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Azuma K, Hirao Y, Hayakawa Y, et al. Application of Pre-Column Labeling Liquid Chromatography for Canine Plasma-Free Amino Acid Analysis. Metabolites. 2016;6(1)doi: 10.3390/metabo6010003.
Azuma, K., Hirao, Y., Hayakawa, Y., Murahata, Y., Osaki, T., Tsuka, T., Imagawa, T., Okamoto, Y., & Ito, N. (2016). Application of Pre-Column Labeling Liquid Chromatography for Canine Plasma-Free Amino Acid Analysis. Metabolites, 6(1), . https://doi.org/10.3390/metabo6010003
Azuma, Kazuo, et al. "Application of Pre-Column Labeling Liquid Chromatography for Canine Plasma-Free Amino Acid Analysis." Metabolites vol. 6,1 (2016). doi: https://doi.org/10.3390/metabo6010003
Azuma K, Hirao Y, Hayakawa Y, Murahata Y, Osaki T, Tsuka T, Imagawa T, Okamoto Y, Ito N. Application of Pre-Column Labeling Liquid Chromatography for Canine Plasma-Free Amino Acid Analysis. Metabolites. 2016 Jan 12;6(1). doi: 10.3390/metabo6010003. PMID: 26771650; PMCID: PMC4812332.
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Metabolites. 2016 Jan 12;6(1). doi: 10.3390/metabo6010003.

Application of Pre-Column Labeling Liquid Chromatography for Canine Plasma-Free Amino Acid Analysis.

Metabolites

Kazuo Azuma, Yoshiko Hirao, Yoshihiro Hayakawa, Yusuke Murahata, Tomohiro Osaki, Takeshi Tsuka, Tomohiro Imagawa, Yoshiharu Okamoto, Norihiko Ito

Affiliations

  1. Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-minami, Tottori 680-8533, Japan. [email protected].
  2. Analytical and Measuring Instruments Division, Shimadzu Corporation, 1, Nishinokyo Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan. [email protected].
  3. Analytical and Measuring Instruments Division, Shimadzu Corporation, 1, Nishinokyo Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan. [email protected].
  4. Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-minami, Tottori 680-8533, Japan. [email protected].
  5. Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-minami, Tottori 680-8533, Japan. [email protected].
  6. Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-minami, Tottori 680-8533, Japan. [email protected].
  7. Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-minami, Tottori 680-8533, Japan. [email protected].
  8. Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-minami, Tottori 680-8533, Japan. [email protected].
  9. Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-minami, Tottori 680-8533, Japan. [email protected].

PMID: 26771650 PMCID: PMC4812332 DOI: 10.3390/metabo6010003

Abstract

Plasma-free amino acid (PFAA) levels are a useful metric for diagnosing cancer and providing a prognosis. However, the use of analysis of PFAA levels has been limited in the veterinary medicine field. We addressed the application of liquid chromatography (LC) using a pre-column labeling technique for analysis of canine PFAA levels. This method significantly shortened the analysis time relative to conventional methods. No diurnal fluctuations were detected at 9:00 AM in most PFAA levels, and food intake increased the levels of some PFAAs, including valine, leucine, tyrosine, phenylalanine, and proline. These results indicate that LC with pre-column labeling is useful for measuring canine PFAA levels, for which time of day and interval after food intake must be taken into consideration.

Keywords: Plasma-free amino acid; canine plasma amino acid levels; liquid chromatography measurements; pre- and post-food intake; pre-column labeling

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