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Wang YY, Zhou R, Zhou B, et al. Overexpression of heparanase is associated with preeclampsia by inhibiting invasion of trophocytes. Int J Clin Exp Med. 2015;8(10):18107-14
Wang, Y. Y., Zhou, R., Zhou, B., Wang, T., Zhang, L., & Luo, D. (2015). Overexpression of heparanase is associated with preeclampsia by inhibiting invasion of trophocytes. International journal of clinical and experimental medicine, 8(10), 18107-14.
Wang, Yan-Yun, et al. "Overexpression of heparanase is associated with preeclampsia by inhibiting invasion of trophocytes." International journal of clinical and experimental medicine vol. 8,10 (2015): 18107-14.
Wang YY, Zhou R, Zhou B, Wang T, Zhang L, Luo D. Overexpression of heparanase is associated with preeclampsia by inhibiting invasion of trophocytes. Int J Clin Exp Med. 2015 Oct 15;8(10):18107-14. eCollection 2015. PMID: 26770407; PMCID: PMC4694307.
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Int J Clin Exp Med. 2015 Oct 15;8(10):18107-14. eCollection 2015.

Overexpression of heparanase is associated with preeclampsia by inhibiting invasion of trophocytes.

International journal of clinical and experimental medicine

Yan-Yun Wang, Rong Zhou, Bin Zhou, Tao Wang, Lin Zhang, Dong Luo

Affiliations

  1. Laboratory of Molecular Translational Medicine, West China Institute of Women and Children's Health, Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University Chengdu 610041, Sichuan, China.
  2. Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University Chengdu 610041, Sichuan, China.
  3. Department of Anesthesiology, West China Second University Hospital, Sichuan University Chengdu 610041, Sichuan, China.

PMID: 26770407 PMCID: PMC4694307

Abstract

BACKGROUND: Preeclampsia is associated with inadequate invasion of trophocytes and spiral artery remodeling. As a β-D-glucuronidase enzyme, Heparanase is related to tumor angiogenesis, development and invasion. Trophocytes have similar characteristics to tumor cells, and heparanase could therefore play an important role in the pathogenesis of preeclampsia.

METHODS: The expression of heparanase in severe preeclampsia and normal placentas was detected via real-time PCR, immunohistochemistry and western blotting. The effects of heparanase on trophocytes migration and invasion were investigated by culturing the HTR-8/Svneo cell line with recombinant human heparanase protein in vitro.

RESULTS: The levels of inactive 65-kDa heterologous heparanase dimers were obviously increased, and the content of the 50-kDa active polypeptide was decreased in severe preeclampsia. Furthermore, exogenous heparanase protein could reduce the migration and invasion of HTR-8/Svneo cells.

CONCLUSION: Our results suggested that heparanase might be an important factor in the pathogenesis of severe preeclampsia.

Keywords: Preeclampsia; heparanase; invasion; trophocyte

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