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Mishra SK, Malik RK, Manju G, et al. Characterization of a Reuterin-Producing Lactobacillus reuteri BPL-36 Strain Isolated from Human Infant Fecal Sample. Probiotics Antimicrob Proteins. 2012;4(3):154-61doi: 10.1007/s12602-012-9103-1.
Mishra, S. K., Malik, R. K., Manju, G., Pandey, N., Singroha, G., Behare, P., & Kaushik, J. K. (2012). Characterization of a Reuterin-Producing Lactobacillus reuteri BPL-36 Strain Isolated from Human Infant Fecal Sample. Probiotics and antimicrobial proteins, 4(3), 154-61. https://doi.org/10.1007/s12602-012-9103-1
Mishra, Santosh Kumar, et al. "Characterization of a Reuterin-Producing Lactobacillus reuteri BPL-36 Strain Isolated from Human Infant Fecal Sample." Probiotics and antimicrobial proteins vol. 4,3 (2012): 154-61. doi: https://doi.org/10.1007/s12602-012-9103-1
Mishra SK, Malik RK, Manju G, Pandey N, Singroha G, Behare P, Kaushik JK. Characterization of a Reuterin-Producing Lactobacillus reuteri BPL-36 Strain Isolated from Human Infant Fecal Sample. Probiotics Antimicrob Proteins. 2012 Sep;4(3):154-61. doi: 10.1007/s12602-012-9103-1. PMID: 26782041.
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Probiotics Antimicrob Proteins. 2012 Sep;4(3):154-61. doi: 10.1007/s12602-012-9103-1.

Characterization of a Reuterin-Producing Lactobacillus reuteri BPL-36 Strain Isolated from Human Infant Fecal Sample.

Probiotics and antimicrobial proteins

Santosh Kumar Mishra, R K Malik, G Manju, Neha Pandey, Garima Singroha, Pradip Behare, J K Kaushik

Affiliations

  1. Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, India.
  2. Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, India. [email protected].
  3. Animal Biotechnology Centre, National Dairy Research Institute, Karnal, 132001, India.

PMID: 26782041 DOI: 10.1007/s12602-012-9103-1

Abstract

A reuterin (3-hydroxypropinaldehyde, 3-HPA)-producing isolate from a human infant fecal sample was identified as Lactobacillus reuteri BPL-36 strain. The organism displayed a broad-spectrum antimicrobial activity. The gene (gdh) encoding a glycerol dehydratase subunit was detected by PCR, thus confirming its reuterin-producing ability. Reuterin concentration of 89.63 mM/mL was obtained in the MRS-glycerol medium after 16 h of incubation at 37 °C. The reuterin concentration required to inhibit the growth of Pseudomonas aeruginosa, Escherichia coli O157: H7, Salmonella typhi, Staphylococcus aureus, and Listeria monocytogenes was found to be 1.0, 2.0, 2.0, 4.0, and 10.0 AU/mL, respectively. Antimicrobial efficiency test using BPL-36 cell-free supernatant co-incubated along with different test pathogens was done. Viability of all the tested pathogens decreased with increasing contact time with the cell-free supernatant. S. typhi was observed to be the most susceptible among the tested organisms, and the number of viable cells hugely declined as the contact with cell-free supernatant continued, resulting in a reduction of 6 log cycles (100 % inhibition) of the cells after 4 h of treatment. Production of biogenic amines and degradation of mucin by the reuterin-producing BPL-36 strain were not detected.

Keywords: Antimicrobials; Lactobacillus reuteri; Pathogens; Reuterin; Safety

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