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Kungulovski G, Jeltsch A. Quality of histone modification antibodies undermines chromatin biology research. F1000Res. 2015;4:1160doi: 10.12688/f1000research.7265.2.
Kungulovski, G., & Jeltsch, A. (2015). Quality of histone modification antibodies undermines chromatin biology research. F1000Research, 41160. https://doi.org/10.12688/f1000research.7265.2
Kungulovski, Goran, and Jeltsch, Albert. "Quality of histone modification antibodies undermines chromatin biology research." F1000Research vol. 4 (2015): 1160. doi: https://doi.org/10.12688/f1000research.7265.2
Kungulovski G, Jeltsch A. Quality of histone modification antibodies undermines chromatin biology research. F1000Res. 2015 Oct 28;4:1160. doi: 10.12688/f1000research.7265.2. eCollection 2015. PMID: 26834995; PMCID: PMC4706057.
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F1000Res. 2015 Oct 28;4:1160. doi: 10.12688/f1000research.7265.2. eCollection 2015.

Quality of histone modification antibodies undermines chromatin biology research.

F1000Research

Goran Kungulovski, Albert Jeltsch

Affiliations

  1. Institute of Biochemistry, Stuttgart University, Stuttgart, 70569, Germany.

PMID: 26834995 PMCID: PMC4706057 DOI: 10.12688/f1000research.7265.2

Abstract

Histone post-translational modification (PTM) antibodies are essential research reagents in chromatin biology. However, they suffer from variable properties and insufficient documentation of quality. Antibody manufacturers and vendors should provide detailed lot-specific documentation of quality, rendering further quality checks by end-customers unnecessary. A shift from polyclonal antibodies towards sustainable reagents like monoclonal or recombinant antibodies or histone binding domains would help to improve the reproducibility of experimental work in this field.

Keywords: antibodies; histone modifications; quality control; recombinant proteins

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