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Beaulieu L, Thibodeau J, Desbiens M, et al. Evidence of Antibacterial Activities in Peptide Fractions Originating from Snow Crab (Chionoecetes opilio) By-Products. Probiotics Antimicrob Proteins. 2010;2(3):197-209doi: 10.1007/s12602-010-9043-6.
Beaulieu, L., Thibodeau, J., Desbiens, M., Saint-Louis, R., Zatylny-Gaudin, C., & Thibault, S. (2010). Evidence of Antibacterial Activities in Peptide Fractions Originating from Snow Crab (Chionoecetes opilio) By-Products. Probiotics and antimicrobial proteins, 2(3), 197-209. https://doi.org/10.1007/s12602-010-9043-6
Beaulieu, Lucie, et al. "Evidence of Antibacterial Activities in Peptide Fractions Originating from Snow Crab (Chionoecetes opilio) By-Products." Probiotics and antimicrobial proteins vol. 2,3 (2010): 197-209. doi: https://doi.org/10.1007/s12602-010-9043-6
Beaulieu L, Thibodeau J, Desbiens M, Saint-Louis R, Zatylny-Gaudin C, Thibault S. Evidence of Antibacterial Activities in Peptide Fractions Originating from Snow Crab (Chionoecetes opilio) By-Products. Probiotics Antimicrob Proteins. 2010 Oct;2(3):197-209. doi: 10.1007/s12602-010-9043-6. PMID: 26781242.
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Probiotics Antimicrob Proteins. 2010 Oct;2(3):197-209. doi: 10.1007/s12602-010-9043-6.

Evidence of Antibacterial Activities in Peptide Fractions Originating from Snow Crab (Chionoecetes opilio) By-Products.

Probiotics and antimicrobial proteins

Lucie Beaulieu, Jacinthe Thibodeau, Michel Desbiens, Richard Saint-Louis, Céline Zatylny-Gaudin, Sharon Thibault

Affiliations

  1. Université du Québec à Rimouski (UQAR), 300 allée des Ursulines, Rimouski, QC, G5L 3A1, Canada. [email protected].
  2. Institute of Nutraceuticals and Functional Food (INAF), Université Laval, Québec, QC, G1V 0A6, Canada. [email protected].
  3. Université du Québec à Rimouski (UQAR), 300 allée des Ursulines, Rimouski, QC, G5L 3A1, Canada.
  4. Aquatic Products Technology Centre (CTPA, MAPAQ), 96, montée de Sandy Beach, office 1.07, Gaspé, QC, G4X 2V6, Canada.
  5. Institut des sciences de la mer (ISMER, UQAR), 310 allée des Ursulines, Rimouski, QC, G5L 3A1, Canada.
  6. Université de Caen, Esplanade de la Paix, 14032, Caen cedex, France.

PMID: 26781242 DOI: 10.1007/s12602-010-9043-6

Abstract

Antibacterial peptide fractions generated via proteolytic processing of snow crab by-products exhibited activity against Gram-negative and Gram-positive bacteria. Among the bacterial strains tested, peptide fractions demonstrated inhibitory activity against the Gram-negative bacteria such as Aeromonas caviae, Aeromonas hydrophila, Campylobacter jejuni, Listonella anguillarum, Morganella morganii, Shewanella putrefasciens, Vibrio parahaemolyticus and Vibrio vulnificus and against a few Gram-positive bacteria such as Listeria monocytogenes, Staphylococcus epidermidis and Streptococcus agalactiae. The principal bioactive peptide fraction was comprised mainly of proteins and minerals (74.3 and 15.5%, respectively). Lipids were not detected. The amino acid content revealed that arginine (4.6%), glutamic acid (5.3%) and tyrosine (4.8%) residues were represented in the highest composition in the antibacterial peptide fraction. The optimal inhibitory activity was observed at alkaline pH. The V. vulnificus strain, most sensitive to the peptide fraction, was used to develop purification methods. The most promising chromatography resins selected for purification, in order to isolate peptides of interest and to carry out their detailed biochemical characterization, were the SP-Sepharose™ Fast Flow cation exchanger and the Phenyl Sepharose™ High Performance hydrophobic interaction media. The partially purified antibacterial peptide fraction was analyzed for minimum inhibitory concentration (MIC) determination, and the value obtained was 25 μg ml(-1). Following mass spectrometry analysis, the active peptide fraction seems to be a complex of molecules comprised of several amino acids and other organic compounds. In addition, copper was the main metal found in the active peptide fraction. Results indicate the production of antibacterial molecules from crustacean by-products that support further applications for high-value bioproducts in several areas such as food and health.

Keywords: Antibacterial activities; Peptide fractions; Snow crab by-products

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