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Dokic I, Niklas M, Zimmermann F, et al. Correlation of Particle Traversals with Clonogenic Survival Using Cell-Fluorescent Ion Track Hybrid Detector. Front Oncol. 2015;5:275doi: 10.3389/fonc.2015.00275.
Dokic, I., Niklas, M., Zimmermann, F., Mairani, A., Seidel, P., Krunic, D., Jäkel, O., Debus, J., Greilich, S., & Abdollahi, A. (2015). Correlation of Particle Traversals with Clonogenic Survival Using Cell-Fluorescent Ion Track Hybrid Detector. Frontiers in oncology, 5275. https://doi.org/10.3389/fonc.2015.00275
Dokic, Ivana, et al. "Correlation of Particle Traversals with Clonogenic Survival Using Cell-Fluorescent Ion Track Hybrid Detector." Frontiers in oncology vol. 5 (2015): 275. doi: https://doi.org/10.3389/fonc.2015.00275
Dokic I, Niklas M, Zimmermann F, Mairani A, Seidel P, Krunic D, Jäkel O, Debus J, Greilich S, Abdollahi A. Correlation of Particle Traversals with Clonogenic Survival Using Cell-Fluorescent Ion Track Hybrid Detector. Front Oncol. 2015 Dec 07;5:275. doi: 10.3389/fonc.2015.00275. eCollection 2015. PMID: 26697410; PMCID: PMC4671278.
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Front Oncol. 2015 Dec 07;5:275. doi: 10.3389/fonc.2015.00275. eCollection 2015.

Correlation of Particle Traversals with Clonogenic Survival Using Cell-Fluorescent Ion Track Hybrid Detector.

Frontiers in oncology

Ivana Dokic, Martin Niklas, Ferdinand Zimmermann, Andrea Mairani, Philipp Seidel, Damir Krunic, Oliver Jäkel, Jürgen Debus, Steffen Greilich, Amir Abdollahi

Affiliations

  1. German Cancer Consortium, Translational Radiation Oncology, National Center for Tumor Diseases, German Cancer Research Center, Heidelberg University Medical School , Heidelberg , Germany ; Heidelberg Ion Therapy Center , Heidelberg , Germany ; Heidelberg Institute of Radiation Oncology, National Center for Radiation Research in Oncology , Heidelberg , Germany.
  2. Heidelberg Ion Therapy Center , Heidelberg , Germany ; National Center for Oncological Hadrontherapy , Pavia , Italy.
  3. Light Microscopy Facility, German Cancer Research Center , Heidelberg , Germany.
  4. Heidelberg Ion Therapy Center , Heidelberg , Germany ; Heidelberg Institute of Radiation Oncology, National Center for Radiation Research in Oncology , Heidelberg , Germany ; Division of Medical Physics in Radiation Oncology, German Cancer Research Center , Heidelberg , Germany.
  5. Heidelberg Institute of Radiation Oncology, National Center for Radiation Research in Oncology , Heidelberg , Germany ; Division of Medical Physics in Radiation Oncology, German Cancer Research Center , Heidelberg , Germany.

PMID: 26697410 PMCID: PMC4671278 DOI: 10.3389/fonc.2015.00275

Abstract

Development of novel approaches linking the physical characteristics of particles with biological responses are of high relevance for the field of particle therapy. In radiobiology, the clonogenic survival of cells is considered the gold standard assay for the assessment of cellular sensitivity to ionizing radiation. Toward further development of next generation biodosimeters in particle therapy, cell-fluorescent ion track hybrid detector (Cell-FIT-HD) was recently engineered by our group and successfully employed to study physical particle track information in correlation with irradiation-induced DNA damage in cell nuclei. In this work, we investigated the feasibility of Cell-FIT-HD as a tool to study the effects of clinical beams on cellular clonogenic survival. Tumor cells were grown on the fluorescent nuclear track detector as cell culture, mimicking the standard procedures for clonogenic assay. Cell-FIT-HD was used to detect the spatial distribution of particle tracks within colony-initiating cells. The physical data were associated with radiation-induced foci as surrogates for DNA double-strand breaks, the hallmark of radiation-induced cell lethality. Long-term cell fate was monitored to determine the ability of cells to form colonies. We report the first successful detection of particle traversal within colony-initiating cells at subcellular resolution using Cell-FIT-HD.

Keywords: 53BP1; DNA damage foci; carbon ion irradiation; clonogenic survival; fluorescent nuclear track detector

References

  1. DNA Repair (Amst). 2011 Oct 10;10 (10 ):1071-6 - PubMed
  2. Radiat Oncol. 2013 Jun 11;8:141 - PubMed
  3. Int J Radiat Biol. 2015 Feb;91(2):157-65 - PubMed
  4. J Exp Med. 1956 May 1;103(5):653-66 - PubMed
  5. Phys Med Biol. 2013 Sep 21;58(18):N251-66 - PubMed
  6. Neoplasma. 2015 ;62(5):770-6 - PubMed
  7. Nat Protoc. 2006;1(5):2315-9 - PubMed
  8. Nat Rev Cancer. 2005 Mar;5(3):231-7 - PubMed
  9. Int J Radiat Oncol Biol Phys. 2013 Dec 1;87(5):1141-7 - PubMed
  10. Br J Cancer Suppl. 1984;6:81-90 - PubMed
  11. J Biol Chem. 2003 May 30;278(22):19579-82 - PubMed
  12. Nat Rev Clin Oncol. 2013 Jul;10(7):411-24 - PubMed
  13. Nat Genet. 2001 Mar;27(3):247-54 - PubMed
  14. Nature. 2008 Nov 27;456(7221):524-8 - PubMed
  15. J Radiat Res. 2013 May;54(3):494-514 - PubMed
  16. Mol Cancer Ther. 2005 Oct;4(10):1548-50 - PubMed
  17. Methods Mol Biol. 2012;920:613-26 - PubMed

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