Display options
Cite
Baranov MV, Ter Beest M, van den Bogaart G. Reaching for far-flung antigen: How solid-core podosomes of dendritic cells transform into protrusive structures. Commun Integr Biol. 2014;7(5)doi: 10.4161/cib.29084.
Baranov, M. V., Ter Beest, M., & van den Bogaart, G. (2014). Reaching for far-flung antigen: How solid-core podosomes of dendritic cells transform into protrusive structures. Communicative & integrative biology, 7(5), . https://doi.org/10.4161/cib.29084
Baranov, Maksim V, et al. "Reaching for far-flung antigen: How solid-core podosomes of dendritic cells transform into protrusive structures." Communicative & integrative biology vol. 7,5 (2014). doi: https://doi.org/10.4161/cib.29084
Baranov MV, Ter Beest M, van den Bogaart G. Reaching for far-flung antigen: How solid-core podosomes of dendritic cells transform into protrusive structures. Commun Integr Biol. 2014 Nov 11;7(5). doi: 10.4161/cib.29084. eCollection 2014 Oct. PMID: 26843902; PMCID: PMC4594491.
Copy Download .nbib Format: NLM
Share it on

Commun Integr Biol. 2014 Nov 11;7(5). doi: 10.4161/cib.29084. eCollection 2014 Oct.

Reaching for far-flung antigen: How solid-core podosomes of dendritic cells transform into protrusive structures.

Communicative & integrative biology

Maksim V Baranov, Martin Ter Beest, Geert van den Bogaart

Affiliations

  1. Department of Tumor Immunology; Radboud University Medical Center ; Radboud Institute for Molecular Life Sciences ; Nijmegen, The Netherlands.

PMID: 26843902 PMCID: PMC4594491 DOI: 10.4161/cib.29084

Abstract

We recently identified a novel role for podosomes in antigen sampling. Podosomes are dynamic cellular structures that consist of point-like concentrations of actin surrounded by integrins and adaptor proteins such as vinculin and talin. Podosomes establish cellular contact with the extracellular matrix (ECM) and facilitate cell migration via ECM degradation. In our recent paper, we studied podosomes of human dendritic cells (DCs), major antigen presenting cells (APC) that take-up, process, and present foreign antigen to naive T-cells. We employed gelatin-impregnated porous polycarbonate filters to demonstrate that the mechanosensitive podosomes of DCs selectively localize to regions of low-physical resistance such as the filter pores. After degradation of the gelatin, podosomes increasingly protrude into the lumen of these pores. These protrusive podosome-derived structures contain several endocytic and early endosomal markers such as clathrin, Rab5, and VAMP3, and, surprisingly, also contain C-type lectins, a type of pathogen recognition receptors (PRRs). Finally, we performed functional uptake experiments to demonstrate that these PRRs facilitate uptake of antigen from the opposite side of the filter. Our data provide mechanistic insight in how dendritic cells sample for antigen across epithelial barriers for instance from the lumen of the lung and gut.

Keywords: Actin; Antigen sampling; C-type lectins; Dendritic cell; Invadopodia; Leukocyte extravasation; Membrane trafficking; Pattern recognition receptors; Podosome; Receptor-mediated endocytosis

References

  1. Immunity. 2013 Mar 21;38(3):581-95 - PubMed
  2. Annu Rev Cell Dev Biol. 2011;27:185-211 - PubMed
  3. J Cell Sci. 2010 May 1;123(Pt 9):1427-37 - PubMed
  4. J Cell Biol. 2000 May 1;149(3):697-706 - PubMed
  5. Nat Immunol. 2001 Apr;2(4):361-7 - PubMed
  6. J Cell Sci. 2009 Nov 1;122(Pt 21):3873-82 - PubMed
  7. Blood. 2001 Aug 15;98(4):1142-9 - PubMed
  8. Trends Cell Biol. 2007 Mar;17(3):107-17 - PubMed
  9. Mol Biol Cell. 2011 Jan 15;22(2):202-15 - PubMed
  10. Proc Natl Acad Sci U S A. 2010 Dec 7;107(49):21016-21 - PubMed
  11. Gastroenterology. 2012 Mar;142(3):592-601.e3 - PubMed
  12. Blood. 2010 Sep 2;116(9):1559-69 - PubMed
  13. Clin Exp Metastasis. 2009;26(4):289-98 - PubMed
  14. J Cell Biol. 2008 Jun 16;181(6):985-98 - PubMed
  15. Curr Biol. 2008 Sep 9;18(17 ):1288-94 - PubMed
  16. J Exp Med. 2012 Jun 4;209(6):1183-99 - PubMed
  17. Curr Opin Immunol. 2005 Feb;17(1):18-24 - PubMed
  18. Curr Opin Hematol. 2012 May;19(3):212-7 - PubMed
  19. J Cell Sci. 2014 Mar 1;127(Pt 5):1052-64 - PubMed
  20. J Cell Biol. 2013 Jul 22;202(2):191-9 - PubMed
  21. Science. 2005 Jan 14;307(5707):254-8 - PubMed
  22. Annu Rev Pathol. 2011;6:323-44 - PubMed
  23. Nat Commun. 2013;4:1412 - PubMed
  24. Nature. 1993 Jul 22;364(6435):346-9 - PubMed
  25. Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9559-64 - PubMed
  26. Physiol Rev. 2002 Jan;82(1):97-130 - PubMed
  27. Nat Methods. 2008 Jul;5(7):605-7 - PubMed
  28. J Crohns Colitis. 2013 Aug;7(7):534-41 - PubMed
  29. Physiol Rev. 2011 Jan;91(1):119-49 - PubMed
  30. Science. 2013 Oct 25;342(6157):447-53 - PubMed
  31. Cytoskeleton (Hoboken). 2013 Oct;70(10):572-89 - PubMed
  32. J Exp Med. 2006 Dec 25;203(13):2841-52 - PubMed
  33. Cell Mol Life Sci. 2012 Jun;69(11):1889-901 - PubMed
  34. Crit Rev Immunol. 2000;20(5):393-405 - PubMed
  35. Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9648-53 - PubMed
  36. Curr Opin Cell Biol. 2008 Apr;20(2):235-41 - PubMed
  37. J Immunol. 2006 Aug 1;177(3):1567-74 - PubMed
  38. J Cell Sci. 2000 Jan;113 Pt 2:183-92 - PubMed
  39. Nat Rev Mol Cell Biol. 2004 Aug;5(8):647-57 - PubMed
  40. Nature. 1998 Mar 19;392(6673):245-52 - PubMed
  41. J Immunol. 2006 Feb 15;176(4):2465-9 - PubMed
  42. Blood. 2005 May 15;105(10):3956-64 - PubMed
  43. J Cell Sci. 2006 Jun 1;119(Pt 11):2375-85 - PubMed
  44. Immunity. 2007 Jun;26(6):784-97 - PubMed

Publication Types