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Kuttappan VA, Vicuña EA, Latorre JD, et al. Evaluation of Gastrointestinal Leakage in Multiple Enteric Inflammation Models in Chickens. Front Vet Sci. 2015;2:66doi: 10.3389/fvets.2015.00066.
Kuttappan, V. A., Vicuña, E. A., Latorre, J. D., Wolfenden, A. D., Téllez, G. I., Hargis, B. M., & Bielke, L. R. (2015). Evaluation of Gastrointestinal Leakage in Multiple Enteric Inflammation Models in Chickens. Frontiers in veterinary science, 266. https://doi.org/10.3389/fvets.2015.00066
Kuttappan, Vivek A, et al. "Evaluation of Gastrointestinal Leakage in Multiple Enteric Inflammation Models in Chickens." Frontiers in veterinary science vol. 2 (2015): 66. doi: https://doi.org/10.3389/fvets.2015.00066
Kuttappan VA, Vicuña EA, Latorre JD, Wolfenden AD, Téllez GI, Hargis BM, Bielke LR. Evaluation of Gastrointestinal Leakage in Multiple Enteric Inflammation Models in Chickens. Front Vet Sci. 2015 Dec 14;2:66. doi: 10.3389/fvets.2015.00066. eCollection 2015. PMID: 26697435; PMCID: PMC4677096.
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Front Vet Sci. 2015 Dec 14;2:66. doi: 10.3389/fvets.2015.00066. eCollection 2015.

Evaluation of Gastrointestinal Leakage in Multiple Enteric Inflammation Models in Chickens.

Frontiers in veterinary science

Vivek A Kuttappan, Eduardo A Vicuña, Juan D Latorre, Amanda D Wolfenden, Guillermo I Téllez, Billy M Hargis, Lisa R Bielke

Affiliations

  1. Department of Poultry Science, University of Arkansas , Fayetteville, AR , USA.

PMID: 26697435 PMCID: PMC4677096 DOI: 10.3389/fvets.2015.00066

Abstract

Enteric inflammation models can help researchers' study methods to improve health and performance and evaluate various growth promoters and dietary formulations targeted to improve performance in poultry. Oral administration of fluorescein isothiocyanate-dextran (FITC-d; 3-5 kDa) and its pericellular mucosal epithelial leakage are an established marker to evaluate enteric inflammation in multiple species. The present study evaluated different methods to induce gut inflammation in poultry based on FITC-d leakage. Four independent experiments were completed with different inflammation treatment groups, and serum FITC-d and/or retention of FITC-d in GI tract were determined. In experiment 1 (n = 10 birds/treatment, broilers, processed at 14 days), groups included control (CON), dextran sodium sulfate (DSS; drinking water at 0.75%) and feed restriction (FRS; 24 h before processing). Experiment 2 (n = 14 birds/treatment, leghorns, processed at 7 days) included CON, DSS, FRS, and rye-based diet (RBD). In experiments 3 and 4 (n = 15 birds/treatment, broilers, processed at 7 days), groups were CON, DSS, high fat diet (HFD), FRS, and RBD. In all experiments, FRS and RBD treatments showed significantly higher serum FITC-d levels compared to the respective CON. This indicates that FRS and RBD results in disruption of the intact barrier of the gastrointestinal tract (GIT), resulting in increased gut permeability. DSS and HFD groups showed elevation of serum FITC-d levels although the magnitude of difference from respective CON was inconsistent between experiments. FRS was the only treatment which consistently showed elevated retention of FITC-d in GIT in all experiments. The results from present studies showed that FRS and RBD, based on serum FITC-d levels, can be robust models to induce gut leakage in birds in different age and species/strains.

Keywords: dextran sodium sulfate; enteric inflammation; feed restriction; rye-based diet; serum FITC-d

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