Display options
Cite
Eftekhar F, Naseh Z. Extended-spectrum β-lactamase and carbapenemase production among burn and non-burn clinical isolates of Klebsiella pneumoniae. Iran J Microbiol. 2015;7(3):144-9
Eftekhar, F., & Naseh, Z. (2015). Extended-spectrum β-lactamase and carbapenemase production among burn and non-burn clinical isolates of Klebsiella pneumoniae. Iranian journal of microbiology, 7(3), 144-9.
Eftekhar, Fereshteh, and Naseh, Ziaeldin. "Extended-spectrum β-lactamase and carbapenemase production among burn and non-burn clinical isolates of Klebsiella pneumoniae." Iranian journal of microbiology vol. 7,3 (2015): 144-9.
Eftekhar F, Naseh Z. Extended-spectrum β-lactamase and carbapenemase production among burn and non-burn clinical isolates of Klebsiella pneumoniae. Iran J Microbiol. 2015 Jun;7(3):144-9. PMID: 26668701; PMCID: PMC4676983.
Copy Download .nbib Format: NLM
Share it on

Iran J Microbiol. 2015 Jun;7(3):144-9.

Extended-spectrum β-lactamase and carbapenemase production among burn and non-burn clinical isolates of Klebsiella pneumoniae.

Iranian journal of microbiology

Fereshteh Eftekhar, Ziaeldin Naseh

Affiliations

  1. Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran.

PMID: 26668701 PMCID: PMC4676983

Abstract

BACKGROUND AND OBJECTIVES: Klebsiella pneumoniae is an opportunistic pathogen responsible for up to 10% of nosocomial infections. The emergence and spread of multidrug resistant K. pneumoniae, mostly due to the production of extended-spectrum β-lactamases (ESBL) and carbapenemases, is often responsible for antibiotic treatment failure of these infections. We compared the antibiotic resistance profiles, ESBL and carbapenemase production as well as presence of KPC-type genes in burn and non-burn clinical isolates of K. pneumoniae.

MATERIALS AND METHODS: Fifty five clinical isolates were collected from Shahid Motahari (25 burn isolates) and Shariati (30 non-burn isolates) hospitals between August 2011 to January 2012. Antibiotic susceptibility was determined to 12 antibiotics using disc diffusion. The phenotypic confirmatory test (PCT) was used to screen for ESBL production. Carbapenemase activity was measured by the modified Hodge test (MHT) and KPC-type carbapenemases were further sought by PCR using specific primers.

RESULTS: Both groups were highly resistant to cefotaxime and ceftazidime (>92%). Burn isolates were significantly more resistant to cefepime, amoxiclav, imipenem, meropenem, gentamicin and ciprofloxacin compared to the non-burn strains (p<0.05). No significant differences were observed in ESBL production between the two groups. Carbapenem resistance was only observed among the burn isolates (n=5, 9.1%). Five carbapenem-resistant isolates produced carbapenemases. However, none of the isolates harbored the KPC-type genes.

CONCLUSION: Higher rates of drug resistance were observed in burn isolates of K. pneumoniae compared to the non-burn strains. Carbapenemase phenotype was only observed among the burn isolates but KPC-type gene was not detected.

Keywords: ESBL; KPC; Klebsiella pneumoniae; carbapenemase; extended-spectrum β-lactamase

References

  1. J Clin Microbiol. 2007 Aug;45(8):2723-5 - PubMed
  2. Int J Antimicrob Agents. 2010 Nov;36 Suppl 3:S8-14 - PubMed
  3. Ann Burns Fire Disasters. 2012 Jun 30;25(2):74-7 - PubMed
  4. Clin Microbiol Rev. 2005 Oct;18(4):657-86 - PubMed
  5. Clin Microbiol Rev. 2012 Oct;25(4):682-707 - PubMed
  6. Antimicrob Agents Chemother. 2006 Aug;50(8):2880-2 - PubMed
  7. J Infect Dev Ctries. 2010 Oct 28;4(10):609-15 - PubMed
  8. Sao Paulo Med J. 2012;130(1):37-43 - PubMed
  9. Clin Microbiol Rev. 2007 Jul;20(3):440-58, table of contents - PubMed
  10. J Clin Microbiol. 2005 Jun;43(6):2551-8 - PubMed
  11. Iran J Microbiol. 2015 Apr;7(2):123-4 - PubMed
  12. Biotechnol Lett. 2006 Jan;28(1):55-9 - PubMed
  13. Clin Microbiol Rev. 1998 Oct;11(4):589-603 - PubMed
  14. J Infect Dev Ctries. 2010 Mar 29;4(3):132-8 - PubMed
  15. Antimicrob Agents Chemother. 2001 Apr;45(4):1151-61 - PubMed
  16. Int J Antimicrob Agents. 2010 Nov;36(5):430-5 - PubMed
  17. Clin Microbiol Rev. 2001 Oct;14(4):933-51, table of contents - PubMed
  18. Clin Infect Dis. 2004 Jul 1;39(1):55-60 - PubMed
  19. Clin Infect Dis. 2009 Dec 1;49(11):1739-41 - PubMed
  20. Antimicrob Agents Chemother. 2011 Nov;55(11):4943-60 - PubMed
  21. Antimicrob Agents Chemother. 2005 Oct;49(10):4423-4 - PubMed
  22. Euro Surveill. 2008 Nov 20;13(47):null - PubMed

Publication Types