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Baek KH, Park SK, Lee MH, et al. Development of Rapid Diagnostic Kit for Identification of Hanwoo (Korean Native Cattle) Brand Meat by Detecting BIO-TAG. Korean J Food Sci Anim Resour. 2014;34(3):339-45doi: 10.5851/kosfa.2014.34.3.339.
Baek, K. H., Park, S. K., Lee, M. H., Kim, S. I., Cho, S. H., & Choi, C. B. (2014). Development of Rapid Diagnostic Kit for Identification of Hanwoo (Korean Native Cattle) Brand Meat by Detecting BIO-TAG. Korean journal for food science of animal resources, 34(3), 339-45. https://doi.org/10.5851/kosfa.2014.34.3.339
Baek, Kyung Hoon, et al. "Development of Rapid Diagnostic Kit for Identification of Hanwoo (Korean Native Cattle) Brand Meat by Detecting BIO-TAG." Korean journal for food science of animal resources vol. 34,3 (2014): 339-45. doi: https://doi.org/10.5851/kosfa.2014.34.3.339
Baek KH, Park SK, Lee MH, Kim SI, Cho SH, Choi CB. Development of Rapid Diagnostic Kit for Identification of Hanwoo (Korean Native Cattle) Brand Meat by Detecting BIO-TAG. Korean J Food Sci Anim Resour. 2014;34(3):339-45. doi: 10.5851/kosfa.2014.34.3.339. Epub 2014 Jun 30. PMID: 26761175; PMCID: PMC4597872.
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Korean J Food Sci Anim Resour. 2014;34(3):339-45. doi: 10.5851/kosfa.2014.34.3.339. Epub 2014 Jun 30.

Development of Rapid Diagnostic Kit for Identification of Hanwoo (Korean Native Cattle) Brand Meat by Detecting BIO-TAG.

Korean journal for food science of animal resources

Kyung Hoon Baek, Sung Kwon Park, Myung Hoon Lee, Sung Il Kim, Soo Hyun Cho, Chang Bon Choi

Affiliations

  1. School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Korea.
  2. National Institute of Animal Resource, RDA, Suwon 441-706, Korea.
  3. D&P Biotech, Daegu 700-422, Korea.

PMID: 26761175 PMCID: PMC4597872 DOI: 10.5851/kosfa.2014.34.3.339

Abstract

This study was performed to develop a rapid immuno-assay kit, by using a specific antigen to detect Hanwoo brand meat. We selected a synthetic antigen specific to our target antibody, named BIO-TAG (Tyr-D-Ala-Phe), by utilizing a computer-based analysis and literature review. BIO-TAG tagged with adjuvant was subcutaneously injected in sheep and Hanwoo. The serum and meat juice of the immunized or non-immunized animal were then analyzed, to measure the titer of antibody by ELISA and Western blot. The amount of antibodies against the BIO-TAG increased (p<0.05) in serum by vaccination. Furthermore, meat juice from the immunized Hanwoo showed greater (p<0.05) antibody titer, compared with those from non-immunized groups. To optimze the dilution factor, we performed dot-ELISA, with various combination levels of BIO-TAG. Results from dot-ELISA showed that 2 mg/mL BIO-TAG was sufficient to distinguish the immunized meat from non-immunized groups. These results support our hypothesis that simple immunization of Hanwoo generates a sufficient amount of antibodies to be detectable in the meat juice by means of the immune-assay. Therefore, specific Hanwoo brand meat can be more precisely identified by our rapid diagnostic kit. This technology can deter possible fraud of counterfeit meat brands in the Korean domestic market with ease and rapidity; and offers a new tool that guarantees consumers high quality Hanwoo brand beef.

Keywords: BIO-TAG; Hanwoo; rapid diagnosis kit; synthetic peptide

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