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Sayer C, Szabo Z, Isupov MN, et al. The Structure of a Novel Thermophilic Esterase from the Planctomycetes Species, Thermogutta terrifontis Reveals an Open Active Site Due to a Minimal 'Cap' Domain. Front Microbiol. 2015;6:1294doi: 10.3389/fmicb.2015.01294.
Sayer, C., Szabo, Z., Isupov, M. N., Ingham, C., & Littlechild, J. A. (2015). The Structure of a Novel Thermophilic Esterase from the Planctomycetes Species, Thermogutta terrifontis Reveals an Open Active Site Due to a Minimal 'Cap' Domain. Frontiers in microbiology, 61294. https://doi.org/10.3389/fmicb.2015.01294
Sayer, Christopher, et al. "The Structure of a Novel Thermophilic Esterase from the Planctomycetes Species, Thermogutta terrifontis Reveals an Open Active Site Due to a Minimal 'Cap' Domain." Frontiers in microbiology vol. 6 (2015): 1294. doi: https://doi.org/10.3389/fmicb.2015.01294
Sayer C, Szabo Z, Isupov MN, Ingham C, Littlechild JA. The Structure of a Novel Thermophilic Esterase from the Planctomycetes Species, Thermogutta terrifontis Reveals an Open Active Site Due to a Minimal 'Cap' Domain. Front Microbiol. 2015 Nov 23;6:1294. doi: 10.3389/fmicb.2015.01294. eCollection 2015. PMID: 26635762; PMCID: PMC4655241.
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Front Microbiol. 2015 Nov 23;6:1294. doi: 10.3389/fmicb.2015.01294. eCollection 2015.

The Structure of a Novel Thermophilic Esterase from the Planctomycetes Species, Thermogutta terrifontis Reveals an Open Active Site Due to a Minimal 'Cap' Domain.

Frontiers in microbiology

Christopher Sayer, Zalan Szabo, Michail N Isupov, Colin Ingham, Jennifer A Littlechild

Affiliations

  1. The Henry Wellcome Building for Biocatalysis, Biosciences, College of Life and Environmental Sciences, University of Exeter Exeter, UK.
  2. Microdish BV Utrecht, Netherlands.

PMID: 26635762 PMCID: PMC4655241 DOI: 10.3389/fmicb.2015.01294

Abstract

A carboxyl esterase (TtEst2) has been identified in a novel thermophilic bacterium, Thermogutta terrifontis from the phylum Planctomycetes and has been cloned and over-expressed in Escherichia coli. The enzyme has been characterized biochemically and shown to have activity toward small p-nitrophenyl (pNP) carboxylic esters with optimal activity for pNP-acetate. The enzyme shows moderate thermostability retaining 75% activity after incubation for 30 min at 70°C. The crystal structures have been determined for the native TtEst2 and its complexes with the carboxylic acid products propionate, butyrate, and valerate. TtEst2 differs from most enzymes of the α/β-hydrolase family 3 as it lacks the majority of the 'cap' domain and its active site cavity is exposed to the solvent. The bound ligands have allowed the identification of the carboxyl pocket in the enzyme active site. Comparison of TtEst2 with structurally related enzymes has given insight into how differences in their substrate preference can be rationalized based upon the properties of their active site pockets.

Keywords: Planctomycetes; X-ray structure; biocatalysis; carboxyl esterase; thermophilic enzymes

References

  1. Protein Pept Lett. 2009;16(10):1137-48 - PubMed
  2. Biochim Biophys Acta. 2006 Oct;1764(10):1601-6 - PubMed
  3. Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501 - PubMed
  4. Curr Opin Struct Biol. 1999 Dec;9(6):732-7 - PubMed
  5. Acta Crystallogr D Biol Crystallogr. 2013 Jul;69(Pt 7):1204-14 - PubMed
  6. Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):191-205 - PubMed
  7. Acta Crystallogr D Biol Crystallogr. 2008 Jan;64(Pt 1):33-9 - PubMed
  8. FEBS J. 2015 Aug;282(15):2846-57 - PubMed
  9. Biochem Soc Trans. 2007 Dec;35(Pt 6):1558-63 - PubMed
  10. Structure. 2000 Feb 15;8(2):143-51 - PubMed
  11. Acta Crystallogr D Biol Crystallogr. 2013 Jul;69(Pt 7):1260-73 - PubMed
  12. Proteins. 2008 Jun;71(4):1721-31 - PubMed
  13. Front Cell Dev Biol. 2014 Nov 19;2:70 - PubMed
  14. J Mol Biol. 2001 Nov 30;314(3):507-18 - PubMed
  15. Adv Protein Chem. 1981;34:167-339 - PubMed
  16. Acta Crystallogr D Biol Crystallogr. 2004 Oct;60(Pt 10):1879-82 - PubMed
  17. Curr Protein Pept Sci. 2000 Sep;1(2):209-35 - PubMed
  18. Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):386-94 - PubMed
  19. Nature. 1969 Jan 25;221(5178):337-40 - PubMed
  20. J Biotechnol. 2014 Nov 20;190:11-7 - PubMed
  21. Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):779-84 - PubMed
  22. FEBS J. 2012 Sep;279(17):3071-84 - PubMed
  23. J Mol Biol. 2007 Mar 16;367 (1):212-23 - PubMed
  24. Structure. 1998 Apr 15;6(4):511-9 - PubMed
  25. Protein Sci. 2002 Mar;11(3):467-78 - PubMed
  26. Nat Methods. 2011 Sep 29;8(10):785-6 - PubMed
  27. Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42 - PubMed
  28. Appl Microbiol Biotechnol. 2010 Aug;87(5):1753-64 - PubMed
  29. Acta Crystallogr D Biol Crystallogr. 2013 Apr;69(Pt 4):635-41 - PubMed
  30. Appl Microbiol Biotechnol. 2011 Aug;91(4):1061-72 - PubMed
  31. J Bacteriol. 1999 Aug;181(15):4568-75 - PubMed
  32. J Mol Biol. 1998 Jun 19;279(4):889-900 - PubMed
  33. Structure. 1999 Mar 15;7(3):237-44 - PubMed
  34. Acta Crystallogr D Biol Crystallogr. 2012 Apr;68(Pt 4):431-40 - PubMed
  35. Biochemistry. 2002 Jan 29;41(4):1364-71 - PubMed
  36. Int J Syst Evol Microbiol. 2015 Mar;65(Pt 3):760-5 - PubMed
  37. J Lipid Res. 2015 Aug;56(8):1616-24 - PubMed
  38. J Mol Biol. 2004 Apr 30;338(3):519-32 - PubMed
  39. PLoS One. 2013 Jul 18;8(7):e69125 - PubMed
  40. FEBS J. 2015 Aug;282(15):2879-94 - PubMed
  41. Nucleic Acids Res. 2014 Jan;42(Database issue):D222-30 - PubMed
  42. Nucleic Acids Res. 2014 Jul;42(Web Server issue):W320-4 - PubMed
  43. Nat Struct Biol. 1995 Mar;2(3):218-23 - PubMed
  44. Structure. 1999 Jun 15;7(6):R141-6 - PubMed
  45. J Mol Biol. 1990 Oct 5;215(3):403-10 - PubMed
  46. BMC Struct Biol. 2007 Jul 12;7:47 - PubMed
  47. J Biol Chem. 2004 Feb 20;279(8):6815-23 - PubMed
  48. Biophys J. 1965 Nov;5(6):909-33 - PubMed
  49. J Biol Chem. 1966 Nov 10;241(21):5137-49 - PubMed
  50. J Microbiol. 2008 Feb;46(1):100-7 - PubMed
  51. Biochem Soc Trans. 2004 Apr;32(Pt 2):255-8 - PubMed
  52. Appl Environ Microbiol. 2005 Feb;71(2):817-25 - PubMed
  53. Acta Crystallogr D Biol Crystallogr. 2013 Nov;69(Pt 11):2251-6 - PubMed
  54. Protein Pept Lett. 2012 Feb;19(2):132-43 - PubMed
  55. Protein Eng. 1992 Apr;5(3):197-211 - PubMed
  56. Nucleic Acids Res. 2010 Jul;38(Web Server issue):W545-9 - PubMed
  57. FEMS Microbiol Rev. 2002 Mar;26(1):73-81 - PubMed
  58. Methods Enzymol. 1997;284:85-107 - PubMed
  59. Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):355-67 - PubMed
  60. Biochem Soc Trans. 2011 Jan;39(1):155-8 - PubMed
  61. Appl Microbiol Biotechnol. 1994 Jun;41(4):425-31 - PubMed
  62. Trends Biochem Sci. 1995 May;20(5):178-9 - PubMed
  63. Science. 1991 Aug 23;253(5022):872-9 - PubMed
  64. Appl Environ Microbiol. 1999 Dec;65(12):5500-3 - PubMed
  65. FEBS J. 2013 Dec;280(24):6658-71 - PubMed
  66. J Mol Biol. 2000 Nov 10;303(5):761-71 - PubMed
  67. Nature. 1991 Jun 6;351(6326):491-4 - PubMed
  68. Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32 - PubMed
  69. Mem Inst Oswaldo Cruz. 2012 Jun;107(4):437-49 - PubMed
  70. Acta Crystallogr D Biol Crystallogr. 2014 Oct;70(Pt 10):2652-66 - PubMed
  71. Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):22-5 - PubMed
  72. J Mol Biol. 2004 Sep 10;342(2):551-61 - PubMed
  73. Acta Crystallogr D Biol Crystallogr. 2014 Oct;70(Pt 10):2607-18 - PubMed

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