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Polina NF, Shkarupeta MM, Popenko AS, et al. Cyto-Insectotoxin 1a from Lachesana tarabaevi Spider Venom Inhibits Chlamydia trachomatis Infection. Probiotics Antimicrob Proteins. 2012;4(3):208-16doi: 10.1007/s12602-012-9108-9.
Polina, N. F., Shkarupeta, M. M., Popenko, A. S., Vassilevski, A. A., Kozlov, S. A., Grishin, E. V., Lazarev, V. N., & Govorun, V. M. (2012). Cyto-Insectotoxin 1a from Lachesana tarabaevi Spider Venom Inhibits Chlamydia trachomatis Infection. Probiotics and antimicrobial proteins, 4(3), 208-16. https://doi.org/10.1007/s12602-012-9108-9
Polina, Nadezhda F, et al. "Cyto-Insectotoxin 1a from Lachesana tarabaevi Spider Venom Inhibits Chlamydia trachomatis Infection." Probiotics and antimicrobial proteins vol. 4,3 (2012): 208-16. doi: https://doi.org/10.1007/s12602-012-9108-9
Polina NF, Shkarupeta MM, Popenko AS, Vassilevski AA, Kozlov SA, Grishin EV, Lazarev VN, Govorun VM. Cyto-Insectotoxin 1a from Lachesana tarabaevi Spider Venom Inhibits Chlamydia trachomatis Infection. Probiotics Antimicrob Proteins. 2012 Sep;4(3):208-16. doi: 10.1007/s12602-012-9108-9. PMID: 26782047.
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Probiotics Antimicrob Proteins. 2012 Sep;4(3):208-16. doi: 10.1007/s12602-012-9108-9.

Cyto-Insectotoxin 1a from Lachesana tarabaevi Spider Venom Inhibits Chlamydia trachomatis Infection.

Probiotics and antimicrobial proteins

Nadezhda F Polina, Marina M Shkarupeta, Anna S Popenko, Alexander A Vassilevski, Sergey A Kozlov, Eugene V Grishin, Vassili N Lazarev, Vadim M Govorun

Affiliations

  1. Research Institute for Physico-Chemical Medicine of the Federal Medical-Biological Agency of Russian Federation, 1a Malaya Pirogovskaya st., 119435, Moscow, Russia.
  2. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya st., 117997, Moscow, Russia.
  3. Research Institute for Physico-Chemical Medicine of the Federal Medical-Biological Agency of Russian Federation, 1a Malaya Pirogovskaya st., 119435, Moscow, Russia. [email protected].
  4. National Research Centre "Kurchatov Institute", 1 Akademika Kurchatova sq., 123182, Moscow, Russia. [email protected].
  5. National Research Centre "Kurchatov Institute", 1 Akademika Kurchatova sq., 123182, Moscow, Russia.

PMID: 26782047 DOI: 10.1007/s12602-012-9108-9

Abstract

Venom of the ant spider Lachesana tarabaevi contains a wide variety of antimicrobial peptides. Among them, a special place belongs to cyto-insectotoxins, a class of cytolytic molecules showing equally potent antimicrobial and insecticidal effects. We tested one of them, CIT 1a, for ability to suppress Chlamydia trachomatis infection. HEK293 cells were transfected with plasmid vectors harboring the cit 1a gene. Controlled expression of the transgene led to a significant decrease in C. trachomatis viability inside the infected cells. Using proteomic and transcriptomic approaches, we found alterations in protein expression patterns and identified differentially expressed genes in transfected cells.

Keywords: Antimicrobial peptide; Cytolytic peptide; Gene therapy; Infectious disease; Proteomics; Transcriptome

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