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Han JH, Lee JH, Lee OR. Leaf-specific pathogenesis-related 10 homolog, PgPR-10.3, shows in silico binding affinity with several biologically important molecules. J Ginseng Res. 2015;39(4):406-13doi: 10.1016/j.jgr.2015.06.002.
Han, J. H., Lee, J. H., & Lee, O. R. (2015). Leaf-specific pathogenesis-related 10 homolog, PgPR-10.3, shows in silico binding affinity with several biologically important molecules. Journal of ginseng research, 39(4), 406-13. https://doi.org/10.1016/j.jgr.2015.06.002
Han, Jin Haeng, et al. "Leaf-specific pathogenesis-related 10 homolog, PgPR-10.3, shows in silico binding affinity with several biologically important molecules." Journal of ginseng research vol. 39,4 (2015): 406-13. doi: https://doi.org/10.1016/j.jgr.2015.06.002
Han JH, Lee JH, Lee OR. Leaf-specific pathogenesis-related 10 homolog, PgPR-10.3, shows in silico binding affinity with several biologically important molecules. J Ginseng Res. 2015 Oct;39(4):406-13. doi: 10.1016/j.jgr.2015.06.002. Epub 2015 Jun 19. PMID: 26869835; PMCID: PMC4593852.
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J Ginseng Res. 2015 Oct;39(4):406-13. doi: 10.1016/j.jgr.2015.06.002. Epub 2015 Jun 19.

Leaf-specific pathogenesis-related 10 homolog, PgPR-10.3, shows in silico binding affinity with several biologically important molecules.

Journal of ginseng research

Jin Haeng Han, Jin Hee Lee, Ok Ran Lee

Affiliations

  1. Department of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University, Gwangju, Korea.

PMID: 26869835 PMCID: PMC4593852 DOI: 10.1016/j.jgr.2015.06.002

Abstract

BACKGROUND: Pathogenesis-related 10 (PR-10) proteins are small, cytosolic proteins with a similar three-dimensional structure. Crystal structures for several PR-10 homologs have similar overall folding patterns, with an unusually large internal cavity that is a binding site for biologically important molecules. Although structural information on PR-10 proteins is substantial, understanding of their biological function remains limited. Here, we showed that one of the PgPR-10 homologs, PgPR-10.3, shares binding properties with flavonoids, kinetin, emodin, deoxycholic acid, and ginsenoside Re (1 of the steroid glycosides).

METHODS: Gene expression patterns of PgPR-10.3 were analyzed by quantitative real-time PCR. The three-dimensional structure of PgPR-10 proteins was visualized by homology modeling, and docking to retrieve biologically active molecules was performed using AutoDock4 program.

RESULTS: Transcript levels of PgPR-10.3 expressed in leaves, stems, and roots of 3-wk-old ginseng plantlets were on average 86-fold lower than those of PgPR-10.2. In mature 2-yr-old ginseng plants, the mRNA of PgPR-10.3 is restricted to leaves. Ginsenoside Re production is especially prominent in leaves of Panax ginseng Meyer, and the binding property of PgPR-10.3 with ginsenoside Re suggests that this protein has an important role in the control of secondary metabolism.

CONCLUSION: Although ginseng PR-10.3 gene is expressed in all organs of 3-wk-old plantlets, its expression is restricted to leaves in mature 2-yr-old ginseng plants. The putative binding property of PgPR-10.3 with Re is intriguing. Further verification of binding affinity with other biologically important molecules in the large hydrophobic cavity of PgPR-10.3 may provide an insight into the biological features of PR-10 proteins.

Keywords: Panax ginseng Meyer; abiotic stress; ligand binding; pathogenesis-related-10; salicylic acid

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