F1000Res. 2015 Oct 28;4:1160. doi: 10.12688/f1000research.7265.2. eCollection 2015.
F1000Research
Goran Kungulovski, Albert Jeltsch
PMID: 26834995 PMCID: PMC4706057 DOI: 10.12688/f1000research.7265.2
Histone post-translational modification (PTM) antibodies are essential research reagents in chromatin biology. However, they suffer from variable properties and insufficient documentation of quality. Antibody manufacturers and vendors should provide detailed lot-specific documentation of quality, rendering further quality checks by end-customers unnecessary. A shift from polyclonal antibodies towards sustainable reagents like monoclonal or recombinant antibodies or histone binding domains would help to improve the reproducibility of experimental work in this field.
Keywords: antibodies; histone modifications; quality control; recombinant proteins