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Harifi Mood E, Meshkat Z, Izadi N, et al. Prevalence of Quinolone Resistance Genes Among Extended-Spectrum B-Lactamase-Producing Escherichia coli in Mashhad, Iran. Jundishapur J Microbiol. 2015;8(12):e16217doi: 10.5812/jjm.16217.
Harifi Mood, E., Meshkat, Z., Izadi, N., Rezaei, M., Amel Jamehdar, S., & Naderi Nasab, M. (2015). Prevalence of Quinolone Resistance Genes Among Extended-Spectrum B-Lactamase-Producing Escherichia coli in Mashhad, Iran. Jundishapur journal of microbiology, 8(12), e16217. https://doi.org/10.5812/jjm.16217
Harifi Mood, Elnaz, et al. "Prevalence of Quinolone Resistance Genes Among Extended-Spectrum B-Lactamase-Producing Escherichia coli in Mashhad, Iran." Jundishapur journal of microbiology vol. 8,12 (2015): e16217. doi: https://doi.org/10.5812/jjm.16217
Harifi Mood E, Meshkat Z, Izadi N, Rezaei M, Amel Jamehdar S, Naderi Nasab M. Prevalence of Quinolone Resistance Genes Among Extended-Spectrum B-Lactamase-Producing Escherichia coli in Mashhad, Iran. Jundishapur J Microbiol. 2015 Dec 07;8(12):e16217. doi: 10.5812/jjm.16217. eCollection 2015 Dec. PMID: 26870307; PMCID: PMC4746706.
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Jundishapur J Microbiol. 2015 Dec 07;8(12):e16217. doi: 10.5812/jjm.16217. eCollection 2015 Dec.

Prevalence of Quinolone Resistance Genes Among Extended-Spectrum B-Lactamase-Producing Escherichia coli in Mashhad, Iran.

Jundishapur journal of microbiology

Elnaz Harifi Mood, Zahra Meshkat, Nafiseh Izadi, Maryam Rezaei, Saeid Amel Jamehdar, Mahboubeh Naderi Nasab

Affiliations

  1. Student Research Committee (SRC), Mashhad University of Medical Sciences, Mashhad, IR Iran; Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran.
  2. Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran.
  3. Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, IR Iran.

PMID: 26870307 PMCID: PMC4746706 DOI: 10.5812/jjm.16217

Abstract

BACKGROUND: Escherichia coli is an important bacterial species based on incidence and associated infection severity. Some E. coli strains produce extended-spectrum beta lactamase (ESBL) and are called ESBL-producing E. coli. These strains are resistant to most classes of cephalosporin and a number of other classes of antibiotics. Plasmids carrying qnr genes have been found to transmit quinolone resistance.

OBJECTIVES: The aim of this study was to determine the frequency of qnr genes in ESBL-producing and non-ESBL-producing E. coli isolated from outpatient and hospitalized patient clinical specimens from Imam Reza hospital in Mashhad, Iran.

MATERIALS AND METHODS: Two hundred E. coli strains, isolated from different clinical specimens were used. ESBL-producing E. coli were detected by determining susceptibility to ceftazidime, cefotaxime, and cefpodoxime with the phenotypic confirmatory test (PCT). PCR analysis was employed to detect the qnrA, qnrB, qnrS, bla TEM , and bla SHV genes.

RESULTS: Eighty-six (43%) isolates were ciprofloxacin-resistant. The PCT identified 85 (42.5%) of 200 E. coli isolates as ESBL-producing. The bla TEM , bla SHV , qnrA, qnrB, and qnrS gene were found in 65 (76.47%), 23 (27%), 63 (31%), 34 (17%), and 14 (7%) isolates, respectively.

CONCLUSIONS: The high prevalence of quinolone resistance genes, which indicates antibiotic resistance, in the Imam Reza Hospital of Mashhad is a major concern. Hence, the antibiotics prescription policy should be revised, and infection control measures should be improved.

Keywords: Escherichia coli; Extended-Spectrum Beta-Lactamas; Quinolone Resistance

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