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Goto M, Hayata K, Chiba J, et al. Multiplex cytokine analysis of Werner syndrome. Intractable Rare Dis Res. 2015;4(4):190-7doi: 10.5582/irdr.2015.01035.
Goto, M., Hayata, K., Chiba, J., Matsuura, M., Iwaki-Egawa, S., & Watanabe, Y. (2015). Multiplex cytokine analysis of Werner syndrome. Intractable & rare diseases research, 4(4), 190-7. https://doi.org/10.5582/irdr.2015.01035
Goto, Makoto, et al. "Multiplex cytokine analysis of Werner syndrome." Intractable & rare diseases research vol. 4,4 (2015): 190-7. doi: https://doi.org/10.5582/irdr.2015.01035
Goto M, Hayata K, Chiba J, Matsuura M, Iwaki-Egawa S, Watanabe Y. Multiplex cytokine analysis of Werner syndrome. Intractable Rare Dis Res. 2015 Nov;4(4):190-7. doi: 10.5582/irdr.2015.01035. PMID: 26668779; PMCID: PMC4660860.
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Intractable Rare Dis Res. 2015 Nov;4(4):190-7. doi: 10.5582/irdr.2015.01035.

Multiplex cytokine analysis of Werner syndrome.

Intractable & rare diseases research

Makoto Goto, Koichiro Hayata, Junji Chiba, Masaaki Matsuura, Sachiko Iwaki-Egawa, Yasuhiro Watanabe

Affiliations

  1. Division of Anti-ageing and Longevity Sciences, Department of Medical Technology, Faculty of Medical Engineering, Toin University of Yokohama, Yokohama, Japan ; Department of Orthopaedics & Rheumatology, East Medical Center, Tokyo Women's Medical University, Tokyo, Japan ; Division of Rheumatology, Nerima-Hikarigaoka Hospital, Tokyo, Japan.
  2. Department of Orthopaedics & Rheumatology, East Medical Center, Tokyo Women's Medical University, Tokyo, Japan.
  3. Department of Cancer Genomics, Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan ; Graduate School of Public Health, Teikyo University, Tokyo, Japan.
  4. Department of Life Sciences, School of Pharmacy, Hokkaido Pharmaceutical University, Hokkaido, Japan.

PMID: 26668779 PMCID: PMC4660860 DOI: 10.5582/irdr.2015.01035

Abstract

We reported a minor inflammation-driven ageing (inflammageing) assessed by highly sensitive CRP (hsCRP) in normal individuals and patients with Werner syndrome (WS), followed by an ageing associated Th2-biased cytokine change in normal ageing in the previous papers. To further study the association of hsCRP and 26 cytokines/chemokines in 35 WS patients, a multiple cytokine array system was used in the same serum samples as were examined for hsCRP. The serum levels of Th2 cytokines (IL-4, IL-6, IL-10, and GM-CSF), Th1 products (IL-2, TNFα, IL-12, and IFNγ) and monocyte/macrophage products (MCP-1, basic FGF and G-CSF) in WS were significantly elevated compared with normal ageing. Elevated hsCRP level in WS was significantly correlated with IL-6, IL-12 and VEGF levels, if age and sex were taken into account. A pro-inflammatory cytokine/chemokine circuit-stimulated immunological shift to Th2 in WS was similar to normal ageing. These cytokine/chemokine changes may induce a systemic chronic inflammation monitored by hsCRP, though these immunological changes in WS were more complicated than normal ageing, possibly due to the WS-specific chronic inflammation such as skin ulcer, diabetes mellitus and central obesity with visceral fat deposition. Further study may warrant the pathophysiology of Th2 shift and Th2-biased inflammageing in normal ageing and WS.

Keywords: Ageing; CRP; Werner syndrome; chemokine; cytokine; inflammageing

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