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Sharma C, Badyal PN, Rawal RK. Simultaneous Estimation of Hydrochlorothiazide, Hydralazine Hydrochloride, and Reserpine Using PCA, NAS, and NAS-PCA. Sci Pharm. 2015;83(4):599-610doi: 10.3797/scipharm.1505-03.
Sharma, C., Badyal, P. N., & Rawal, R. K. (2015). Simultaneous Estimation of Hydrochlorothiazide, Hydralazine Hydrochloride, and Reserpine Using PCA, NAS, and NAS-PCA. Scientia pharmaceutica, 83(4), 599-610. https://doi.org/10.3797/scipharm.1505-03
Sharma, Chetan, et al. "Simultaneous Estimation of Hydrochlorothiazide, Hydralazine Hydrochloride, and Reserpine Using PCA, NAS, and NAS-PCA." Scientia pharmaceutica vol. 83,4 (2015): 599-610. doi: https://doi.org/10.3797/scipharm.1505-03
Sharma C, Badyal PN, Rawal RK. Simultaneous Estimation of Hydrochlorothiazide, Hydralazine Hydrochloride, and Reserpine Using PCA, NAS, and NAS-PCA. Sci Pharm. 2015 Jun 22;83(4):599-610. doi: 10.3797/scipharm.1505-03. Print 2015. PMID: 26839841; PMCID: PMC4727726.
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Sci Pharm. 2015 Jun 22;83(4):599-610. doi: 10.3797/scipharm.1505-03. Print 2015.

Simultaneous Estimation of Hydrochlorothiazide, Hydralazine Hydrochloride, and Reserpine Using PCA, NAS, and NAS-PCA.

Scientia pharmaceutica

Chetan Sharma, Pragya Nand Badyal, Ravindra K Rawal

Affiliations

  1. Department of Pharmaceutical Analysis, Indo-Soviet Friendship College of Pharmacy (ISFCP), Moga 142001, India.

PMID: 26839841 PMCID: PMC4727726 DOI: 10.3797/scipharm.1505-03

Abstract

In this study, new and feasible UV-visible spectrophotometric and multivariate spectrophotometric methods were described for the simultaneous determination of hydrochlorothiazide (HCTZ), hydralazine hydrochloride (H.HCl), and reserpine (RES) in combined pharmaceutical tablets. Methanol was used as a solvent for analysis and the whole UV region was scanned from 200-400 nm. The resolution was obtained by using multivariate methods such as the net analyte signal method (NAS), principal component analysis (PCA), and net analyte signal-principal component analysis (NAS-PCA) applied to the UV spectra of the mixture. The results obtained from all of the three methods were compared. NAS-PCA showed a lot of resolved data as compared to NAS and PCA. Thus, the NAS-PCA technique is a combination of NAS and PCA methods which is advantageous to obtain the information from overlapping results.

Keywords: Net Analyte Signal; Principal Component Analysis; Relative Standard Error of Prediction

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