Display options
Cite
Sinha P, Pazhamala LT, Singh VK, et al. Identification and Validation of Selected Universal Stress Protein Domain Containing Drought-Responsive Genes in Pigeonpea (Cajanus cajan L.). Front Plant Sci. 2016;6:1065doi: 10.3389/fpls.2015.01065.
Sinha, P., Pazhamala, L. T., Singh, V. K., Saxena, R. K., Krishnamurthy, L., Azam, S., Khan, A. W., & Varshney, R. K. (2015). Identification and Validation of Selected Universal Stress Protein Domain Containing Drought-Responsive Genes in Pigeonpea (Cajanus cajan L.). Frontiers in plant science, 61065. https://doi.org/10.3389/fpls.2015.01065
Sinha, Pallavi, et al. "Identification and Validation of Selected Universal Stress Protein Domain Containing Drought-Responsive Genes in Pigeonpea (Cajanus cajan L.)." Frontiers in plant science vol. 6 (2015): 1065. doi: https://doi.org/10.3389/fpls.2015.01065
Sinha P, Pazhamala LT, Singh VK, Saxena RK, Krishnamurthy L, Azam S, Khan AW, Varshney RK. Identification and Validation of Selected Universal Stress Protein Domain Containing Drought-Responsive Genes in Pigeonpea (Cajanus cajan L.). Front Plant Sci. 2016 Jan 06;6:1065. doi: 10.3389/fpls.2015.01065. eCollection 2015. PMID: 26779199; PMCID: PMC4701917.
Copy Download .nbib Format: NLM
Share it on

Front Plant Sci. 2016 Jan 06;6:1065. doi: 10.3389/fpls.2015.01065. eCollection 2015.

Identification and Validation of Selected Universal Stress Protein Domain Containing Drought-Responsive Genes in Pigeonpea (Cajanus cajan L.).

Frontiers in plant science

Pallavi Sinha, Lekha T Pazhamala, Vikas K Singh, Rachit K Saxena, L Krishnamurthy, Sarwar Azam, Aamir W Khan, Rajeev K Varshney

Affiliations

  1. Center of Excellence in Genomics (CEG), International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) Hyderabad, India.
  2. Center of Excellence in Genomics (CEG), International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)Hyderabad, India; School of Plant Biology and the Institute of Agriculture, The University of Western AustraliaPerth, WA, Australia.

PMID: 26779199 PMCID: PMC4701917 DOI: 10.3389/fpls.2015.01065

Abstract

Pigeonpea is a resilient crop, which is relatively more drought tolerant than many other legume crops. To understand the molecular mechanisms of this unique feature of pigeonpea, 51 genes were selected using the Hidden Markov Models (HMM) those codes for proteins having close similarity to universal stress protein domain. Validation of these genes was conducted on three pigeonpea genotypes (ICPL 151, ICPL 8755, and ICPL 227) having different levels of drought tolerance. Gene expression analysis using qRT-PCR revealed 6, 8, and 18 genes to be ≥2-fold differentially expressed in ICPL 151, ICPL 8755, and ICPL 227, respectively. A total of 10 differentially expressed genes showed ≥2-fold up-regulation in the more drought tolerant genotype, which encoded four different classes of proteins. These include plant U-box protein (four genes), universal stress protein A-like protein (four genes), cation/H(+) antiporter protein (one gene) and an uncharacterized protein (one gene). Genes C.cajan_29830 and C.cajan_33874 belonging to uspA, were found significantly expressed in all the three genotypes with ≥2-fold expression variations. Expression profiling of these two genes on the four other legume crops revealed their specific role in pigeonpea. Therefore, these genes seem to be promising candidates for conferring drought tolerance specifically to pigeonpea.

Keywords: drought responsive genes; expression profiling; in-silico analysis; legumes; pigeonpea

References

  1. Curr Opin Plant Biol. 2009 Apr;12(2):202-10 - PubMed
  2. Plant Cell Rep. 2012 May;31(5):827-38 - PubMed
  3. AoB Plants. 2013;5:plt007 - PubMed
  4. Bioinform Biol Insights. 2011 Feb 07;5:41-58 - PubMed
  5. Plant Physiol. 2006 Dec;142(4):1664-82 - PubMed
  6. Annu Rev Plant Biol. 2014;65:715-41 - PubMed
  7. PLoS One. 2015 Apr 07;10(4):e0122847 - PubMed
  8. Gene. 2007 Feb 15;388(1-2):1-13 - PubMed
  9. Nucleic Acids Res. 2010 Jan;38(Database issue):D211-22 - PubMed
  10. Funct Integr Genomics. 2011 Dec;11(4):651-7 - PubMed
  11. Plant Biotechnol J. 2011 Apr;9(3):315-27 - PubMed
  12. Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):4109-14 - PubMed
  13. J Bacteriol. 2005 Sep;187(18):6265-72 - PubMed
  14. PLoS One. 2013 Oct 22;8(10):e78555 - PubMed
  15. Biochem Biophys Res Commun. 2010 May 28;396(2):283-8 - PubMed
  16. Plant Mol Biol. 2002 Mar-Apr;48(5-6):551-73 - PubMed
  17. Trends Biochem Sci. 2010 Jun;35(6):352-60 - PubMed
  18. Int J Mol Sci. 2013 Mar 28;14(4):7155-79 - PubMed
  19. Bioinformatics. 2005 Aug 15;21(16):3448-9 - PubMed
  20. PLoS Pathog. 2009 May;5(5):e1000460 - PubMed
  21. Plant Physiol. 2009 Jan;149(1):88-95 - PubMed
  22. Annu Rev Plant Biol. 2006;57:781-803 - PubMed
  23. J Biol Chem. 2004 Jul 2;279(27):28539-52 - PubMed
  24. Trends Plant Sci. 2005 Feb;10(2):88-94 - PubMed
  25. Plant Mol Biol. 2009 Jan;69(1-2):133-53 - PubMed
  26. Nat Biotechnol. 2011 Nov 06;30(1):83-9 - PubMed
  27. Plant Cell. 2008 Jul;20(7):1899-914 - PubMed
  28. Mol Genet Genomics. 2009 Jun;281(6):591-605 - PubMed
  29. Biotechniques. 2008 Apr;44(5):619-26 - PubMed
  30. C R Biol. 2014 Feb;337(2):86-94 - PubMed
  31. Theor Appl Genet. 2012 Aug;125(4):625-45 - PubMed
  32. Plant Cell Environ. 2010 Aug 1;33(8):1324-38 - PubMed
  33. Mol Plant. 2012 Sep;5(5):1020-8 - PubMed
  34. Theor Appl Genet. 2014 Feb;127(2):445-62 - PubMed
  35. Plant Physiol. 2006 Apr;140(4):1279-96 - PubMed
  36. Ann Bot. 2010 Jun;105(7):1119-28 - PubMed
  37. Mol Breed. 2012 Aug;30(2):757-772 - PubMed
  38. BMC Plant Biol. 2011 Sep 17;11:127 - PubMed
  39. Plant Cell Environ. 2002 Feb;25(2):141-151 - PubMed
  40. Plant Physiol. 2004 Sep;136(1):2532-47 - PubMed
  41. Mol Genet Genomics. 2010 Mar;283(3):273-87 - PubMed
  42. Biochem Biophys Res Commun. 2003 Mar 21;302(4):635-45 - PubMed

Publication Types