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Martano M, Restucci B, Ceccarelli DM, et al. Immunohistochemical expression of vascular endothelial growth factor in canine oral squamous cell carcinomas. Oncol Lett. 2015;11(1):399-404doi: 10.3892/ol.2015.3847.
Martano, M., Restucci, B., Ceccarelli, D. M., Lo Muzio, L., & Maiolino, P. (2016). Immunohistochemical expression of vascular endothelial growth factor in canine oral squamous cell carcinomas. Oncology letters, 11(1), 399-404. https://doi.org/10.3892/ol.2015.3847
Martano, Manuela, et al. "Immunohistochemical expression of vascular endothelial growth factor in canine oral squamous cell carcinomas." Oncology letters vol. 11,1 (2016): 399-404. doi: https://doi.org/10.3892/ol.2015.3847
Martano M, Restucci B, Ceccarelli DM, Lo Muzio L, Maiolino P. Immunohistochemical expression of vascular endothelial growth factor in canine oral squamous cell carcinomas. Oncol Lett. 2016 Jan;11(1):399-404. doi: 10.3892/ol.2015.3847. Epub 2015 Oct 30. PMID: 26870224; PMCID: PMC4727185.
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Oncol Lett. 2016 Jan;11(1):399-404. doi: 10.3892/ol.2015.3847. Epub 2015 Oct 30.

Immunohistochemical expression of vascular endothelial growth factor in canine oral squamous cell carcinomas.

Oncology letters

Manuela Martano, Brunella Restucci, Dora Maria Ceccarelli, Lorenzo Lo Muzio, Paola Maiolino

Affiliations

  1. Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples 80137, Italy.
  2. Department of Clinical and Experimental Medicine, University of Foggia, Foggia 71122, Italy.

PMID: 26870224 PMCID: PMC4727185 DOI: 10.3892/ol.2015.3847

Abstract

Angiogenesis is crucial for the growth and metastasis of malignant tumours, and various proangiogenic factors promote this process. One of these factors is vascular endothelial growth factor (VEGF), which appears to play a key role in tumour angiogenesis. The aim of the present study was to assess whether VEGF expression is associated with angiogenesis, disease progression and neoplastic proliferation in canine oral squamous cell carcinoma (OSCC) tissue. VEGF immunoreactivity was quantified by immunohistochemistry in 30 specimens, including normal oral mucosa and OSCC tissues graded as well, moderately or poorly differentiated. VEGF expression was correlated with tumour cell proliferation, as assessed using the proliferating cell nuclear antigen (PCNA) marker and microvessel density (data already published). The present results revealed that VEGF and PCNA expression increased significantly between normal oral tissue and neoplastic tissue, and between well and moderately/poorly differentiated tumours. In addition, VEGF expression was strongly correlated with PCNA expression and microvessel density. It was concluded that VEGF may promote angiogenesis through a paracrine pathway, stimulating endothelial cell proliferation and, similarly, may induce tumour cell proliferation through an autocrine pathway. The present results suggest that the evaluation of VEGF may be a useful additional criterion for estimating malignancy and growth potential in canine OSCCs.

Keywords: dog; immunohistochemistry; oral cancer; oral squamous cell carcinoma; vascular endothelial growth factor

References

  1. Vet Pathol. 2007 Nov;44(6):823-30 - PubMed
  2. Anticancer Res. 2009 Aug;29(8):2919-25 - PubMed
  3. Biochim Biophys Acta. 1990 Jun 1;1032(1):89-118 - PubMed
  4. Oncologist. 2005 Jun-Jul;10(6):382-91 - PubMed
  5. J Small Anim Pract. 2011 Jul;52(7):359-64 - PubMed
  6. J Oral Pathol Med. 2005 Mar;34(3):134-9 - PubMed
  7. J Oral Pathol Med. 2011 Oct;40(9):693-8 - PubMed
  8. J Pathol. 1998 Jan;184(1):53-7 - PubMed
  9. Circ Res. 2004 Jun 11;94(11):1443-50 - PubMed
  10. Oral Maxillofac Surg. 2014 Jun;18(2):187-96 - PubMed
  11. J Clin Oncol. 2005 Feb 10;23(5):1011-27 - PubMed
  12. Mod Pathol. 1997 Nov;10(11):1128-33 - PubMed
  13. Clin Cancer Res. 2000 Jul;6(7):2821-8 - PubMed
  14. Acta Vet Hung. 2014 Sep;62(3):362-71 - PubMed
  15. J Comp Pathol. 2004 Feb-Apr;130(2-3):99-104 - PubMed
  16. Oral Oncol. 2004 Jan;40(1):13-20 - PubMed
  17. Histochem J. 2001 May;33(5):287-94 - PubMed
  18. Endocr Rev. 1997 Feb;18(1):4-25 - PubMed
  19. Lab Invest. 1997 Dec;77(6):659-64 - PubMed
  20. J Biol Chem. 1995 Oct 27;270(43):25915-9 - PubMed
  21. Acta Otorhinolaryngol Ital. 2005 Feb;25(1):13-7 - PubMed
  22. J Clin Pathol. 1998 Oct;51(10):771-5 - PubMed
  23. J Natl Cancer Inst. 1990 Jan 3;82(1):4-6 - PubMed
  24. EXS. 1997;79:209-32 - PubMed
  25. J Comp Pathol. 2003 May;128(4):252-9 - PubMed
  26. Nat Med. 2003 Jun;9(6):669-76 - PubMed
  27. Anticancer Res. 2012 Mar;32(3):747-52 - PubMed
  28. J Dent Educ. 2001 Nov;65(11):1282-90 - PubMed
  29. Rom J Morphol Embryol. 2009;50(4):527-48 - PubMed
  30. Cancer Metastasis Rev. 1995 Dec;14(4):263-77 - PubMed
  31. Oral Oncol. 2006 Apr;42(4):337-42 - PubMed
  32. Res Vet Sci. 2006 Dec;81(3):350-7 - PubMed
  33. J Biol Chem. 1998 Mar 13;273(11):6417-23 - PubMed
  34. Nature. 1993 Apr 29;362(6423):841-4 - PubMed
  35. Eur J Cancer B Oral Oncol. 1996 Jan;32B(1):24-31 - PubMed
  36. Anticancer Res. 2007 Sep-Oct;27(5A):3083-9 - PubMed
  37. Vet Res Commun. 2004 Aug;28 Suppl 1:299-301 - PubMed
  38. J Pathol. 1998 Nov;186(3):313-8 - PubMed
  39. J Oral Pathol Med. 1995 Aug;24(7):313-5 - PubMed
  40. J Pathol. 1996 Jul;179(3):232-7 - PubMed
  41. Cancer Res. 1995 Oct 15;55(20):4575-80 - PubMed
  42. Trends Pharmacol Sci. 2001 Apr;22(4):201-7 - PubMed
  43. J Comp Pathol. 2000 Aug-Oct;123(2-3):141-5 - PubMed
  44. Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):233-5 - PubMed
  45. J Am Vet Med Assoc. 1979 Sep 15;175(6):567-71 - PubMed
  46. FASEB J. 1999 Jan;13(1):9-22 - PubMed
  47. Vet Pathol. 2002 Jul;39(4):488-93 - PubMed
  48. Eur J Histochem. 2006 Apr-Jun;50(2):125-30 - PubMed

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