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Hebbani Nagarajappa S, Pandit S, Divanji M, et al. Effect of Coleus forskohlii and its major constituents on cytochrome P450 induction. J Tradit Complement Med. 2015;6(1):130-3doi: 10.1016/j.jtcme.2014.11.027.
Hebbani Nagarajappa, S., Pandit, S., Divanji, M., Mariyanna, B., Kumar, P., & Godavarthi, A. (2016). Effect of Coleus forskohlii and its major constituents on cytochrome P450 induction. Journal of traditional and complementary medicine, 6(1), 130-3. https://doi.org/10.1016/j.jtcme.2014.11.027
Hebbani Nagarajappa, Shivaprasad, et al. "Effect of Coleus forskohlii and its major constituents on cytochrome P450 induction." Journal of traditional and complementary medicine vol. 6,1 (2016): 130-3. doi: https://doi.org/10.1016/j.jtcme.2014.11.027
Hebbani Nagarajappa S, Pandit S, Divanji M, Mariyanna B, Kumar P, Godavarthi A. Effect of Coleus forskohlii and its major constituents on cytochrome P450 induction. J Tradit Complement Med. 2015 Jan 29;6(1):130-3. doi: 10.1016/j.jtcme.2014.11.027. eCollection 2016 Jan. PMID: 26870691; PMCID: PMC4737967.
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J Tradit Complement Med. 2015 Jan 29;6(1):130-3. doi: 10.1016/j.jtcme.2014.11.027. eCollection 2016 Jan.

Effect of Coleus forskohlii and its major constituents on cytochrome P450 induction.

Journal of traditional and complementary medicine

Shivaprasad Hebbani Nagarajappa, Subrata Pandit, Manohar Divanji, Bhanumathy Mariyanna, Pavan Kumar, Ashok Godavarthi

Affiliations

  1. Research and Development Centre, Olive Lifesciences Pvt. Ltd., Anthrasanahalli, Tumkur, Karnataka, India.
  2. Radiant Research Services Pvt. Ltd., Srinagar, Bangalore, India.

PMID: 26870691 PMCID: PMC4737967 DOI: 10.1016/j.jtcme.2014.11.027

Abstract

Coleus forskohlii Briq. has been used traditionally for the treatment of several ailments since antiquity in Ayurveda. In the present study, an approach has been made to evaluate the effect of C. forskohlii and its major constituents on cytochrome P450 (CYP3A, CYP2B, and CYP2C) mRNA expression in rat hepatocytes. To gain better understanding of the herb-drug interaction potential of the chemical constituents present in C. forskohlii, the extract was subjected to column chromatography followed by standardization with respect to forskolin, 1-deoxyforskolin, and 1,9-dideoxyforskolin using reversed-phase high-performance liquid chromatography (RP-HPLC). Hepatocytes were treated with extracts, fractions, and phytoconstituents, followed by extraction and purification of total mRNA. Study of mRNA expression was carried out through reverse transcription polymerase chain reaction, followed by agarose gel electrophoresis. Results revealed that the test substances did not show any significant mRNA expression compared to the control against CYP3A, CYP2B, and CYP2C. Positive controls such as dexamethasone and rifampin showed significantly high (p < 0.001) induction potential compared to the control. It can be concluded that C. forskohlii and its major constituents may not be involved in CYP450 induction-based drug interaction.

Keywords: Coleus forskohlii; Cytochrome P450; Drug interaction; Standardization; mRNA expression

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