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Zhang LQ, Sun SL, Li WY, et al. Decreased expression of tumor suppressive miR-874 and its clinical significance in human osteosarcoma. Genet Mol Res. 2015;14(4):18315-24doi: 10.4238/2015.December.23.19.
Zhang, L. Q., Sun, S. L., Li, W. Y., Feng, Z., Xu, X. Y., Zhuang, Q. S., & Fang, J. (2015). Decreased expression of tumor suppressive miR-874 and its clinical significance in human osteosarcoma. Genetics and molecular research : GMR, 14(4), 18315-24. https://doi.org/10.4238/2015.December.23.19
Zhang, L Q, et al. "Decreased expression of tumor suppressive miR-874 and its clinical significance in human osteosarcoma." Genetics and molecular research : GMR vol. 14,4 (2015): 18315-24. doi: https://doi.org/10.4238/2015.December.23.19
Zhang LQ, Sun SL, Li WY, Feng Z, Xu XY, Zhuang QS, Fang J. Decreased expression of tumor suppressive miR-874 and its clinical significance in human osteosarcoma. Genet Mol Res. 2015 Dec 28;14(4):18315-24. doi: 10.4238/2015.December.23.19. PMID: 26782479.
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Genet Mol Res. 2015 Dec 28;14(4):18315-24. doi: 10.4238/2015.December.23.19.

Decreased expression of tumor suppressive miR-874 and its clinical significance in human osteosarcoma.

Genetics and molecular research : GMR

L Q Zhang, S L Sun, W Y Li, Z Feng, X Y Xu, Q S Zhuang, J Fang

Affiliations

  1. Department of Orthopedics, Yidu Central Hospital of Weifang, Weifang, Shandong, China.
  2. Department of Orthopedics, The People's Hospital of Linqu, Weifang, Shandong, China.
  3. Department of Surgery, Health Center in Sitou Town, Weifang, Shandong, China.
  4. Department of Oncology, Qingzhou Chinese Medicine Hospital, Qingzhou, Shandong, China.
  5. Department of Orthopedics, The People's Hospital of Weifang, Shandong, China.

PMID: 26782479 DOI: 10.4238/2015.December.23.19

Abstract

Dysregulation of microRNAs (miRs) is associated with cancer development and progression and aberrant expression of miR-874 have been found in some types of cancer. However, the expression and function of miR-874 in osteosarcoma remain unclear. The aim of this study was to explore the effects of miR-874 in osteosarcoma tumorigenesis and development. The expression level of miR-874 was quantified by real-time reverse transcription-polymerase chain reaction (RT-PCR) in human osteosarcoma cell lines and tissues. Using a miR-874 mimic, cell proliferation and migration assays were performed in an osteosarcoma cell line and tumorigenicity was observed in vivo in order to determine the effects of miR-874 in osteosarcoma cell lines and tissues. MiR-874 was significantly downregulated in osteosarcoma cell lines and clinical specimens. Decreased miR-874 expression was significantly associated with large tumor size, distant metastasis, and advanced clinical stage, and was an independent predictor of poor survival. Overexpression of miR-874 inhibited cell proliferation, invasion and migration in vitro, promoted cell apoptosis in vitro, and suppressed tumorigenicity in vivo. These findings indicate that miR-874 may act as a tumor suppressor in osteosarcoma and could serve as a novel therapeutic agent for miR-based therapy.

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