Display options
Cite
You J, Wen L, Roufas A, et al. Expression of HGF and c-Met Proteins in Human Keratoconus Corneas. J Ophthalmol. 2015;2015:852986doi: 10.1155/2015/852986.
You, J., Wen, L., Roufas, A., Hodge, C., Sutton, G., & Madigan, M. C. (2015). Expression of HGF and c-Met Proteins in Human Keratoconus Corneas. Journal of ophthalmology, 2015852986. https://doi.org/10.1155/2015/852986
You, Jingjing, et al. "Expression of HGF and c-Met Proteins in Human Keratoconus Corneas." Journal of ophthalmology vol. 2015 (2015): 852986. doi: https://doi.org/10.1155/2015/852986
You J, Wen L, Roufas A, Hodge C, Sutton G, Madigan MC. Expression of HGF and c-Met Proteins in Human Keratoconus Corneas. J Ophthalmol. 2015;2015:852986. doi: 10.1155/2015/852986. Epub 2015 Nov 30. PMID: 26697215; PMCID: PMC4677219.
Copy Download .nbib Format: NLM
Share it on

J Ophthalmol. 2015;2015:852986. doi: 10.1155/2015/852986. Epub 2015 Nov 30.

Expression of HGF and c-Met Proteins in Human Keratoconus Corneas.

Journal of ophthalmology

Jingjing You, Li Wen, Athena Roufas, Chris Hodge, Gerard Sutton, Michele C Madigan

Affiliations

  1. Save Sight Institute, Discipline of Clinical Ophthalmology, The University of Sydney, Sydney, NSW 2000, Australia.
  2. Save Sight Institute, Discipline of Clinical Ophthalmology, The University of Sydney, Sydney, NSW 2000, Australia ; Vision Eye Institute, Chatswood, NSW 2067, Australia.
  3. Save Sight Institute, Discipline of Clinical Ophthalmology, The University of Sydney, Sydney, NSW 2000, Australia ; Vision Eye Institute, Chatswood, NSW 2067, Australia ; Auckland University, Auckland 1010, New Zealand.
  4. Save Sight Institute, Discipline of Clinical Ophthalmology, The University of Sydney, Sydney, NSW 2000, Australia ; School of Optometry & Vision Science, UNSW, Kensington, NSW 2052, Australia.

PMID: 26697215 PMCID: PMC4677219 DOI: 10.1155/2015/852986

Abstract

Keratoconus (KC) is a progressive degenerative inflammatory-related disease of the human cornea leading to decreased visual function. The pathogenesis of KC remains to be understood. Recent genetic studies indicate that gene variants of an inflammation-related molecule, hepatocyte growth factor (HGF), are associated with an increased susceptibility for developing KC. However HGF protein expression in KC has not been explored. In this initial study, we investigated late-stage KC and control corneas for the expression of HGF and its receptor mesenchymal-epithelial transition factor (c-Met/Met). KC buttons (~8 mm diameter) (n = 10) and whole control corneas (n = 6) were fixed in 10% formalin or 2% paraformaldehyde, paraffin embedded and sectioned. Sections were immunolabelled with HGF and c-Met antibodies, visualised using immunofluorescence, and examined with scanning laser confocal microscopy. Semiquantitative grading was used to compare HGF and c-Met immunostaining in KC and control corneas. Overall, KC corneas showed increased HGF and c-Met immunostaining compared to controls. KC corneal epithelium displayed heterogeneous moderate-to-strong immunoreactivity for HGF and c-Met, particularly in the basal epithelium adjacent to the cone area. Taken together with the recent genetic studies, our results further support a possible role for HGF/c-Met in the pathogenesis of KC.

References

  1. Cont Lens Anterior Eye. 2007 Sep;30(4):223-32 - PubMed
  2. Exp Cell Res. 2007 Sep 10;313(15):3319-25 - PubMed
  3. Curr Eye Res. 1991 Jul;10(7):585-92 - PubMed
  4. Invest Ophthalmol Vis Sci. 2003 Mar;44(3):1048-55 - PubMed
  5. Clin Exp Optom. 2008 Jan;91(1):34-55 - PubMed
  6. Pathology. 2012 Apr;44(3):234-9 - PubMed
  7. PLoS One. 2013 Jun 18;8(6):e66770 - PubMed
  8. J Cataract Refract Surg. 2005 Feb;31(2):412-23 - PubMed
  9. Ophthalmology. 2005 Apr;112(4):654-9 - PubMed
  10. Br J Ophthalmol. 2009 Jun;93(6):820-4 - PubMed
  11. J Cell Physiol. 1995 Apr;163(1):61-79 - PubMed
  12. Invest Ophthalmol Vis Sci. 2011 Oct 31;52(11):8514-9 - PubMed
  13. PLoS One. 2014 Jan 08;9(1):e84067 - PubMed
  14. Invest Ophthalmol Vis Sci. 2014 Apr 09;55(4):2180-90 - PubMed
  15. Eur J Hum Genet. 2015 Nov;23 (11):1581-3 - PubMed
  16. Exp Eye Res. 2012 Mar;96(1):132-7 - PubMed
  17. Ther Adv Med Oncol. 2011 Nov;3(1 Suppl):S7-S19 - PubMed
  18. Ophthalmology. 2015 May;122(5):909-17 - PubMed
  19. Surv Ophthalmol. 1998 Jan-Feb;42(4):297-319 - PubMed
  20. Clin Exp Optom. 2014 May;97(3):234-9 - PubMed
  21. Invest Ophthalmol Vis Sci. 1996 Apr;37(5):727-39 - PubMed
  22. Curr Drug Metab. 2011 Jun;12(5):498-506 - PubMed
  23. PLoS One. 2011 Jan 27;6(1):e16437 - PubMed
  24. Invest Ophthalmol Vis Sci. 2015 Sep;56(10):5831-7 - PubMed
  25. J Genet Genomics. 2015 Mar 20;42(3):87-98 - PubMed
  26. Invest Ophthalmol Vis Sci. 1993 Jul;34(8):2544-61 - PubMed
  27. Acta Ophthalmol. 2012 Jun;90(4):e303-9 - PubMed
  28. Eye (Lond). 2015 Jul;29(7):843-59 - PubMed
  29. Curr Eye Res. 1996 Nov;15(11):1124-31 - PubMed
  30. Cornea. 1997 Jul;16(4):465-71 - PubMed
  31. Invest Ophthalmol Vis Sci. 1999 Sep;40(10):2185-90 - PubMed
  32. Clin Exp Ophthalmol. 2009 Mar;37(2):160-76 - PubMed
  33. Exp Eye Res. 1994 Dec;59(6):665-78 - PubMed
  34. Invest Ophthalmol Vis Sci. 2015 Feb 03;56(2):738-50 - PubMed
  35. Clin Exp Ophthalmol. 2010 Mar;38(2):141-53 - PubMed

Publication Types