Anal Chem. 2016 Mar 01;88(5):2856-63. doi: 10.1021/acs.analchem.5b04583. Epub 2016 Feb 16.

HPLC-MS with Glass Chips Featuring Monolithically Integrated Electrospray Emitters of Different Geometries.

Analytical chemistry

Carsten Lotter, Josef J Heiland, Sebastian Thurmann, Laura Mauritz, Detlev Belder

PMID: 26840982 DOI: 10.1021/acs.analchem.5b04583

Abstract

We present and evaluate an approach for coupling liquid chromatography in glass chips with mass spectrometry via fully integrated electrospray emitters. We developed an instrumental platform which allows a robust and reproducible operation of high performance chip chromatography coupled to mass spectrometry. A comparison of differently shaped emitters, from flat over edged to pulled geometries, revealed that all types performed equally well for typical nano-HPLC flow rates. At very low flow rates below 50 nL·min(-1) very sharp, pulled nanospray emitters turned out to be mandatory for the generation of a stable electrospray.

Publication Types

• Journal Article
• Research Support, Non-U.S. Gov't