Display options
Cite
Kwon HW, Shin JH, Cho HJ, et al. Total saponin from Korean Red Ginseng inhibits binding of adhesive proteins to glycoprotein IIb/IIIa via phosphorylation of VASP (Ser(157)) and dephosphorylation of PI3K and Akt. J Ginseng Res. 2015;40(1):76-85doi: 10.1016/j.jgr.2015.05.004.
Kwon, H. W., Shin, J. H., Cho, H. J., Rhee, M. H., & Park, H. J. (2016). Total saponin from Korean Red Ginseng inhibits binding of adhesive proteins to glycoprotein IIb/IIIa via phosphorylation of VASP (Ser(157)) and dephosphorylation of PI3K and Akt. Journal of ginseng research, 40(1), 76-85. https://doi.org/10.1016/j.jgr.2015.05.004
Kwon, Hyuk-Woo, et al. "Total saponin from Korean Red Ginseng inhibits binding of adhesive proteins to glycoprotein IIb/IIIa via phosphorylation of VASP (Ser(157)) and dephosphorylation of PI3K and Akt." Journal of ginseng research vol. 40,1 (2016): 76-85. doi: https://doi.org/10.1016/j.jgr.2015.05.004
Kwon HW, Shin JH, Cho HJ, Rhee MH, Park HJ. Total saponin from Korean Red Ginseng inhibits binding of adhesive proteins to glycoprotein IIb/IIIa via phosphorylation of VASP (Ser(157)) and dephosphorylation of PI3K and Akt. J Ginseng Res. 2016 Jan;40(1):76-85. doi: 10.1016/j.jgr.2015.05.004. Epub 2015 May 21. PMID: 26843825; PMCID: PMC4703804.
Copy Download .nbib Format: NLM
Share it on

J Ginseng Res. 2016 Jan;40(1):76-85. doi: 10.1016/j.jgr.2015.05.004. Epub 2015 May 21.

Total saponin from Korean Red Ginseng inhibits binding of adhesive proteins to glycoprotein IIb/IIIa via phosphorylation of VASP (Ser(157)) and dephosphorylation of PI3K and Akt.

Journal of ginseng research

Hyuk-Woo Kwon, Jung-Hae Shin, Hyun-Jeong Cho, Man Hee Rhee, Hwa-Jin Park

Affiliations

  1. Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje University, Gyungnam, Republic of Korea.
  2. Department of Biomedical Laboratory Science, College of Medical Science, Konyang University, Daejeon, Republic of Korea.
  3. Laboratory of Veterinary Physiology and Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.

PMID: 26843825 PMCID: PMC4703804 DOI: 10.1016/j.jgr.2015.05.004

Abstract

BACKGROUND: Binding of adhesive proteins (i.e., fibrinogen, fibronectin, vitronectin) to platelet integrin glycoprotein IIb/IIIa (αIIb/β3) by various agonists (thrombin, collagen, adenosine diphosphate) involve in strength of thrombus. This study was carried out to evaluate the antiplatelet effect of total saponin from Korean Red Ginseng (KRG-TS) by investigating whether KRG-TS inhibits thrombin-induced binding of fibrinogen and fibronectin to αIIb/β3.

METHODS: We investigated the effect of KRG-TS on phosphorylation of vasodilator-stimulated phosphoprotein (VASP) and dephosphorylation of phosphatidylinositol 3-kinase (PI3K) and Akt, affecting binding of fibrinogen and fibronectin to αIIb/β3, and clot retraction.

RESULTS: KRG-TS had an antiplatelet effect by inhibiting the binding of fibrinogen and fibronectin to αIIb/β3 via phosphorylation of VASP (Ser(157)), and dephosphorylation of PI3K and Akt on thrombin-induced platelet aggregation. Moreover, A-kinase inhibitor Rp-8-Br-cyclic adenosine monophosphates (cAMPs) reduced KRG-TS-increased VASP (Ser(157)) phosphorylation, and increased KRG-TS-inhibited fibrinogen-, and fibronectin-binding to αIIb/β3. These findings indicate that KRG-TS interferes with the binding of fibrinogen and fibronectin to αIIb/β3 via cAMP-dependent phosphorylation of VASP (Ser(157)). In addition, KRG-TS decreased the rate of clot retraction, reflecting inhibition of αIIb/β3 activation. In this study, we clarified ginsenoside Ro (G-Ro) in KRG-TS inhibited thrombin-induced platelet aggregation via both inhibition of [Ca(2+)]i mobilization and increase of cAMP production.

CONCLUSION: These results strongly indicate that KRG-TS is a beneficial herbal substance inhibiting fibrinogen-, and fibronectin-binding to αIIb/β3, and clot retraction, and may prevent platelet αIIb/β3-mediated thrombotic disease. In addition, we demonstrate that G-Ro is a novel compound with antiplatelet characteristics of KRG-TS.

Keywords: PI3K/Akt; VASP (Ser157); adhesive protein; clot retraction; total saponin from Korean Red Ginseng

References

  1. J Cell Biol. 1999 Mar 22;144(6):1245-58 - PubMed
  2. Science. 1999 Jun 18;284(5422):1994-8 - PubMed
  3. Nature. 1999 Oct 21;401(6755):808-11 - PubMed
  4. J Am Coll Cardiol. 2000 Apr;35(5):1103-15 - PubMed
  5. Am J Med. 2000 Aug 15;109(3):224-37 - PubMed
  6. Biochem Pharmacol. 2000 Oct 15;60(8):1069-74 - PubMed
  7. Thromb Haemost. 2001 Jul;86(1):246-58 - PubMed
  8. Crit Care Med. 2002 May;30(5 Suppl):S332-40 - PubMed
  9. Catheter Cardiovasc Interv. 2003 Jul;59(3):295-302 - PubMed
  10. Platelets. 2003 Sep;14(6):381-90 - PubMed
  11. Blood. 2004 Sep 15;104(6):1703-10 - PubMed
  12. Blood. 2004 Sep 15;104(6):1606-15 - PubMed
  13. Circ Res. 2004 Dec 10;95(12):1225-33 - PubMed
  14. J Thromb Haemost. 2005 Aug;3(8):1569-76 - PubMed
  15. J Thromb Haemost. 2005 Aug;3(8):1577-89 - PubMed
  16. Basic Clin Pharmacol Toxicol. 2007 Mar;100(3):170-5 - PubMed
  17. Circ Res. 2007 Jun 22;100(12):1673-85 - PubMed
  18. Phytother Res. 2008 Jun;22(6):778-83 - PubMed
  19. Biochem J. 1991 Jan 1;273(Pt 1):115-20 - PubMed
  20. Acta Pharmacol Sin. 2008 Sep;29(9):1109-18 - PubMed
  21. J Pharm Pharmacol. 2008 Nov;60(11):1531-6 - PubMed
  22. Cardiovasc Res. 2009 May 1;82(2):261-71 - PubMed
  23. Arch Pharm Res. 2009 Jun;32(6):813-22 - PubMed
  24. Thromb Haemost. 2009 Aug;102(2):327-35 - PubMed
  25. J Mol Cell Cardiol. 2010 Sep;49(3):532-42 - PubMed
  26. Physiol Rev. 1990 Oct;70(4):1177-209 - PubMed
  27. Br J Pharmacol. 2012 Sep;167(1):109-27 - PubMed
  28. J Atheroscler Thromb. 2012;19(4):337-48 - PubMed
  29. J Ginseng Res. 2011 Sep;35(3):325-30 - PubMed
  30. J Ginseng Res. 2011 Nov;35(4):389-98 - PubMed
  31. J Ginseng Res. 2012 Jan;36(1):40-6 - PubMed
  32. J Ginseng Res. 2013 Apr;37(2):176-86 - PubMed
  33. J Atheroscler Thromb. 2014;21(1):23-37 - PubMed
  34. Arterioscler Thromb Vasc Biol. 2015 Jan;35(1):24-9 - PubMed
  35. J Ginseng Res. 2014 Jul;38(3):161-6 - PubMed
  36. J Ginseng Res. 2014 Jul;38(3):208-14 - PubMed
  37. J Ginseng Res. 2015 Jan;39(1):61-8 - PubMed
  38. J Ginseng Res. 2015 Oct;39(4):354-64 - PubMed
  39. J Ginseng Res. 2015 Oct;39(4):365-70 - PubMed
  40. Biochim Biophys Acta. 1989 Mar 24;990(3):315-20 - PubMed
  41. Semin Hematol. 1986 Jan;23(1):8-26 - PubMed
  42. Hum Pathol. 1987 Mar;18(3):240-7 - PubMed
  43. Chem Pharm Bull (Tokyo). 1986 Mar;34(3):1153-7 - PubMed
  44. Chem Pharm Bull (Tokyo). 1986 May;34(5):2100-4 - PubMed
  45. J Biol Chem. 1985 Mar 25;260(6):3440-50 - PubMed
  46. J Biol Chem. 1981 Sep 25;256(18):9477-82 - PubMed
  47. Prog Cardiovasc Dis. 1984 Mar-Apr;26(5):355-72 - PubMed
  48. J Cell Biol. 1982 Feb;92(2):584-8 - PubMed
  49. Blood. 1981 Apr;57(4):781-7 - PubMed
  50. Eur J Biochem. 1994 Oct 1;225(1):21-7 - PubMed
  51. J Biol Chem. 1996 Mar 15;271(11):6265-72 - PubMed
  52. J Biol Chem. 1998 Aug 7;273(32):20029-35 - PubMed

Publication Types