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Han J, Xu Y, Yang D, et al. Effect of Polysaccharides from Acanthopanax senticosus on Intestinal Mucosal Barrier of Escherichia coli Lipopolysaccharide Challenged Mice. Asian-Australas J Anim Sci. 2016;29(1):134-41doi: 10.5713/ajas.15.0534.
Han, J., Xu, Y., Yang, D., Yu, N., Bai, Z., & Bian, L. (2016). Effect of Polysaccharides from Acanthopanax senticosus on Intestinal Mucosal Barrier of Escherichia coli Lipopolysaccharide Challenged Mice. Asian-Australasian journal of animal sciences, 29(1), 134-41. https://doi.org/10.5713/ajas.15.0534
Han, Jie, et al. "Effect of Polysaccharides from Acanthopanax senticosus on Intestinal Mucosal Barrier of Escherichia coli Lipopolysaccharide Challenged Mice." Asian-Australasian journal of animal sciences vol. 29,1 (2016): 134-41. doi: https://doi.org/10.5713/ajas.15.0534
Han J, Xu Y, Yang D, Yu N, Bai Z, Bian L. Effect of Polysaccharides from Acanthopanax senticosus on Intestinal Mucosal Barrier of Escherichia coli Lipopolysaccharide Challenged Mice. Asian-Australas J Anim Sci. 2016 Jan;29(1):134-41. doi: 10.5713/ajas.15.0534. PMID: 26732337; PMCID: PMC4698680.
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Asian-Australas J Anim Sci. 2016 Jan;29(1):134-41. doi: 10.5713/ajas.15.0534.

Effect of Polysaccharides from Acanthopanax senticosus on Intestinal Mucosal Barrier of Escherichia coli Lipopolysaccharide Challenged Mice.

Asian-Australasian journal of animal sciences

Jie Han, Yunhe Xu, Di Yang, Ning Yu, Zishan Bai, Lianquan Bian

Affiliations

  1. College of Animal Husbandry and Veterinary, Liaoning Medical University, Jinzhou 121-001, China .
  2. Institute of Biotechnology Research, Liaoning Academy of Agricultural Sciences, Shenyang 1108-66, China .

PMID: 26732337 PMCID: PMC4698680 DOI: 10.5713/ajas.15.0534

Abstract

To investigate the role of polysaccharide from Acanthopanax senticosus (ASPS) in preventing lipopolysaccharide (LPS)-induced intestinal injury, 18 mice (at 5 wk of age) were assigned to three groups with 6 replicates of one mouse each. Mice were administrated by oral gavage with or without ASPS (300 mg/kg body weight) for 14 days and were injected with saline or LPS at 15 days. Intestinal samples were collected at 4 h post-challenge. The results showed that ASPS ameliorated LPS-induced deterioration of digestive ability of LPS-challenged mice, indicated by an increase in intestinal lactase activity (45%, p<0.05), and the intestinal morphology, as proved by improved villus height (20.84%, p<0.05) and villus height:crypt depth ratio (42%, p<0.05), and lower crypt depth in jejunum (15.55%, p<0.05), as well as enhanced intestinal tight junction proteins expression involving occludin-1 (71.43%, p<0.05). ASPS also prevented intestinal inflammation response, supported by decrease in intestinal inflammatory mediators including tumor necrosis factor α (22.28%, p<0.05) and heat shock protein (HSP70) (77.42%, p<0.05). In addition, intestinal mucus layers were also improved by ASPS, as indicated by the increase in number of goblet cells (24.89%, p<0.05) and intestinal trefoil peptide (17.75%, p<0.05). Finally, ASPS facilitated mRNA expression of epidermal growth factor (100%, p<0.05) and its receptor (200%, p<0.05) gene. These results indicate that ASPS can prevent intestinal mucosal barrier injury under inflammatory conditions, which may be associated with up-regulating gene mRNA expression of epidermal growth factor and its receptor.

Keywords: Epidermal Growth Factor; Herbal Extract; Inflammation; Intestinal Barrier; Metabolism; Pro-inflammatory Cytokines

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