Display options
Cite
Poorabbas B, Mardaneh J, Rezaei Z, et al. Nosocomial Infections: Multicenter surveillance of antimicrobial resistance profile of Staphylococcus aureus and Gram negative rods isolated from blood and other sterile body fluids in Iran. Iran J Microbiol. 2015;7(3):127-35
Poorabbas, B., Mardaneh, J., Rezaei, Z., Kalani, M., Pouladfar, G., Alami, M. H., Soltani, J., Shamsi-Zadeh, A., Abdoli-Oskooi, S., Saffar, M. J., & Alborzi, A. (2015). Nosocomial Infections: Multicenter surveillance of antimicrobial resistance profile of Staphylococcus aureus and Gram negative rods isolated from blood and other sterile body fluids in Iran. Iranian journal of microbiology, 7(3), 127-35.
Poorabbas, Bahman, et al. "Nosocomial Infections: Multicenter surveillance of antimicrobial resistance profile of Staphylococcus aureus and Gram negative rods isolated from blood and other sterile body fluids in Iran." Iranian journal of microbiology vol. 7,3 (2015): 127-35.
Poorabbas B, Mardaneh J, Rezaei Z, Kalani M, Pouladfar G, Alami MH, Soltani J, Shamsi-Zadeh A, Abdoli-Oskooi S, Saffar MJ, Alborzi A. Nosocomial Infections: Multicenter surveillance of antimicrobial resistance profile of Staphylococcus aureus and Gram negative rods isolated from blood and other sterile body fluids in Iran. Iran J Microbiol. 2015 Jun;7(3):127-35. PMID: 26668699; PMCID: PMC4676981.
Copy Download .nbib Format: NLM
Share it on

Iran J Microbiol. 2015 Jun;7(3):127-35.

Nosocomial Infections: Multicenter surveillance of antimicrobial resistance profile of Staphylococcus aureus and Gram negative rods isolated from blood and other sterile body fluids in Iran.

Iranian journal of microbiology

Bahman Poorabbas, Jalal Mardaneh, Zahra Rezaei, Mehdi Kalani, Gholamreza Pouladfar, Mohammad Hasan Alami, Jafar Soltani, Ahmad Shamsi-Zadeh, Shahram Abdoli-Oskooi, Mohammed Jafar Saffar, Abdolvahab Alborzi

Affiliations

  1. Professor Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, IR Iran.
  2. Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
  3. Department of Pediatrics, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IR Iran.
  4. Infectious Diseases and Tropical Medicine Research Center, Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
  5. Pediatric Health Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran.
  6. Mazandaran University of Medical Sciences, Sari, IR Iran.

PMID: 26668699 PMCID: PMC4676981

Abstract

BACKGROUND AND OBJECTIVE: Antibiotic resistance is increasing, especially in healthcare-associated infections causing significant public health concerns worldwide. National information is required to make appropriate policies, update list of essential drugs for treatment, and evaluate the effects of intervention strategies. A nationwide surveillance of antimicrobial resistant bacteria in nosocomial infections was established in Iran in 2008, so that the data obtained through the surveillance would enable us to construct a database.

MATERIALS AND METHODS: Seven major teaching hospitals in Shiraz, Tabriz, Sari, Mashhad, Sanandaj, Ahwaz and Isfahan participated in this study. A total of 858 strains isolated from blood and other sterile body fluids were tested. Identification at the species level was performed with conventional biochemical methods and the API system. Susceptibility tests were done using disk diffusion method. The methicillin-resistance in S. aureus (MRSA) was determined by the oxacillin agar screen plate and respective MIC values were assessed using the E-test strips. The confirmatory disk diffusion methods were applied for phenotypic identification of extended-spectrum β- lactamase (ESBL) production for E. coli and K. pneumoniae, according to CLSI guidelines.

RESULTS: Cultivation and re-identification of the strains yielded 858 isolates, consisting of 224 S. aureus, 148 Klebsiella spp., 105 Serratia spp., 146 E. coli, 67 Acinetobacter spp., 38 Enterobacter spp., 95 Pseudomonas spp., 71 P.aeruginosa. 35 Stenotrophomonas sp., and 8 other organisms. MRSA was detected in 37.5% of the isolates. No vancomycin-resistant or vancomycin-intermediate resistant S. aureus was detected. With the exception of Acinetobacter and Stenotrophomonas, 85% of the Gram-negative isolates were found to be susceptible in vitro to imipenem. Overall, about 61% of K. pneumoniae and 35% of E. coli isolates were ESBL producing.

CONCLUSION: Multidrug resistant isolates of Gram-negative organisms and methicillin-resistant strains of S. aureus have been detected in many hospitals in this study.

Keywords: Antimicrobial resistance; Blood; Gram negative bacteria; Iran; Staphylococcus aureus; Sterile body fluids

References

  1. J Infect Dis. 1994 Dec;170(6):1622-5 - PubMed
  2. Clin Microbiol Infect. 1999 Jul;5(7):355-363 - PubMed
  3. Antimicrob Resist Infect Control. 2013 Jun 12;2:20 - PubMed
  4. Emerg Infect Dis. 2011 Jun;17(6):1064-7 - PubMed
  5. Lancet Infect Dis. 2013 Dec;13(12):1057-98 - PubMed
  6. Clin Microbiol Rev. 2012 Jan;25(1):2-41 - PubMed
  7. Am J Med. 2006 Jun;119(6 Suppl 1):S11-9; discussion S62-70 - PubMed
  8. Am J Respir Crit Care Med. 2000 Nov;162(5):1610-6 - PubMed
  9. PLoS One. 2013 Jul 23;8(7):e69581 - PubMed
  10. Pathology. 1996 Nov;28(4):359-63 - PubMed
  11. Trans R Soc Trop Med Hyg. 2014 Jan;108(1):22-8 - PubMed
  12. Clin Infect Dis. 2011 Feb 1;52(3):285-92 - PubMed
  13. Arch Trauma Res. 2014 Jun 01;3(2):e18182 - PubMed
  14. J Infect Dev Ctries. 2012 Feb 13;6(2):120-5 - PubMed
  15. Braz J Infect Dis. 2012 Jul-Aug;16(4):351-6 - PubMed
  16. Jundishapur J Microbiol. 2014 Jun;7(6):e10608 - PubMed
  17. Cell Mol Life Sci. 2004 Sep;61(17):2200-23 - PubMed
  18. Iran J Microbiol. 2013 Sep;5(3):263-7 - PubMed
  19. Int J Antimicrob Agents. 2012 Feb;39(2):105-14 - PubMed
  20. Antimicrob Agents Chemother. 2009 Aug;53(8):3280-4 - PubMed
  21. Int J Infect Dis. 2007 Nov;11(6):544-8 - PubMed
  22. Clin Infect Dis. 1997 Feb;24(2):211-5 - PubMed
  23. Infect Control Hosp Epidemiol. 2013 Mar;34(3):259-68 - PubMed
  24. Iran J Pediatr. 2014 Jun;24(3):261-6 - PubMed
  25. Clin Microbiol Infect. 2012 Mar;18(3):268-81 - PubMed
  26. J Microbiol Immunol Infect. 2005 Dec;38(6):376-82 - PubMed
  27. Biomed Res Int. 2013;2013:860467 - PubMed
  28. Antimicrob Agents Chemother. 2007 Oct;51(10):3471-84 - PubMed
  29. Clin Infect Dis. 2008 Jun 1;46 Suppl 5:S344-9 - PubMed
  30. Antimicrob Agents Chemother. 2006 Aug;50(8):2872-4 - PubMed
  31. J Clin Microbiol. 2005 Sep;43(9):4916-7 - PubMed
  32. Clin Infect Dis. 2013 Sep;57(6):781-8 - PubMed
  33. PLoS One. 2013 Sep 12;8(9):e73300 - PubMed

Publication Types