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Bohari SP, Grover LM, Hukins DW. Pulsed low-intensity ultrasound increases proliferation and extracelluar matrix production by human dermal fibroblasts in three-dimensional culture. J Tissue Eng. 2015;6:2041731415615777doi: 10.1177/2041731415615777.
Bohari, S. P., Grover, L. M., & Hukins, D. W. (2015). Pulsed low-intensity ultrasound increases proliferation and extracelluar matrix production by human dermal fibroblasts in three-dimensional culture. Journal of tissue engineering, 62041731415615777. https://doi.org/10.1177/2041731415615777
Bohari, Siti Pm, et al. "Pulsed low-intensity ultrasound increases proliferation and extracelluar matrix production by human dermal fibroblasts in three-dimensional culture." Journal of tissue engineering vol. 6 (2015): 2041731415615777. doi: https://doi.org/10.1177/2041731415615777
Bohari SP, Grover LM, Hukins DW. Pulsed low-intensity ultrasound increases proliferation and extracelluar matrix production by human dermal fibroblasts in three-dimensional culture. J Tissue Eng. 2015 Nov 19;6:2041731415615777. doi: 10.1177/2041731415615777. eCollection 2015. PMID: 26668710; PMCID: PMC4674020.
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J Tissue Eng. 2015 Nov 19;6:2041731415615777. doi: 10.1177/2041731415615777. eCollection 2015.

Pulsed low-intensity ultrasound increases proliferation and extracelluar matrix production by human dermal fibroblasts in three-dimensional culture.

Journal of tissue engineering

Siti Pm Bohari, Liam M Grover, David Wl Hukins

Affiliations

  1. Faculty of Biosciences and Biomedical Engineering, Universiti Teknologi Malaysia, Johor Bahru, Malaysia ; School of Chemical Engineering, University of Birmingham, Birmingham, UK ; School of Mechanical Engineering, University of Birmingham, Birmingham, UK.
  2. School of Chemical Engineering, University of Birmingham, Birmingham, UK.
  3. School of Mechanical Engineering, University of Birmingham, Birmingham, UK.

PMID: 26668710 PMCID: PMC4674020 DOI: 10.1177/2041731415615777

Abstract

This study evaluated the effect of pulsed low-intensity ultrasound on cell proliferation, collagen production and glycosaminoglycan deposition by human dermal fibroblasts encapsulated in alginate. Hoechst 33258 assay for cell number, hydroxyproline assay for collagen content, dimethylmethylene blue assay for glycosaminoglycan content and scanning electron microscopy were performed on the encapsulated cells treated with pulsed low-intensity ultrasound and a control group that remained untreated. Pulsed low-intensity ultrasound showed a significant effect on cell proliferation and collagen deposition but no consistent pattern for glycosaminoglycan content. Alcian blue staining showed that glycosaminoglycans were deposited around the cells in both treated and control groups. These results suggest that pulsed low-intensity ultrasound alone shows a positive effect on cell proliferation and collagen deposition even without growth factor supplements.

Keywords: Alginate; collagen; glycosaminoglycan; human dermal fibroblast; proliferation; ultrasound

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