi, Roland Schulz, Benjamin Lindner, Jeremy C Smith

Affiliations

  1. UT/ORNL Center for Molecular Biophysics, Oak Ridge National Laboratory, 37831 Oak Ridge, TN USA ; Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, 61801 Urbana, IL USA.
  2. UT/ORNL Center for Molecular Biophysics, Oak Ridge National Laboratory, 37831 Oak Ridge, TN USA.
  3. UT/ORNL Center for Molecular Biophysics, Oak Ridge National Laboratory, 37831 Oak Ridge, TN USA ; Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, 37996 Knoxville, TN USA.
  4. UT/ORNL Center for Molecular Biophysics, Oak Ridge National Laboratory, 37831 Oak Ridge, TN USA ; Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, 37996 Knoxville, TN USA ; University of Tennessee/Oak Ridge National Laboratory Center for Molecular Biophysics, P.O.Box 2008, Oak Ridge, TN 37831-6309 USA.

PMID: 26697106 PMCID: PMC4687093 DOI: 10.1186/s13068-015-0379-8

Abstract

BACKGROUND: The conversion of plant biomass to ethanol via enzymatic cellulose hydrolysis offers a potentially sustainable route to biofuel production. However, the inhibition of enzymatic activity in pretreated biomass by lignin severely limits the efficiency of this process.

RESULTS: By performing atomic-detail molecular dynamics simulation of a biomass model containing cellulose, lignin, and cellulases (TrCel7A), we elucidate detailed lignin inhibition mechanisms. We find that lignin binds preferentially both to the elements of cellulose to which the cellulases also preferentially bind (the hydrophobic faces) and also to the specific residues on the cellulose-binding module of the cellulase that are critical for cellulose binding of TrCel7A (Y466, Y492, and Y493).

CONCLUSIONS: Lignin thus binds exactly where for industrial purposes it is least desired, providing a simple explanation of why hydrolysis yields increase with lignin removal.

Keywords: Biofuel; Cel7A; Cellulose crystallinity; Lignin

References

  1. Biotechnol Biofuels. 2013 Apr 26;6(1):46 - PubMed
  2. J Agric Food Chem. 1999 Aug;47(8):2991-6 - PubMed
  3. J Chromatogr A. 2011 Aug 5;1218(31):5362-8 - PubMed
  4. J Chem Theory Comput. 2012 Sep 11;8(9):3257-3273 - PubMed
  5. J Biotechnol. 2006 Sep 1;125(2):198-209 - PubMed
  6. Biotechnol Bioeng. 2014 Mar;111(3):485-92 - PubMed
  7. Phys Rev E Stat Nonlin Soft Matter Phys. 2011 Jun;83(6 Pt 1):061911 - PubMed
  8. J Chem Theory Comput. 2009 Oct 13;5(10):2798-808 - PubMed
  9. Bioresour Technol. 2010 Jul;101(13):4900-6 - PubMed
  10. Biotechnol Prog. 2009 May-Jun;25(3):807-19 - PubMed
  11. Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3803-8 - PubMed
  12. Bioinformatics. 2013 Apr 1;29(7):845-54 - PubMed
  13. J Mol Biol. 1998 May 1;278(2):439-56 - PubMed
  14. J Comput Chem. 2008 Nov 30;29(15):2543-64 - PubMed
  15. J Biol Chem. 2011 Apr 1;286(13):11195-201 - PubMed
  16. Biotechnol Bioeng. 2009 Jul 1;103(4):715-24 - PubMed
  17. Science. 2007 Feb 9;315(5813):804-7 - PubMed
  18. Proc Natl Acad Sci U S A. 2003 Jan 21;100(2):484-9 - PubMed
  19. J Chem Theory Comput. 2011 Oct 11;7(10):3162-3180 - PubMed
  20. Curr Opin Biotechnol. 2012 Jun;23(3):338-45 - PubMed
  21. J Am Chem Soc. 2004 Jan 28;126(3):698-9 - PubMed
  22. Science. 2011 Sep 2;333(6047):1279-82 - PubMed
  23. J Phys Chem B. 2011 Apr 14;115(14):4118-27 - PubMed
  24. J Am Chem Soc. 2011 Dec 21;133(50):20277-87 - PubMed
  25. J Biol Chem. 1997 Sep 19;272(38):24016-23 - PubMed
  26. Annu Rev Plant Biol. 2003;54:519-46 - PubMed
  27. Proc Natl Acad Sci U S A. 2013 Sep 3;110(36):14646-51 - PubMed
  28. J Biol Chem. 2015 Sep 11;290(37):22818-26 - PubMed
  29. J Chem Phys. 2007 Jan 7;126(1):014101 - PubMed
  30. Bioresour Technol. 2007 Sep;98(13):2503-10 - PubMed
  31. Biochemistry. 1989 Sep 5;28(18):7241-57 - PubMed
  32. Biotechnol Bioeng. 2008 Dec 1;101(5):913-25 - PubMed
  33. FEBS Lett. 1987 Dec 10;225(1-2):163-7 - PubMed
  34. Biotechnol Bioeng. 2011 Aug;108(8):1788-800 - PubMed
  35. Bioresour Technol. 2012 Jan;103(1):201-8 - PubMed
  36. Proteins. 1992 Dec;14(4):475-82 - PubMed
  37. J Biol Chem. 2014 Jul 25;289(30):20960-9 - PubMed
  38. Science. 2014 May 16;344(6185):1246843 - PubMed
  39. Chem Rev. 2015 Feb 11;115(3):1308-448 - PubMed
  40. J Biol Chem. 2012 Jun 8;287(24):20603-12 - PubMed
  41. Curr Opin Struct Biol. 2015 Apr;31:64-74 - PubMed
  42. Phys Chem Chem Phys. 2015 Jan 7;17(1):358-64 - PubMed
  43. Glycobiology. 2004 Aug;14(8):725-37 - PubMed
  44. J Am Chem Soc. 2002 Aug 7;124(31):9074-82 - PubMed
  45. J Mol Graph. 1996 Feb;14(1):33-8, 27-8 - PubMed
  46. Biotechnol Prog. 2007 Sep-Oct;23(5):1130-7 - PubMed
  47. Nat Biotechnol. 2007 Jul;25(7):759-61 - PubMed
  48. J Phys Chem B. 1998 Apr 30;102(18):3586-616 - PubMed
  49. J Comput Chem. 2009 Feb;30(3):457-67 - PubMed
  50. J Mol Biol. 1998 Jan 16;275(2):309-25 - PubMed
  51. Nat Biotechnol. 2011 Aug 07;29(9):835-9 - PubMed
  52. Biotechnol Biofuels. 2013 Jan 28;6(1):15 - PubMed
  53. J Biotechnol. 2004 Jan 8;107(1):65-72 - PubMed
  54. Biomacromolecules. 2013 Oct 14;14(10):3390-8 - PubMed
  55. Eur J Biochem. 1998 Aug 15;256(1):119-27 - PubMed
  56. J Agric Food Chem. 2006 Feb 8;54(3):597-606 - PubMed
  57. Nature. 2012 Aug 16;488(7411):294-303 - PubMed
  58. J Biol Chem. 2011 Sep 16;286(37):32801-9 - PubMed
  59. Bioresour Technol. 2009 Sep;100(17):3948-62 - PubMed
  60. Biotechnol Bioeng. 2011 Mar;108(3):538-48 - PubMed
  61. Enzyme Microb Technol. 2013 Oct 10;53(5):315-21 - PubMed
  62. Proc Natl Acad Sci U S A. 2014 Sep 2;111(35):E3587-95 - PubMed
  63. Biotechnol Bioeng. 2011 Dec;108(12):2823-34 - PubMed
  64. Science. 2012 Nov 23;338(6110):1055-60 - PubMed
  65. J Biol Chem. 1998 Jun 19;273(25):15458-63 - PubMed
  66. J Phys Chem B. 2010 Jan 28;114(3):1447-53 - PubMed
  67. Biotechnol Biofuels. 2014 May 13;7:71 - PubMed
  68. Science. 2010 Aug 13;329(5993):790-2 - PubMed
  69. J Chem Theory Comput. 2009 Aug 20;5(9):2353-2370 - PubMed
  70. Protein Sci. 1995 Jun;4(6):1056-64 - PubMed
  71. Appl Biochem Biotechnol. 2002 Spring;98-100:641-54 - PubMed
  72. J Chem Theory Comput. 2008 Mar;4(3):435-47 - PubMed
  73. Biotechnol Prog. 1999 Oct 1;15(5):804-816 - PubMed

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